Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves
It is known that nitrate inhibits ruminal methanogenesis, mainly through competition with hydrogenotrophic methanogens for available hydrogen (H2) and also through toxic effects on the methanogens. However, there is limited knowledge about its effects on the others members of ruminal microbiota and...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2021-12-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654521001657 |
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| author | Abimael Ortiz-Chura José Gere Gisela Marcoppido Gustavo Depetris Silvio Cravero Claudia Faverín Cesar Pinares-Patiño Angel Cataldi María E. Cerón-Cucchi |
| author_facet | Abimael Ortiz-Chura José Gere Gisela Marcoppido Gustavo Depetris Silvio Cravero Claudia Faverín Cesar Pinares-Patiño Angel Cataldi María E. Cerón-Cucchi |
| author_sort | Abimael Ortiz-Chura |
| collection | DOAJ |
| description | It is known that nitrate inhibits ruminal methanogenesis, mainly through competition with hydrogenotrophic methanogens for available hydrogen (H2) and also through toxic effects on the methanogens. However, there is limited knowledge about its effects on the others members of ruminal microbiota and their metabolites. In this study, we investigated the effects of dietary nitrate inclusion on enteric methane (CH4) emission, temporal changes in ruminal microbiota, and fermentation in Holstein calves. Eighteen animals were maintained in individual pens for 45 d. Animals were randomly allocated to either a control (CTR) or nitrate (NIT, containing 15 g of calcium nitrate/kg dry matter) diets. Methane emissions were estimated using the sulfur hexafluoride (SF6) tracer method. Ruminal microbiota changes and ruminal fermentation were evaluated at 0, 4, and 8 h post-feeding. In this study, feed dry matter intake (DMI) did not differ between dietary treatments (P > 0.05). Diets containing NIT reduced CH4 emissions by 27% (g/d) and yield by 21% (g/kg DMI) compared to the CTR (P < 0.05). The pH values and total volatile fatty acids (VFA) concentration did not differ between dietary treatments (P > 0.05) but differed with time, and post-feeding (P < 0.05). Increases in the concentrations of ruminal ammonia nitrogen (NH3–N) and acetate were observed, whereas propionate decreased at 4 h post-feeding with the NIT diet (P < 0.05). Feeding the NIT diet reduced the populations of total bacteria, total methanogens, Ruminococcus albus and Ruminococcus flavefaciens, and the abundance of Succiniclasticum, Coprococcus, Treponema, Shuttlewortia, Succinivibrio, Sharpea, Pseudobutyrivibrio, and Selenomona (P < 0.05); whereas, the population of total fungi, protozoa, Fibrobacter succinogenes, Atopobium and Erysipelotrichaceae L7A_E11 increased (P < 0.05). In conclusion, feeding nitrate reduces enteric CH4 emissions and the methanogens population, whereas it decreases the propionate concentration and the abundance of bacteria involved in the succinate and acrylate pathways. Despite the altered fermentation profile and ruminal microbiota, DMI was not influenced by dietary nitrate. These findings suggest that nitrate has a predominantly direct effect on the reduction of methanogenesis and propionate synthesis. |
| first_indexed | 2024-12-21T00:59:12Z |
| format | Article |
| id | doaj.art-a69ef153b42f4c18843920765ce40751 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-12-21T00:59:12Z |
| publishDate | 2021-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-a69ef153b42f4c18843920765ce407512022-12-21T19:21:15ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452021-12-017412051218Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calvesAbimael Ortiz-Chura0José Gere1Gisela Marcoppido2Gustavo Depetris3Silvio Cravero4Claudia Faverín5Cesar Pinares-Patiño6Angel Cataldi7María E. Cerón-Cucchi8Institute of Pathobiology, CICVyA National Institute of Agricultural Technology, IPVet, UEDD INTA-CONICET, Hurlingham, C1686, ArgentinaEngineering Research and Development Division, National Technological University (UTN), National Scientific and Technical Research Council (CONICET), Buenos Aires, C1179, ArgentinaInstitute of Pathobiology, CICVyA National Institute of Agricultural Technology, IPVet, UEDD INTA-CONICET, Hurlingham, C1686, ArgentinaAgricultural Experimental Station of Balcarce, National Institute of Agricultural Technology (INTA), Balcarce, B7620, ArgentinaInstitute of Agrobiotechnology and Molecular Biology, IABIMO, National Institute of Agricultural Technology (INTA), National Scientific and Technical Research Council (CONICET), Hurlingham, C1686, ArgentinaAgricultural Experimental Station of Balcarce, National Institute of Agricultural Technology (INTA), Balcarce, B7620, ArgentinaThe Agribusiness Group, Lincoln University, PO Box 85016, Lincoln, 7674, New ZealandInstitute of Agrobiotechnology and Molecular Biology, IABIMO, National Institute of Agricultural Technology (INTA), National Scientific and Technical Research Council (CONICET), Hurlingham, C1686, ArgentinaInstitute of Pathobiology, CICVyA National Institute of Agricultural Technology, IPVet, UEDD INTA-CONICET, Hurlingham, C1686, Argentina; Corresponding author.It is known that nitrate inhibits ruminal methanogenesis, mainly through competition with hydrogenotrophic methanogens for available hydrogen (H2) and also through toxic effects on the methanogens. However, there is limited knowledge about its effects on the others members of ruminal microbiota and their metabolites. In this study, we investigated the effects of dietary nitrate inclusion on enteric methane (CH4) emission, temporal changes in ruminal microbiota, and fermentation in Holstein calves. Eighteen animals were maintained in individual pens for 45 d. Animals were randomly allocated to either a control (CTR) or nitrate (NIT, containing 15 g of calcium nitrate/kg dry matter) diets. Methane emissions were estimated using the sulfur hexafluoride (SF6) tracer method. Ruminal microbiota changes and ruminal fermentation were evaluated at 0, 4, and 8 h post-feeding. In this study, feed dry matter intake (DMI) did not differ between dietary treatments (P > 0.05). Diets containing NIT reduced CH4 emissions by 27% (g/d) and yield by 21% (g/kg DMI) compared to the CTR (P < 0.05). The pH values and total volatile fatty acids (VFA) concentration did not differ between dietary treatments (P > 0.05) but differed with time, and post-feeding (P < 0.05). Increases in the concentrations of ruminal ammonia nitrogen (NH3–N) and acetate were observed, whereas propionate decreased at 4 h post-feeding with the NIT diet (P < 0.05). Feeding the NIT diet reduced the populations of total bacteria, total methanogens, Ruminococcus albus and Ruminococcus flavefaciens, and the abundance of Succiniclasticum, Coprococcus, Treponema, Shuttlewortia, Succinivibrio, Sharpea, Pseudobutyrivibrio, and Selenomona (P < 0.05); whereas, the population of total fungi, protozoa, Fibrobacter succinogenes, Atopobium and Erysipelotrichaceae L7A_E11 increased (P < 0.05). In conclusion, feeding nitrate reduces enteric CH4 emissions and the methanogens population, whereas it decreases the propionate concentration and the abundance of bacteria involved in the succinate and acrylate pathways. Despite the altered fermentation profile and ruminal microbiota, DMI was not influenced by dietary nitrate. These findings suggest that nitrate has a predominantly direct effect on the reduction of methanogenesis and propionate synthesis.http://www.sciencedirect.com/science/article/pii/S2405654521001657Methane emissionNitrateMicrobiotaRuminal fermentation |
| spellingShingle | Abimael Ortiz-Chura José Gere Gisela Marcoppido Gustavo Depetris Silvio Cravero Claudia Faverín Cesar Pinares-Patiño Angel Cataldi María E. Cerón-Cucchi Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves Animal Nutrition Methane emission Nitrate Microbiota Ruminal fermentation |
| title | Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves |
| title_full | Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves |
| title_fullStr | Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves |
| title_full_unstemmed | Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves |
| title_short | Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves |
| title_sort | dynamics of the ruminal microbial ecosystem and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to holstein calves |
| topic | Methane emission Nitrate Microbiota Ruminal fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S2405654521001657 |
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