Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components
Herein, we established the fingerprint of Ligusticum chuanxiong, and the effects of different 60Co-γ irradiation doses on the eight active components of Ligusticum chuanxiong were investigated. A stock solution of the eight standard reference substances in Ligusticum chuanxiong were analyzed using a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Science Press
2023-10-01
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| Series: | Fushe yanjiu yu fushe gongyi xuebao |
| Subjects: | |
| Online Access: | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2023-0007&lang=zh |
| _version_ | 1797524326097879040 |
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| author | QIU Yalu GAO Peng HE Jiang CHEN Qian WANG Yan |
| author_facet | QIU Yalu GAO Peng HE Jiang CHEN Qian WANG Yan |
| author_sort | QIU Yalu |
| collection | DOAJ |
| description | Herein, we established the fingerprint of Ligusticum chuanxiong, and the effects of different 60Co-γ irradiation doses on the eight active components of Ligusticum chuanxiong were investigated. A stock solution of the eight standard reference substances in Ligusticum chuanxiong were analyzed using a SHIMADZU Shim-pack GIST C18 chromatographic column (250 mm × 4.6 mm, 5 μm). Different doses (3 kGy, 6 kGy,10 kGy and 15 kGy) of 60Co-γ irradiation were used to treat Ligusticum chuanxiong. After these treatments, the fingerprint of Ligusticum chuanxiong was obtained via liquid chromatography. The similarity was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition), and the results indicated that the similarity of each component in Ligusticum chuanxiong was >0.988. After irradiation at <10 kGy, the contents of the eight active components in Ligusticum chuanxiong exhibited no evident differences, and the results indicated the stability and reliability of the method. Thus, this study provides a theoretical basis and support for the use of irradiation technology in the quality assurance of Ligusticum chuanxiong and the formulation of irradiation standards for Ligusticum chuanxiong. |
| first_indexed | 2024-03-10T08:55:46Z |
| format | Article |
| id | doaj.art-a6ba3d8dcfb9457f9766f614a516af0d |
| institution | Directory Open Access Journal |
| issn | 1000-3436 |
| language | zho |
| last_indexed | 2024-03-10T08:55:46Z |
| publishDate | 2023-10-01 |
| publisher | Science Press |
| record_format | Article |
| series | Fushe yanjiu yu fushe gongyi xuebao |
| spelling | doaj.art-a6ba3d8dcfb9457f9766f614a516af0d2023-11-22T07:09:08ZzhoScience PressFushe yanjiu yu fushe gongyi xuebao1000-34362023-10-0141505030305030310.11889/j.1000-3436.2023-00071000-3436（2023）05-0060-13Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active componentsQIU Yalu0GAO Peng1HE Jiang2CHEN Qian3WANG Yan4Sichuan Institute of Atomic Energy, Chengdu 610101, ChinaSichuan Institute of Atomic Energy, Chengdu 610101, ChinaSichuan Institute of Atomic Energy, Chengdu 610101, ChinaSichuan Institute of Atomic Energy, Chengdu 610101, ChinaSichuan Institute of Atomic Energy, Chengdu 610101, ChinaHerein, we established the fingerprint of Ligusticum chuanxiong, and the effects of different 60Co-γ irradiation doses on the eight active components of Ligusticum chuanxiong were investigated. A stock solution of the eight standard reference substances in Ligusticum chuanxiong were analyzed using a SHIMADZU Shim-pack GIST C18 chromatographic column (250 mm × 4.6 mm, 5 μm). Different doses (3 kGy, 6 kGy,10 kGy and 15 kGy) of 60Co-γ irradiation were used to treat Ligusticum chuanxiong. After these treatments, the fingerprint of Ligusticum chuanxiong was obtained via liquid chromatography. The similarity was evaluated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition), and the results indicated that the similarity of each component in Ligusticum chuanxiong was >0.988. After irradiation at <10 kGy, the contents of the eight active components in Ligusticum chuanxiong exhibited no evident differences, and the results indicated the stability and reliability of the method. Thus, this study provides a theoretical basis and support for the use of irradiation technology in the quality assurance of Ligusticum chuanxiong and the formulation of irradiation standards for Ligusticum chuanxiong.http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2023-0007&lang=zhligusticum chuanxiong60co-γ irradiationactive ingredientsfingerprints |
| spellingShingle | QIU Yalu GAO Peng HE Jiang CHEN Qian WANG Yan Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components Fushe yanjiu yu fushe gongyi xuebao ligusticum chuanxiong 60co-γ irradiation active ingredients fingerprints |
| title | Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components |
| title_full | Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components |
| title_fullStr | Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components |
| title_full_unstemmed | Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components |
| title_short | Establishing the fingerprints of Ligusticum chuanxiong and assessing the effects of 60Co-γ irradiation on its active components |
| title_sort | establishing the fingerprints of ligusticum chuanxiong and assessing the effects of 60co γ irradiation on its active components |
| topic | ligusticum chuanxiong 60co-γ irradiation active ingredients fingerprints |
| url | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2023-0007&lang=zh |
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