Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA
Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/21/6414 |
| _version_ | 1827678040110399488 |
|---|---|
| author | Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami |
| author_facet | Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami |
| author_sort | Hemalatha Murugaiah |
| collection | DOAJ |
| description | Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. <i>Streptomyces</i> sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against <i>P. aeruginosa</i> USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria <i>P. aeruginosa</i> and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site. |
| first_indexed | 2024-03-10T05:56:03Z |
| format | Article |
| id | doaj.art-a6bc46aaf4864a799edac219e7a571b8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:56:03Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a6bc46aaf4864a799edac219e7a571b82023-11-22T21:20:58ZengMDPI AGMolecules1420-30492021-10-012621641410.3390/molecules26216414Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSAHemalatha Murugaiah0Chow Lun Teh1Kai Chew Loh2Ahmad Ramli Mohamad Yahya3Nur Asshifa Md Noh4Noor Hana Hanif Abu Bakar5Daruliza Kernain6Rokiah Hashim7Yazmin Bustami8School of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Chemical Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Industrial Technology, Universiti Sains Malaysia, Gelugor 11700, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11700, MalaysiaHere, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. <i>Streptomyces</i> sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against <i>P. aeruginosa</i> USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria <i>P. aeruginosa</i> and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.https://www.mdpi.com/1420-3049/26/21/6414silver nanoparticles<i>Streptomyces</i> sp.biosynthesisantibacterialanticancercellulose nanocrystals |
| spellingShingle | Hemalatha Murugaiah Chow Lun Teh Kai Chew Loh Ahmad Ramli Mohamad Yahya Nur Asshifa Md Noh Noor Hana Hanif Abu Bakar Daruliza Kernain Rokiah Hashim Yazmin Bustami Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA Molecules silver nanoparticles <i>Streptomyces</i> sp. biosynthesis antibacterial anticancer cellulose nanocrystals |
| title | Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA |
| title_full | Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA |
| title_fullStr | Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA |
| title_full_unstemmed | Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA |
| title_short | Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using <i>Streptomyces</i> sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against <i>P. aeruginosa</i> USM-AR2 and MRSA |
| title_sort | study of antibacterial and anticancer properties of bioagnps synthesized using i streptomyces i sp pbd 311b and the application of bioagnp cnc alg as an antibacterial hydrogel film against i p aeruginosa i usm ar2 and mrsa |
| topic | silver nanoparticles <i>Streptomyces</i> sp. biosynthesis antibacterial anticancer cellulose nanocrystals |
| url | https://www.mdpi.com/1420-3049/26/21/6414 |
| work_keys_str_mv | AT hemalathamurugaiah studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT chowlunteh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT kaichewloh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT ahmadramlimohamadyahya studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT nurasshifamdnoh studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT noorhanahanifabubakar studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT darulizakernain studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT rokiahhashim studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa AT yazminbustami studyofantibacterialandanticancerpropertiesofbioagnpssynthesizedusingistreptomycesisppbd311bandtheapplicationofbioagnpcncalgasanantibacterialhydrogelfilmagainstipaeruginosaiusmar2andmrsa |