Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure
UHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the...
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EDP Sciences
2023-01-01
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Series: | BIO Web of Conferences |
Online Access: | https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html |
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author | Escott Carlos Vaquero Cristian López Carmen Loira Iris González Carmen del Fresno Juan Manuel Palomero Felipe Suárez-Lepe José Antonio Morata Antonio |
author_facet | Escott Carlos Vaquero Cristian López Carmen Loira Iris González Carmen del Fresno Juan Manuel Palomero Felipe Suárez-Lepe José Antonio Morata Antonio |
author_sort | Escott Carlos |
collection | DOAJ |
description | UHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the applied force can disrupt bacterial and yeast cell structures, altering or breaking down proteins, polysaccharides and enzymes. This effect is not observed for low molecular weight compounds such as monomeric pigments and phenolic structures, varietal aroma precursors, fermentable sugars, etc. Treated and untreated samples can be compared using optical and atomic force microscopy. Optical microscopy images show reduction or elimination of bacteria and yeast and changes in microstructure. On the other hand, in addition to describing topography in the nanometer range, AFM can also measure particles in comparison to other techniques such as laser diffraction (LD). This work contributes to the characterization and better understanding of the effects of UHPH on grape juice for winemaking. |
first_indexed | 2024-03-08T16:40:23Z |
format | Article |
id | doaj.art-a69f20aa167d48889f8d842861b1a765 |
institution | Directory Open Access Journal |
issn | 2117-4458 |
language | English |
last_indexed | 2024-03-08T16:40:23Z |
publishDate | 2023-01-01 |
publisher | EDP Sciences |
record_format | Article |
series | BIO Web of Conferences |
spelling | doaj.art-a69f20aa167d48889f8d842861b1a7652024-01-05T10:33:20ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01680200910.1051/bioconf/20236802009bioconf_oiv2023_02009Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructureEscott Carlos0Vaquero Cristian1López Carmen2Loira Iris3González Carmen4del Fresno Juan Manuel5Palomero Felipe6Suárez-Lepe José Antonio7Morata Antonio8enotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridenotecUPM. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de MadridUHPH treatment of Vitis vinifera must for winemaking leads to fragmentation of colloidal particles into smaller structures. The shear and fracture forces experienced by grape juice during valve pressurization are sufficient to reduce the particle size of grape juice to below 500 nm. As a result, the applied force can disrupt bacterial and yeast cell structures, altering or breaking down proteins, polysaccharides and enzymes. This effect is not observed for low molecular weight compounds such as monomeric pigments and phenolic structures, varietal aroma precursors, fermentable sugars, etc. Treated and untreated samples can be compared using optical and atomic force microscopy. Optical microscopy images show reduction or elimination of bacteria and yeast and changes in microstructure. On the other hand, in addition to describing topography in the nanometer range, AFM can also measure particles in comparison to other techniques such as laser diffraction (LD). This work contributes to the characterization and better understanding of the effects of UHPH on grape juice for winemaking.https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html |
spellingShingle | Escott Carlos Vaquero Cristian López Carmen Loira Iris González Carmen del Fresno Juan Manuel Palomero Felipe Suárez-Lepe José Antonio Morata Antonio Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure BIO Web of Conferences |
title | Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure |
title_full | Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure |
title_fullStr | Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure |
title_full_unstemmed | Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure |
title_short | Optical and AFM microscopy of grape juices treated with UHPH: Effects of microstructure and nanostructure |
title_sort | optical and afm microscopy of grape juices treated with uhph effects of microstructure and nanostructure |
url | https://www.bio-conferences.org/articles/bioconf/full_html/2023/13/bioconf_oiv2023_02009/bioconf_oiv2023_02009.html |
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