Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae
Background: In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared.Methods: Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran provinces, Iran, during 2008-2009. The snails were transported to the lab...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/upload_files/pdf/17756.pdf |
| _version_ | 1829487954188304384 |
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| author | AA Saboor Yaraghi A Farahnak MR Eshraghian |
| author_facet | AA Saboor Yaraghi A Farahnak MR Eshraghian |
| author_sort | AA Saboor Yaraghi |
| collection | DOAJ |
| description | Background: In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared.Methods: Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran provinces, Iran, during 2008-2009. The snails were transported to the lab at Tehran University of Medical Sciences and their cercarial sheddings were studied. Haemolmyphs of snails were extracted and cells were counted using haemocytometer and cell-surface carbohydrate were recognized by conjugated lectin (Lentil). Haemolymph protein concentrations were measured by Bradford protein assay method and soluble protein compositions were determined on sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE).Result: From the 550 examined Lymnaea snails for cercariae, 27 snails were infected with xiphidiocercariae. Mean of haemolymph cells (haemocyte) number were obtained 93480±2.43 (cells/ml) for none infected snails (25 snail) and 124560±2800 (cells/ml) for infected snails (25 snail). Mannose carbohydrate was recognized on haemocyte of none infected and infected snails. Mean of protein concentration of haemolymph plasma was obtained as 1354 ± 160 μg/ml (1.4 mg/ml) for none infected snails (25 snails) and 1802±138 μg/ml (1.8 mg/ml) for infected snail (25 snails). Comparing to none infected snails, the SDS-PAGE results of haemolymph plasma of infected snails, showed an extra protein band (70 kDa). The results showed a significant difference between the amounts and the kinds of proteins in haemolymph of infected and none infected snails.Conclusion: This information might be useful to understand of parasite detection, adhesion, engulfment and antigen agglutination by snail. |
| first_indexed | 2024-12-14T23:40:42Z |
| format | Article |
| id | doaj.art-a6bbf5faae4145dfadb51a3d188b7e4e |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-14T23:40:42Z |
| publishDate | 2011-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-a6bbf5faae4145dfadb51a3d188b7e4e2022-12-21T22:43:31ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2011-03-01618691Haemolymph Components of Infected & None Infected Lymnaea Snails with XiphidiocercariaeAA Saboor YaraghiA FarahnakMR EshraghianBackground: In this study the haemolymph components of infected and none infected Lymnaea gedrosiana with xiphidiocercaria larvae was compared.Methods: Five hundred Fifty Lymnaea snails were collected from Ilam and Mazandaran provinces, Iran, during 2008-2009. The snails were transported to the lab at Tehran University of Medical Sciences and their cercarial sheddings were studied. Haemolmyphs of snails were extracted and cells were counted using haemocytometer and cell-surface carbohydrate were recognized by conjugated lectin (Lentil). Haemolymph protein concentrations were measured by Bradford protein assay method and soluble protein compositions were determined on sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE).Result: From the 550 examined Lymnaea snails for cercariae, 27 snails were infected with xiphidiocercariae. Mean of haemolymph cells (haemocyte) number were obtained 93480±2.43 (cells/ml) for none infected snails (25 snail) and 124560±2800 (cells/ml) for infected snails (25 snail). Mannose carbohydrate was recognized on haemocyte of none infected and infected snails. Mean of protein concentration of haemolymph plasma was obtained as 1354 ± 160 μg/ml (1.4 mg/ml) for none infected snails (25 snails) and 1802±138 μg/ml (1.8 mg/ml) for infected snail (25 snails). Comparing to none infected snails, the SDS-PAGE results of haemolymph plasma of infected snails, showed an extra protein band (70 kDa). The results showed a significant difference between the amounts and the kinds of proteins in haemolymph of infected and none infected snails.Conclusion: This information might be useful to understand of parasite detection, adhesion, engulfment and antigen agglutination by snail.http://journals.tums.ac.ir/upload_files/pdf/17756.pdfCercariaLymnaeaHaemolymphXiphidiocercariae |
| spellingShingle | AA Saboor Yaraghi A Farahnak MR Eshraghian Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae Iranian Journal of Parasitology Cercaria Lymnaea Haemolymph Xiphidiocercariae |
| title | Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae |
| title_full | Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae |
| title_fullStr | Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae |
| title_full_unstemmed | Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae |
| title_short | Haemolymph Components of Infected & None Infected Lymnaea Snails with Xiphidiocercariae |
| title_sort | haemolymph components of infected none infected lymnaea snails with xiphidiocercariae |
| topic | Cercaria Lymnaea Haemolymph Xiphidiocercariae |
| url | http://journals.tums.ac.ir/upload_files/pdf/17756.pdf |
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