Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure

UHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the...

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Main Authors: Escott Carlos, Vaquero Cristian, López Carmen, Loira Iris, González Carmen, del Fresno Juan Manuel, Palomero Felipe, Suárez-Lepe José Antonio, Morata Antonio
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html
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author Escott Carlos
Vaquero Cristian
López Carmen
Loira Iris
González Carmen
del Fresno Juan Manuel
Palomero Felipe
Suárez-Lepe José Antonio
Morata Antonio
author_facet Escott Carlos
Vaquero Cristian
López Carmen
Loira Iris
González Carmen
del Fresno Juan Manuel
Palomero Felipe
Suárez-Lepe José Antonio
Morata Antonio
author_sort Escott Carlos
collection DOAJ
description UHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the applied force can disrupt bacterial and yeast cell structures, altering or breaking down proteins, polysaccharides and enzymes. This effect is not observed for low molecular weight compounds such as monomeric pigments and phenolic structures, varietal aroma precursors, fermentable sugars, etc. Treated and untreated samples can be compared using optical and atomic force microscopy. Optical microscopy images show reduction or elimination of bacteria and yeast and changes in microstructure. On the other hand, in addition to describing topography in the nanometer range, AFM can also measure particles in comparison to other techniques such as laser diffraction (LD). This work contributes to the characterization and better understanding of the effects of UHPH on grape juice for winemaking.
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spelling doaj.art-a69f20aa167d48889f8d842861b1a7652024-01-05T10:33:20ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01680200910.1051/bioconf/20236802009bioconf_oiv2023_02009Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructureEscott Carlos0Vaquero Cristian1López Carmen2Loira Iris3González Carmen4del Fresno Juan Manuel5Palomero Felipe6Suárez-Lepe José Antonio7Morata Antonio8enotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridUHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the applied force can disrupt bacterial and yeast cell structures, altering or breaking down proteins, polysaccharides and enzymes. This effect is not observed for low molecular weight compounds such as monomeric pigments and phenolic structures, varietal aroma precursors, fermentable sugars, etc. Treated and untreated samples can be compared using optical and atomic force microscopy. Optical microscopy images show reduction or elimination of bacteria and yeast and changes in microstructure. On the other hand, in addition to describing topography in the nanometer range, AFM can also measure particles in comparison to other techniques such as laser diffraction (LD). This work contributes to the characterization and better understanding of the effects of UHPH on grape juice for winemaking.https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html
spellingShingle Escott Carlos
Vaquero Cristian
López Carmen
Loira Iris
González Carmen
del Fresno Juan Manuel
Palomero Felipe
Suárez-Lepe José Antonio
Morata Antonio
Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
BIO Web of Conferences
title Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
title_full Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
title_fullStr Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
title_full_unstemmed Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
title_short Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
title_sort optical and afm microscopy of grape juices treated with uhph effects of microstructure and nanostructure
url https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html
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