Inter-laboratory comparison of methods to measure androstenone in pork fat
Today, different analytical methods are used by different laboratories to quantify androstenone in fat tissue. This study shows the comparison of methods used routinely in different laboratories for androstenone quantification: Time-resolved fluoroimmunoassay in Norwegian School of Veterinary Scienc...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731111000553 |
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| author | S. Ampuero Kragten B. Verkuylen H. Dahlmans M. Hortos J.A. Garcia-Regueiro E. Dahl O. Andresen H. Feitsma P.K. Mathur B. Harlizius |
| author_facet | S. Ampuero Kragten B. Verkuylen H. Dahlmans M. Hortos J.A. Garcia-Regueiro E. Dahl O. Andresen H. Feitsma P.K. Mathur B. Harlizius |
| author_sort | S. Ampuero Kragten |
| collection | DOAJ |
| description | Today, different analytical methods are used by different laboratories to quantify androstenone in fat tissue. This study shows the comparison of methods used routinely in different laboratories for androstenone quantification: Time-resolved fluoroimmunoassay in Norwegian School of Veterinary Science (NSVS; Norway), gas chromatography coupled to mass spectrometry in Co-operative Central Laboratory (CCL; The Netherlands) and in Institut de Recerca i Tecnologia Agroalimentàries (IRTA; Spain), and high-pressure liquid chromatography in Agroscope Liebefeld-Posieux Research Station (ALP; Switzerland). In a first trial, a set of adipose tissue (AT) samples from 53 entire males was sent to CCL, IRTA and NSVS for determination of androstenone concentration. The average androstenone concentration (s.d.) was 2.47 (2.10) μg/g at NSVS, 1.31 (0.98) μg/g at CCL and 0.62 (0.52) μg/g at IRTA. Despite the large differences in absolute values, inter-laboratory correlations were high, ranging from 0.82 to 0.92. A closer look showed differences in the preparation step. Indeed, different matrices were used for the analysis: pure fat at NSVS, melted fat at CCL and AT at IRTA. A second trial was organised in order to circumvent the differences in sample preparation. Back fat samples from 10 entire males were lyophilised at the ALP labortary in Switzerland and were sent to the other laboratories for androstenone concentration measurement. The average concentration (s.d.) of androstenone in the freeze-dried AT samples was 0.87 (0.52), 1.03 (0.55), 0.84 (0.46) and 0.99 (0.67) μg/g at NSVS, CCL, IRTA and ALP, respectively, and the pairwise correlations between laboratories ranged from 0.92 to 0.97. Thus, this study shows the influence of the different sample preparation protocols, leading to major differences in the results, although still allowing high inter-laboratory correlations. The results further highlight the need for method standardisation and inter-laboratory ring tests for the determination of androstenone. This standardisation is especially relevant when deriving thresholds of consumer acceptance, whereas the ranking of animals for breeding purposes will be less affected due to the high correlations between methods. |
| first_indexed | 2024-12-19T10:39:46Z |
| format | Article |
| id | doaj.art-a6ac2c14ff2f4ca8a00aa8bdcb109868 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-19T10:39:46Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier |
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| series | Animal |
| spelling | doaj.art-a6ac2c14ff2f4ca8a00aa8bdcb1098682022-12-21T20:25:29ZengElsevierAnimal1751-73112011-01-0151016341642Inter-laboratory comparison of methods to measure androstenone in pork fatS. Ampuero Kragten0B. Verkuylen1H. Dahlmans2M. Hortos3J.A. Garcia-Regueiro4E. Dahl5O. Andresen6H. Feitsma7P.K. Mathur8B. Harlizius9Chemical Department, Agroscope Liebefeld-Posieux Research Station ALP, Tioleyre 4, 1725 Posieux, SwitzerlandInstrumental Analysis, Co-operative Central Laboratory CCL, PO Box 107, 5460 AC Veghel, The NetherlandsInstrumental Analysis, Co-operative Central Laboratory CCL, PO Box 107, 5460 AC Veghel, The NetherlandsFunctionality and Nutrition, Institut de Recerca i Tecnologia Agroalimentàries IRTA, Food Technology, Finca Camps i Armet 17121 Monells, SpainFunctionality and Nutrition, Institut de Recerca i Tecnologia Agroalimentàries IRTA, Food Technology, Finca Camps i Armet 17121 Monells, SpainDepartment of Production Animal Clinical Sciences, Norwegian School of Veterinary Sciences, PO Box 8146 Dep, 0033 Oslo, NorwayDepartment of Production Animal Clinical Sciences, Norwegian School of Veterinary Sciences, PO Box 8146 Dep, 0033 Oslo, NorwayResearch and Development Department, Institute for Pig Genetics B.V., PO Box 43, 6640 AA Beuningen, The NetherlandsResearch and Development Department, Institute for Pig Genetics B.V., PO Box 43, 6640 AA Beuningen, The NetherlandsResearch and Development Department, Institute for Pig Genetics B.V., PO Box 43, 6640 AA Beuningen, The NetherlandsToday, different analytical methods are used by different laboratories to quantify androstenone in fat tissue. This study shows the comparison of methods used routinely in different laboratories for androstenone quantification: Time-resolved fluoroimmunoassay in Norwegian School of Veterinary Science (NSVS; Norway), gas chromatography coupled to mass spectrometry in Co-operative Central Laboratory (CCL; The Netherlands) and in Institut de Recerca i Tecnologia Agroalimentàries (IRTA; Spain), and high-pressure liquid chromatography in Agroscope Liebefeld-Posieux Research Station (ALP; Switzerland). In a first trial, a set of adipose tissue (AT) samples from 53 entire males was sent to CCL, IRTA and NSVS for determination of androstenone concentration. The average androstenone concentration (s.d.) was 2.47 (2.10) μg/g at NSVS, 1.31 (0.98) μg/g at CCL and 0.62 (0.52) μg/g at IRTA. Despite the large differences in absolute values, inter-laboratory correlations were high, ranging from 0.82 to 0.92. A closer look showed differences in the preparation step. Indeed, different matrices were used for the analysis: pure fat at NSVS, melted fat at CCL and AT at IRTA. A second trial was organised in order to circumvent the differences in sample preparation. Back fat samples from 10 entire males were lyophilised at the ALP labortary in Switzerland and were sent to the other laboratories for androstenone concentration measurement. The average concentration (s.d.) of androstenone in the freeze-dried AT samples was 0.87 (0.52), 1.03 (0.55), 0.84 (0.46) and 0.99 (0.67) μg/g at NSVS, CCL, IRTA and ALP, respectively, and the pairwise correlations between laboratories ranged from 0.92 to 0.97. Thus, this study shows the influence of the different sample preparation protocols, leading to major differences in the results, although still allowing high inter-laboratory correlations. The results further highlight the need for method standardisation and inter-laboratory ring tests for the determination of androstenone. This standardisation is especially relevant when deriving thresholds of consumer acceptance, whereas the ranking of animals for breeding purposes will be less affected due to the high correlations between methods.http://www.sciencedirect.com/science/article/pii/S1751731111000553boar taintandrostenone analysispork |
| spellingShingle | S. Ampuero Kragten B. Verkuylen H. Dahlmans M. Hortos J.A. Garcia-Regueiro E. Dahl O. Andresen H. Feitsma P.K. Mathur B. Harlizius Inter-laboratory comparison of methods to measure androstenone in pork fat Animal boar taint androstenone analysis pork |
| title | Inter-laboratory comparison of methods to measure androstenone in pork fat |
| title_full | Inter-laboratory comparison of methods to measure androstenone in pork fat |
| title_fullStr | Inter-laboratory comparison of methods to measure androstenone in pork fat |
| title_full_unstemmed | Inter-laboratory comparison of methods to measure androstenone in pork fat |
| title_short | Inter-laboratory comparison of methods to measure androstenone in pork fat |
| title_sort | inter laboratory comparison of methods to measure androstenone in pork fat |
| topic | boar taint androstenone analysis pork |
| url | http://www.sciencedirect.com/science/article/pii/S1751731111000553 |
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