Electrical Stimulation in Cartilage Tissue Engineering
Electrical stimulation (ES) has been frequently used in different biomedical applications both in vitro and in vivo. Numerous studies have demonstrated positive effects of ES on cellular functions, including metabolism, proliferation, and differentiation. The application of ES to cartilage tissue fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/4/454 |
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| author | Raminta Vaiciuleviciute Ilona Uzieliene Paulius Bernotas Vitalij Novickij Aidas Alaburda Eiva Bernotiene |
| author_facet | Raminta Vaiciuleviciute Ilona Uzieliene Paulius Bernotas Vitalij Novickij Aidas Alaburda Eiva Bernotiene |
| author_sort | Raminta Vaiciuleviciute |
| collection | DOAJ |
| description | Electrical stimulation (ES) has been frequently used in different biomedical applications both in vitro and in vivo. Numerous studies have demonstrated positive effects of ES on cellular functions, including metabolism, proliferation, and differentiation. The application of ES to cartilage tissue for increasing extracellular matrix formation is of interest, as cartilage is not able to restore its lesions owing to its avascular nature and lack of cells. Various ES approaches have been used to stimulate chondrogenic differentiation in chondrocytes and stem cells; however, there is a huge gap in systematizing ES protocols used for chondrogenic differentiation of cells. This review focuses on the application of ES for chondrocyte and mesenchymal stem cell chondrogenesis for cartilage tissue regeneration. The effects of different types of ES on cellular functions and chondrogenic differentiation are reviewed, systematically providing ES protocols and their advantageous effects. Moreover, cartilage 3D modeling using cells in scaffolds/hydrogels under ES are observed, and recommendations on reporting about the use of ES in different studies are provided to ensure adequate consolidation of knowledge in the area of ES. This review brings novel insights into the further application of ES in in vitro studies, which are promising for further cartilage repair techniques. |
| first_indexed | 2024-03-11T05:14:15Z |
| format | Article |
| id | doaj.art-a6b60da4da704149b6927ece72c3ba34 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T05:14:15Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-a6b60da4da704149b6927ece72c3ba342023-11-17T18:22:19ZengMDPI AGBioengineering2306-53542023-04-0110445410.3390/bioengineering10040454Electrical Stimulation in Cartilage Tissue EngineeringRaminta Vaiciuleviciute0Ilona Uzieliene1Paulius Bernotas2Vitalij Novickij3Aidas Alaburda4Eiva Bernotiene5Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu g. 5, 08410 Vilnius, LithuaniaDepartment of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu g. 5, 08410 Vilnius, LithuaniaDepartment of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu g. 5, 08410 Vilnius, LithuaniaDepartment of Immunology, State Research Institute Centre for Innovative Medicine, Santariškių g. 5, 08410 Vilnius, LithuaniaLife Sciences Center, Institute of Biosciences, Vilnius University, Sauletekio al. 7, 10257 Vilnius, LithuaniaDepartment of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Santariskiu g. 5, 08410 Vilnius, LithuaniaElectrical stimulation (ES) has been frequently used in different biomedical applications both in vitro and in vivo. Numerous studies have demonstrated positive effects of ES on cellular functions, including metabolism, proliferation, and differentiation. The application of ES to cartilage tissue for increasing extracellular matrix formation is of interest, as cartilage is not able to restore its lesions owing to its avascular nature and lack of cells. Various ES approaches have been used to stimulate chondrogenic differentiation in chondrocytes and stem cells; however, there is a huge gap in systematizing ES protocols used for chondrogenic differentiation of cells. This review focuses on the application of ES for chondrocyte and mesenchymal stem cell chondrogenesis for cartilage tissue regeneration. The effects of different types of ES on cellular functions and chondrogenic differentiation are reviewed, systematically providing ES protocols and their advantageous effects. Moreover, cartilage 3D modeling using cells in scaffolds/hydrogels under ES are observed, and recommendations on reporting about the use of ES in different studies are provided to ensure adequate consolidation of knowledge in the area of ES. This review brings novel insights into the further application of ES in in vitro studies, which are promising for further cartilage repair techniques.https://www.mdpi.com/2306-5354/10/4/454cartilageosteoarthritischondrogenesiselectrical stimulationmesenchymal stem cells |
| spellingShingle | Raminta Vaiciuleviciute Ilona Uzieliene Paulius Bernotas Vitalij Novickij Aidas Alaburda Eiva Bernotiene Electrical Stimulation in Cartilage Tissue Engineering Bioengineering cartilage osteoarthritis chondrogenesis electrical stimulation mesenchymal stem cells |
| title | Electrical Stimulation in Cartilage Tissue Engineering |
| title_full | Electrical Stimulation in Cartilage Tissue Engineering |
| title_fullStr | Electrical Stimulation in Cartilage Tissue Engineering |
| title_full_unstemmed | Electrical Stimulation in Cartilage Tissue Engineering |
| title_short | Electrical Stimulation in Cartilage Tissue Engineering |
| title_sort | electrical stimulation in cartilage tissue engineering |
| topic | cartilage osteoarthritis chondrogenesis electrical stimulation mesenchymal stem cells |
| url | https://www.mdpi.com/2306-5354/10/4/454 |
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