Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation
<i>Gypsophila paniculata</i> is one of the most popular cut flowers in the world whose major cultivars are blooming white. As is well known, blue flowers could be generated via the overexpression of the gene encoding flavonoid 3′5′-hydroxylase (F3′5′H) in species that naturally lack it....
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MDPI AG
2023-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/3/321 |
| _version_ | 1827749519974989824 |
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| author | Chunlian Jin Dan Sun Lulin Ma Xijun Mo Chunmei Yang Fan Li |
| author_facet | Chunlian Jin Dan Sun Lulin Ma Xijun Mo Chunmei Yang Fan Li |
| author_sort | Chunlian Jin |
| collection | DOAJ |
| description | <i>Gypsophila paniculata</i> is one of the most popular cut flowers in the world whose major cultivars are blooming white. As is well known, blue flowers could be generated via the overexpression of the gene encoding flavonoid 3′5′-hydroxylase (F3′5′H) in species that naturally lack it. In this study, we established the regeneration and a genetic transformation system for the commercial cultivar ‘YX4’ of <i>G. paniculata</i> and introduced the <i>F3′5′H</i> of <i>Platycodon grandiflorus</i> (<i>PgF3′5′H</i>) successfully into ‘YX4’ using the established protocol. A total of 281 hygromycin (Hyg)-resistant plantlets were obtained, and 38 of them were polymerase chain reaction (PCR) positive, indicating a 13.5% transformation efficiency. Shoot apex without meristem was more suitable for explant due to its high regeneration capacity, and the supplement of thidiazuron (TDZ) provided the most efficient promotion of adventitious bud induction, whereas the supplement of 6-Benzyladenine (6-BA) and 1-naphthaleneacetic acid (NAA) did not affect much. Additionally, the combination of 1 day (d) pre-culture, 5 d co-culture, 10 min infection, 30 mg·L<sup>−1</sup> additional acetosyringone (AS) supplement, and 10 mg·L<sup>−1</sup> Hyg selection formed the optimized system for ‘YX4’ transformation. This reliable and efficient agrobacterium-mediated transformation of the valuable commercial cultivar ‘YX4’ will contribute not only to the creation and improvement of <i>G. paniculata</i> cultivars, but also to the function research of genes associated with important ornamental traits. |
| first_indexed | 2024-03-11T06:28:19Z |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T06:28:19Z |
| publishDate | 2023-03-01 |
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| series | Horticulturae |
| spelling | doaj.art-a6b5e5cc1484449f83f3f9fbc128229e2023-11-17T11:24:03ZengMDPI AGHorticulturae2311-75242023-03-019332110.3390/horticulturae9030321Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated TransformationChunlian Jin0Dan Sun1Lulin Ma2Xijun Mo3Chunmei Yang4Fan Li5Key Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaKey Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaKey Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaKey Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaKey Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaKey Laboratory for Flower Breeding of Yunnan Province, National Engineering Research Center for Ornamental Horticulture, Floriculture Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China<i>Gypsophila paniculata</i> is one of the most popular cut flowers in the world whose major cultivars are blooming white. As is well known, blue flowers could be generated via the overexpression of the gene encoding flavonoid 3′5′-hydroxylase (F3′5′H) in species that naturally lack it. In this study, we established the regeneration and a genetic transformation system for the commercial cultivar ‘YX4’ of <i>G. paniculata</i> and introduced the <i>F3′5′H</i> of <i>Platycodon grandiflorus</i> (<i>PgF3′5′H</i>) successfully into ‘YX4’ using the established protocol. A total of 281 hygromycin (Hyg)-resistant plantlets were obtained, and 38 of them were polymerase chain reaction (PCR) positive, indicating a 13.5% transformation efficiency. Shoot apex without meristem was more suitable for explant due to its high regeneration capacity, and the supplement of thidiazuron (TDZ) provided the most efficient promotion of adventitious bud induction, whereas the supplement of 6-Benzyladenine (6-BA) and 1-naphthaleneacetic acid (NAA) did not affect much. Additionally, the combination of 1 day (d) pre-culture, 5 d co-culture, 10 min infection, 30 mg·L<sup>−1</sup> additional acetosyringone (AS) supplement, and 10 mg·L<sup>−1</sup> Hyg selection formed the optimized system for ‘YX4’ transformation. This reliable and efficient agrobacterium-mediated transformation of the valuable commercial cultivar ‘YX4’ will contribute not only to the creation and improvement of <i>G. paniculata</i> cultivars, but also to the function research of genes associated with important ornamental traits.https://www.mdpi.com/2311-7524/9/3/321<i>Gypsophila paniculata</i>regenerationgenetic transformationcommercial cultivar<i>PgF3′5′H</i> |
| spellingShingle | Chunlian Jin Dan Sun Lulin Ma Xijun Mo Chunmei Yang Fan Li Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation Horticulturae <i>Gypsophila paniculata</i> regeneration genetic transformation commercial cultivar <i>PgF3′5′H</i> |
| title | Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation |
| title_full | Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation |
| title_fullStr | Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation |
| title_full_unstemmed | Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation |
| title_short | Ectopic Expression of <i>PgF3′5′H</i> in Commercial <i>Gypsophila paniculata</i> Cultivar through Optimized Agrobacterium-Mediated Transformation |
| title_sort | ectopic expression of i pgf3 5 h i in commercial i gypsophila paniculata i cultivar through optimized agrobacterium mediated transformation |
| topic | <i>Gypsophila paniculata</i> regeneration genetic transformation commercial cultivar <i>PgF3′5′H</i> |
| url | https://www.mdpi.com/2311-7524/9/3/321 |
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