Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae
Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016123005198 |
| _version_ | 1797388831161319424 |
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| author | Sam Edwards Henrik H. De Fine Licht |
| author_facet | Sam Edwards Henrik H. De Fine Licht |
| author_sort | Sam Edwards |
| collection | DOAJ |
| description | Insect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation. • E. muscae isolation and culturing in vitro. • Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica). • Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver. |
| first_indexed | 2024-03-08T22:46:31Z |
| format | Article |
| id | doaj.art-a6b9c395299d40509a96587f6d3813a1 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-03-08T22:46:31Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-a6b9c395299d40509a96587f6d3813a12023-12-17T06:38:28ZengElsevierMethodsX2215-01612024-06-0112102523Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscaeSam Edwards0Henrik H. De Fine Licht1Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C. 1871, Denmark; Living Systems Institute, Biosciencesv, University of Exeter, Exeter EX4 4QD, United Kingdom; Corresponding author.Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C. 1871, DenmarkInsect pathogenic fungi (IPF) and insects have ubiquitous interactions in nature. The extent of these interkingdom host-pathogen interactions are both complex and diverse. Some IPF, notably of the order Entomophthorales, manipulate their species-specific host before death. The fungus-induced altered insect behaviours are sequential and can accurately be repeatedly characterised temporally, making them a valuable model for understanding the molecular and chemical underpinnings of behaviour and host-pathogen co-evolutionary biology. Here, we present methods for the isolation and laboratory culturing of the emerging behaviourally manipulating model IPF Entomophthora muscae for experimentation. • E. muscae isolation and culturing in vitro. • Establishing and maintaining an E. muscae culture in vivo in houseflies (Musca domestica). • Controlled E. muscae infections for virulence experiments and quantification of conidia discharge per cadaver.http://www.sciencedirect.com/science/article/pii/S2215016123005198Isolation and culturing of Entomophthora muscae |
| spellingShingle | Sam Edwards Henrik H. De Fine Licht Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae MethodsX Isolation and culturing of Entomophthora muscae |
| title | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae |
| title_full | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae |
| title_fullStr | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae |
| title_full_unstemmed | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae |
| title_short | Rearing zombie flies: Laboratory culturing of the behaviourally manipulating fungal pathogen Entomophthora muscae |
| title_sort | rearing zombie flies laboratory culturing of the behaviourally manipulating fungal pathogen entomophthora muscae |
| topic | Isolation and culturing of Entomophthora muscae |
| url | http://www.sciencedirect.com/science/article/pii/S2215016123005198 |
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