Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs
Osteopontin (<i>OPN</i>) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and thei...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2015-08-01
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| Series: | Archives Animal Breeding |
| Online Access: | http://www.arch-anim-breed.net/58/317/2015/aab-58-317-2015.pdf |
| Summary: | Osteopontin (<i>OPN</i>) gene is a secreted phosphoprotein which appears to play a key
function in the conceptus implantation, placentation and maintenance of
pregnancy in pigs. The objectives of this study were to verify the
non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in
commercial Thai Large White pigs. A total of 320 Thai Large White sows were
genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A,
c.573T> C and c.881C> T revealed amino acid exchange
rates of p.110Ala> Thr, p.159Val> Ala and
p.262Pro> Ser, respectively, and were then segregated. These
three SNPs were significantly associated with total number born (TNB) and
number born alive (NBA) traits. No polymorphisms of the two SNP markers
(c.278A> G and c.452T> G) were observed in this study.
Moreover, the SNPs at c.425G> A and c.573T> C were
found to be in strong linkage disequilibrium. The association of <i>OPN</i> with
litter size emphasizes the importance of porcine <i>OPN</i> as a candidate gene for
reproductive traits in pig breeding. |
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| ISSN: | 0003-9438 2363-9822 |