Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion
Background The effects of FBXO43 on hepatocellular carcinoma (HCC) and its clinical significance have not yet been determined. This study aims to determine the clinical significance of FBXO43 in HCC and its impact on the biological functions of HCC cells. Methods Data from TCGA database were downloa...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2023-05-01
|
Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/15373.pdf |
_version_ | 1797417594830979072 |
---|---|
author | Rulan Ma Wenbo Liu Tuanhe Sun Chengxue Dang Kang Li |
author_facet | Rulan Ma Wenbo Liu Tuanhe Sun Chengxue Dang Kang Li |
author_sort | Rulan Ma |
collection | DOAJ |
description | Background The effects of FBXO43 on hepatocellular carcinoma (HCC) and its clinical significance have not yet been determined. This study aims to determine the clinical significance of FBXO43 in HCC and its impact on the biological functions of HCC cells. Methods Data from TCGA database were downloaded to investigate the expression of FBXO43 in HCC and its correlation with prognosis and immune infiltration. Immunohistochemical staining images of FBXO43 in HCC were acquired from the HPA website. HCC cells (BEL-7404 and SMMC-7721) were transfected with the lentivirus targeting FBXO43 to decrease FBXO43 expression in HCC cells. Western blotting assay was conducted to evaluate the expression level of FBXO43 protein. MTT assay was used to detect the proliferation of HCC cells. The migration and invasion of HCC cells were investigated by performing scratch wound-healing and Transwell invasion assays, respectively. Results In comparison to normal tissues, FBXO43 is overexpressed in HCC tissue, and high FBXO43 expression is linked to late T stage, TNM stage and tumor grade. Elevated FBXO43 expression is a risk factor for HCC. In patients with high FBXO43 expression, the overall survival, disease-specific survival, progression-free survival and disease-free survival are poorer. The proliferation, migration and invasion of HCC cells are significantly attenuated in FBXO43 knockdown cells. Also, TCGA data analysis reveals that FBXO43 exhibits a positive correlation with immunosuppression of HCC. Conclusion FBXO43 is overexpressed in HCC, and is linked to late tumor stage, worse prognosis and tumor immunosuppression. FBXO43 knockdown restrains the proliferation, migration and invasion of HCC. |
first_indexed | 2024-03-09T06:22:04Z |
format | Article |
id | doaj.art-a6ab87cce1bb4e1fae270c26bed0efdc |
institution | Directory Open Access Journal |
issn | 2167-8359 |
language | English |
last_indexed | 2024-03-09T06:22:04Z |
publishDate | 2023-05-01 |
publisher | PeerJ Inc. |
record_format | Article |
series | PeerJ |
spelling | doaj.art-a6ab87cce1bb4e1fae270c26bed0efdc2023-12-03T11:38:48ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1537310.7717/peerj.15373Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasionRulan Ma0Wenbo Liu1Tuanhe Sun2Chengxue Dang3Kang Li4Department of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Plastic and Cosmetic Maxillofacial Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shannxi, ChinaDepartment of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Surgical Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaBackground The effects of FBXO43 on hepatocellular carcinoma (HCC) and its clinical significance have not yet been determined. This study aims to determine the clinical significance of FBXO43 in HCC and its impact on the biological functions of HCC cells. Methods Data from TCGA database were downloaded to investigate the expression of FBXO43 in HCC and its correlation with prognosis and immune infiltration. Immunohistochemical staining images of FBXO43 in HCC were acquired from the HPA website. HCC cells (BEL-7404 and SMMC-7721) were transfected with the lentivirus targeting FBXO43 to decrease FBXO43 expression in HCC cells. Western blotting assay was conducted to evaluate the expression level of FBXO43 protein. MTT assay was used to detect the proliferation of HCC cells. The migration and invasion of HCC cells were investigated by performing scratch wound-healing and Transwell invasion assays, respectively. Results In comparison to normal tissues, FBXO43 is overexpressed in HCC tissue, and high FBXO43 expression is linked to late T stage, TNM stage and tumor grade. Elevated FBXO43 expression is a risk factor for HCC. In patients with high FBXO43 expression, the overall survival, disease-specific survival, progression-free survival and disease-free survival are poorer. The proliferation, migration and invasion of HCC cells are significantly attenuated in FBXO43 knockdown cells. Also, TCGA data analysis reveals that FBXO43 exhibits a positive correlation with immunosuppression of HCC. Conclusion FBXO43 is overexpressed in HCC, and is linked to late tumor stage, worse prognosis and tumor immunosuppression. FBXO43 knockdown restrains the proliferation, migration and invasion of HCC.https://peerj.com/articles/15373.pdfFBXO43Hepatocellular carcinomaPrognosisImmune infiltrationTumor growth |
spellingShingle | Rulan Ma Wenbo Liu Tuanhe Sun Chengxue Dang Kang Li Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion PeerJ FBXO43 Hepatocellular carcinoma Prognosis Immune infiltration Tumor growth |
title | Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion |
title_full | Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion |
title_fullStr | Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion |
title_full_unstemmed | Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion |
title_short | Clinical significance of FBXO43 in hepatocellular carcinoma and its impact on tumor cell proliferation, migration and invasion |
title_sort | clinical significance of fbxo43 in hepatocellular carcinoma and its impact on tumor cell proliferation migration and invasion |
topic | FBXO43 Hepatocellular carcinoma Prognosis Immune infiltration Tumor growth |
url | https://peerj.com/articles/15373.pdf |
work_keys_str_mv | AT rulanma clinicalsignificanceoffbxo43inhepatocellularcarcinomaanditsimpactontumorcellproliferationmigrationandinvasion AT wenboliu clinicalsignificanceoffbxo43inhepatocellularcarcinomaanditsimpactontumorcellproliferationmigrationandinvasion AT tuanhesun clinicalsignificanceoffbxo43inhepatocellularcarcinomaanditsimpactontumorcellproliferationmigrationandinvasion AT chengxuedang clinicalsignificanceoffbxo43inhepatocellularcarcinomaanditsimpactontumorcellproliferationmigrationandinvasion AT kangli clinicalsignificanceoffbxo43inhepatocellularcarcinomaanditsimpactontumorcellproliferationmigrationandinvasion |