Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro
In this study, a novel protein designated MC-2 was isolated from Momordica charantia L., and the positively regulates lipid metabolism activity was detected by the bile acid binding activity test. Then, the amino acid composition of MC-2 was analyzed, and the total content of Asp, Glu, and Lys is up...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002882 |
| _version_ | 1811188037428707328 |
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| author | Yonglin Gao Ping Liu Donghong Wang Jingmin Liu Lintong Yang Yating Kang Bing Han Jungang Yin Jing Zhu Kezhou Wang Chunmei Li |
| author_facet | Yonglin Gao Ping Liu Donghong Wang Jingmin Liu Lintong Yang Yating Kang Bing Han Jungang Yin Jing Zhu Kezhou Wang Chunmei Li |
| author_sort | Yonglin Gao |
| collection | DOAJ |
| description | In this study, a novel protein designated MC-2 was isolated from Momordica charantia L., and the positively regulates lipid metabolism activity was detected by the bile acid binding activity test. Then, the amino acid composition of MC-2 was analyzed, and the total content of Asp, Glu, and Lys is up to 38.96 g/100 g in MC-2. Furtherly, its lipid metabolism activity were explored. In vitro, MC-2 ameliorated FFA-induced HepG2 steatosis phenotypes and intracellular triglycerides level with the promotion of bile acids secretion. In vivo, MC-2 was able to protect rats against NAFLD-induced in response to a high-fat diet through a manner associated with the regulation of inflammatory cytokines and oxidative stress-related factors. The hepatoprotective activity of MC-2 was closely tied to improvements CPT1A, FATP2, ACSL1, and PPAR-α expressions. Together, these data highlight the promise of MC-2 (rich in Asp, Glu, and Lys) as a candidate functional food ingredient. |
| first_indexed | 2024-04-11T14:13:47Z |
| format | Article |
| id | doaj.art-a6a85081d9bf4cbaa728aea81a1fb648 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T14:13:47Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-a6a85081d9bf4cbaa728aea81a1fb6482022-12-22T04:19:37ZengElsevierJournal of Functional Foods1756-46462022-09-0196105218Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitroYonglin Gao0Ping Liu1Donghong Wang2Jingmin Liu3Lintong Yang4Yating Kang5Bing Han6Jungang Yin7Jing Zhu8Kezhou Wang9Chunmei Li10College of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaLuye Pharma Group Ltd., Yantai 264003, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of experimental animals, Shandong First Medical University, Jinan 250002, ChinaCollege of experimental animals, Shandong First Medical University, Jinan 250002, ChinaSchool of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, China; Corresponding author.In this study, a novel protein designated MC-2 was isolated from Momordica charantia L., and the positively regulates lipid metabolism activity was detected by the bile acid binding activity test. Then, the amino acid composition of MC-2 was analyzed, and the total content of Asp, Glu, and Lys is up to 38.96 g/100 g in MC-2. Furtherly, its lipid metabolism activity were explored. In vitro, MC-2 ameliorated FFA-induced HepG2 steatosis phenotypes and intracellular triglycerides level with the promotion of bile acids secretion. In vivo, MC-2 was able to protect rats against NAFLD-induced in response to a high-fat diet through a manner associated with the regulation of inflammatory cytokines and oxidative stress-related factors. The hepatoprotective activity of MC-2 was closely tied to improvements CPT1A, FATP2, ACSL1, and PPAR-α expressions. Together, these data highlight the promise of MC-2 (rich in Asp, Glu, and Lys) as a candidate functional food ingredient.http://www.sciencedirect.com/science/article/pii/S1756464622002882Momordica charantia L.Lipid metabolismBile acid binding capacityNonalcoholic fatty liver disease (NAFLD)Anti-inflammatoryROS generation |
| spellingShingle | Yonglin Gao Ping Liu Donghong Wang Jingmin Liu Lintong Yang Yating Kang Bing Han Jungang Yin Jing Zhu Kezhou Wang Chunmei Li Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro Journal of Functional Foods Momordica charantia L. Lipid metabolism Bile acid binding capacity Nonalcoholic fatty liver disease (NAFLD) Anti-inflammatory ROS generation |
| title | Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro |
| title_full | Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro |
| title_fullStr | Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro |
| title_full_unstemmed | Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro |
| title_short | Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro |
| title_sort | isolation and characterization of a novel protein from momordica charantia l positively regulates lipid metabolism activity in vivo and in vitro |
| topic | Momordica charantia L. Lipid metabolism Bile acid binding capacity Nonalcoholic fatty liver disease (NAFLD) Anti-inflammatory ROS generation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002882 |
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