The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth
Abstract Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12497 |
| _version_ | 1827398903134158848 |
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| author | Taketo Inoue Sayumi Taguchi Mikiko Uemura Yoshiko Tsujimoto Kana Kokunai Kayoe Ikawa Yoshiki Yamashita |
| author_facet | Taketo Inoue Sayumi Taguchi Mikiko Uemura Yoshiko Tsujimoto Kana Kokunai Kayoe Ikawa Yoshiki Yamashita |
| author_sort | Taketo Inoue |
| collection | DOAJ |
| description | Abstract Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed in IVF‐derived zygotes. The central coordinates of the mPN, fPN, and NPBs were noted at multiple timepoints. The migration distance of NPBs between two sequential images was measured to calculate NPB migration speed. Results The NPB migration speeds in mPN and fPN were significantly faster in euploid zygotes than in aneuploid zygotes. In multivariate logistic analysis, NPB migration speed in mPN and the female age were associated with euploidy. The NPB migration speeds in mPN and fPN were also significantly faster in zygotes that led to LB than in zygotes that led to no pregnancy. In a receiver operating characteristic curve analysis of LB by NPB migration speed in mPN, the cut‐off value was 3.74 μm/h (AUC: 0.825, 95%CI: 0.688–0.963). When the zygotes were categorized by this cut‐off value, there were significantly more LBs in zygotes with migration speed ≥ the cut‐off (78.9% vs. 21.1%). Conclusions Zygotes with quickly migrating NPBs demonstrated the developmental potential to become a baby. |
| first_indexed | 2024-03-08T19:35:37Z |
| format | Article |
| id | doaj.art-a6ab4924569d4d17a3f1e896c84efb39 |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-03-08T19:35:37Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-a6ab4924569d4d17a3f1e896c84efb392023-12-26T04:30:45ZengWileyReproductive Medicine and Biology1445-57811447-05782023-01-01221n/an/a10.1002/rmb2.12497The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birthTaketo Inoue0Sayumi Taguchi1Mikiko Uemura2Yoshiko Tsujimoto3Kana Kokunai4Kayoe Ikawa5Yoshiki Yamashita6Umeda Fertility Clinic Osaka JapanUmeda Fertility Clinic Osaka JapanDepartment of Rehabilitation, Faculty of Health Science Kansai University of Welfare Sciences Osaka JapanUmeda Fertility Clinic Osaka JapanUmeda Fertility Clinic Osaka JapanUmeda Fertility Clinic Osaka JapanUmeda Fertility Clinic Osaka JapanAbstract Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed in IVF‐derived zygotes. The central coordinates of the mPN, fPN, and NPBs were noted at multiple timepoints. The migration distance of NPBs between two sequential images was measured to calculate NPB migration speed. Results The NPB migration speeds in mPN and fPN were significantly faster in euploid zygotes than in aneuploid zygotes. In multivariate logistic analysis, NPB migration speed in mPN and the female age were associated with euploidy. The NPB migration speeds in mPN and fPN were also significantly faster in zygotes that led to LB than in zygotes that led to no pregnancy. In a receiver operating characteristic curve analysis of LB by NPB migration speed in mPN, the cut‐off value was 3.74 μm/h (AUC: 0.825, 95%CI: 0.688–0.963). When the zygotes were categorized by this cut‐off value, there were significantly more LBs in zygotes with migration speed ≥ the cut‐off (78.9% vs. 21.1%). Conclusions Zygotes with quickly migrating NPBs demonstrated the developmental potential to become a baby.https://doi.org/10.1002/rmb2.12497embryo developmentlive birthnucleolus precursor bodypronucleizygote |
| spellingShingle | Taketo Inoue Sayumi Taguchi Mikiko Uemura Yoshiko Tsujimoto Kana Kokunai Kayoe Ikawa Yoshiki Yamashita The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth Reproductive Medicine and Biology embryo development live birth nucleolus precursor body pronuclei zygote |
| title | The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth |
| title_full | The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth |
| title_fullStr | The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth |
| title_full_unstemmed | The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth |
| title_short | The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth |
| title_sort | migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization derived human embryo ploidy status and live birth |
| topic | embryo development live birth nucleolus precursor body pronuclei zygote |
| url | https://doi.org/10.1002/rmb2.12497 |
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