Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa
This cross-sectional study was conducted at the slaughterhouses/slabs of Oudalan and Ouagadougou in Burkina Faso, between August and September 2013. It aimed at determining the prevalence of bovine tuberculosis (bTB) suggestive lesions in slaughtered cattle carcasses and to identify and characterize...
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1378 |
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| author | Estelle Kanyala Yassir Adam Shuaib Norbert Georg Schwarz Sönke Andres Elvira Richter Bernard Sawadogo Mamadou Sawadogo Minoungou Germaine Ouattara Lassina Sven Poppert Hagen Frickmann |
| author_facet | Estelle Kanyala Yassir Adam Shuaib Norbert Georg Schwarz Sönke Andres Elvira Richter Bernard Sawadogo Mamadou Sawadogo Minoungou Germaine Ouattara Lassina Sven Poppert Hagen Frickmann |
| author_sort | Estelle Kanyala |
| collection | DOAJ |
| description | This cross-sectional study was conducted at the slaughterhouses/slabs of Oudalan and Ouagadougou in Burkina Faso, between August and September 2013. It aimed at determining the prevalence of bovine tuberculosis (bTB) suggestive lesions in slaughtered cattle carcasses and to identify and characterize the mycobacteria isolated from these lesions. A thorough postmortem examination was conducted on carcasses of a total of 2165 randomly selected cattle. The overall prevalence of bTB suggestive lesions was 2.7% (58/2165; 95% CI 2.1–3.5%). Due to the low number of positive samples, data were descriptively presented. The lesions were either observed localized in one or a few organs or generalized (i.e., miliary bTB) in 96.6% (<i>n</i> = 57) and 3.4% (<i>n</i> = 2), respectively. The identified mycobacteria were <i>M. bovis</i> (44.4%, <i>n</i> = 20), <i>M. fortuitum</i> (8.9%, <i>n</i> = 4), <i>M. elephantis</i> (6.7%, <i>n</i> = 3), <i>M. brumae</i> (4.4%, <i>n</i> = 2), <i>M. avium</i> (2.2%, <i>n</i> = 1), <i>M. asiaticum</i> (2.2%, <i>n</i> = 1), <i>M. terrae</i> (2.2%, <i>n</i> = 1), and unknown non-tuberculous mycobacteria (NTM) (11.1%, <i>n</i> = 5). Moreover, eight mixed cultures with more than one <i>Mycobacterium</i> species growing were also observed, of which three were <i>M. bovis</i> and <i>M. fortuitum</i> and three were <i>M. bovis</i> and <i>M. elephantis</i>. In conclusion, <i>M. bovis</i> is the predominant causative agent of mycobacterial infections in the study area. Our study has identified a base to broaden the epidemiological knowledge on zoonotic transmission of mycobacteria in Burkina Faso by future studies investigating further samples from humans and animals, including wild animals employing molecular techniques. |
| first_indexed | 2024-03-09T10:14:37Z |
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| language | English |
| last_indexed | 2024-03-09T10:14:37Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-a6b2fbc37fa44a0e955b2197b4532d5a2023-12-01T22:28:29ZengMDPI AGMicroorganisms2076-26072022-07-01107137810.3390/microorganisms10071378Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West AfricaEstelle Kanyala0Yassir Adam Shuaib1Norbert Georg Schwarz2Sönke Andres3Elvira Richter4Bernard Sawadogo5Mamadou Sawadogo6Minoungou Germaine7Ouattara Lassina8Sven Poppert9Hagen Frickmann10Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso BP 390, Burkina FasoCollege of Veterinary Medicine, Sudan University of Science and Technology, P.O. Box 204, Khartoum North 13321, SudanIndependent Researcher, 67459 Böhl-Iggelheim, GermanyNational Reference Laboratory for Mycobacteria, Research Center Borstel, 23845 Borstel, GermanyTuberculosis Laboratory, Laboratory Limbach, 69126 Heidelberg, GermanyWest Africa Francophone, African Field Epidemiology Network (AFENET), Ouagadougou 01 BP 364, Burkina FasoLaboratory of Biochemistry, Health Sciences Training and Research Unit, University of Ouagadougou, Ouagadougou BP 7021, Burkina FasoLaboratoire National d’Elevage (LNE), Ouagadougou BP 907, Burkina FasoDirection Générale des Services Vétérinaires (DGSv), Ouagadougou 01 BP 364, Burkina FasoBernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, GermanyThis cross-sectional study was conducted at the slaughterhouses/slabs of Oudalan and Ouagadougou in Burkina Faso, between August and September 2013. It aimed at determining the prevalence of bovine tuberculosis (bTB) suggestive lesions in slaughtered cattle carcasses and to identify and characterize the mycobacteria isolated from these lesions. A thorough postmortem examination was conducted on carcasses of a total of 2165 randomly selected cattle. The overall prevalence of bTB suggestive lesions was 2.7% (58/2165; 95% CI 2.1–3.5%). Due to the low number of positive samples, data were descriptively presented. The lesions were either observed localized in one or a few organs or generalized (i.e., miliary bTB) in 96.6% (<i>n</i> = 57) and 3.4% (<i>n</i> = 2), respectively. The identified mycobacteria were <i>M. bovis</i> (44.4%, <i>n</i> = 20), <i>M. fortuitum</i> (8.9%, <i>n</i> = 4), <i>M. elephantis</i> (6.7%, <i>n</i> = 3), <i>M. brumae</i> (4.4%, <i>n</i> = 2), <i>M. avium</i> (2.2%, <i>n</i> = 1), <i>M. asiaticum</i> (2.2%, <i>n</i> = 1), <i>M. terrae</i> (2.2%, <i>n</i> = 1), and unknown non-tuberculous mycobacteria (NTM) (11.1%, <i>n</i> = 5). Moreover, eight mixed cultures with more than one <i>Mycobacterium</i> species growing were also observed, of which three were <i>M. bovis</i> and <i>M. fortuitum</i> and three were <i>M. bovis</i> and <i>M. elephantis</i>. In conclusion, <i>M. bovis</i> is the predominant causative agent of mycobacterial infections in the study area. Our study has identified a base to broaden the epidemiological knowledge on zoonotic transmission of mycobacteria in Burkina Faso by future studies investigating further samples from humans and animals, including wild animals employing molecular techniques.https://www.mdpi.com/2076-2607/10/7/1378Burkina Fasocattle<i>Mycobacterium bovis</i>tuberculosis |
| spellingShingle | Estelle Kanyala Yassir Adam Shuaib Norbert Georg Schwarz Sönke Andres Elvira Richter Bernard Sawadogo Mamadou Sawadogo Minoungou Germaine Ouattara Lassina Sven Poppert Hagen Frickmann Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa Microorganisms Burkina Faso cattle <i>Mycobacterium bovis</i> tuberculosis |
| title | Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa |
| title_full | Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa |
| title_fullStr | Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa |
| title_full_unstemmed | Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa |
| title_short | Prevalence and Molecular Characterization of <i>Mycobacterium bovis</i> in Slaughtered Cattle Carcasses in Burkina Faso; West Africa |
| title_sort | prevalence and molecular characterization of i mycobacterium bovis i in slaughtered cattle carcasses in burkina faso west africa |
| topic | Burkina Faso cattle <i>Mycobacterium bovis</i> tuberculosis |
| url | https://www.mdpi.com/2076-2607/10/7/1378 |
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