Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia
The aim was to study patterns of the nNOS, iNOS and eNOS expression in pancreatic islets in prepubescent male offsprings of female rats with experimental gestational diabetes mellitus. Materials and methods. The study was carried out in 10 prepubescent male offsprings of female rats with normal p...
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| Format: | Article |
| Language: | English |
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Zaporozhye State Medical University
2016-01-01
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| Series: | Zaporožskij Medicinskij Žurnal |
| Subjects: | |
| Online Access: | http://zmj.zsmu.edu.ua/article/view/64088/60671 |
| _version_ | 1811312504788221952 |
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| author | O. V. Gancheva Yu. M. Kolesnyk Yu. I. Vorodeeva |
| author_facet | O. V. Gancheva Yu. M. Kolesnyk Yu. I. Vorodeeva |
| author_sort | O. V. Gancheva |
| collection | DOAJ |
| description | The aim was to study patterns of the nNOS, iNOS and eNOS expression in pancreatic islets in prepubescent male offsprings of female rats with experimental gestational diabetes mellitus.
Materials and methods. The study was carried out in 10 prepubescent male offsprings of female rats with normal pregnancy and in 10 prepubescent male offsprings of female rats with experimental gestational diabetes mellitus. The expression of nNOS, iNOS and eNOS was evaluated in histological slices of pancreatic islets.
Results. It was found that the largest area of enzyme expression of eNOS was detected in control animals. On the other hand the immunoreactive material enzyme was maximal for iNOS. Chronic fetal hyperglycaemia affects the allocation of NOS isoforms in pancreatic islets. Higher immunoreactive material enzyme and nNOS contain, increase of iNOS area and the most significant increase of contain and area of fluorescence of eNOS compared with other expression indexes were detected. We believe the affection of glucose homeostasis in fetus during the last trimester of the pregnancy changes the relation of patterns of the expression of NOS isoforms, and it can lead to the formation of metabolic violation in adults. |
| first_indexed | 2024-04-13T10:38:23Z |
| format | Article |
| id | doaj.art-a6bebab513194f0199f222e0e96b2012 |
| institution | Directory Open Access Journal |
| issn | 2306-4145 2310-1210 |
| language | English |
| last_indexed | 2024-04-13T10:38:23Z |
| publishDate | 2016-01-01 |
| publisher | Zaporozhye State Medical University |
| record_format | Article |
| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj.art-a6bebab513194f0199f222e0e96b20122022-12-22T02:50:00ZengZaporozhye State Medical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102016-01-0113539http://dx.doi.org/10.14739/2310-1210.2016.1.64088Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemiaO. V. Gancheva Yu. M. KolesnykYu. I. VorodeevaThe aim was to study patterns of the nNOS, iNOS and eNOS expression in pancreatic islets in prepubescent male offsprings of female rats with experimental gestational diabetes mellitus. Materials and methods. The study was carried out in 10 prepubescent male offsprings of female rats with normal pregnancy and in 10 prepubescent male offsprings of female rats with experimental gestational diabetes mellitus. The expression of nNOS, iNOS and eNOS was evaluated in histological slices of pancreatic islets. Results. It was found that the largest area of enzyme expression of eNOS was detected in control animals. On the other hand the immunoreactive material enzyme was maximal for iNOS. Chronic fetal hyperglycaemia affects the allocation of NOS isoforms in pancreatic islets. Higher immunoreactive material enzyme and nNOS contain, increase of iNOS area and the most significant increase of contain and area of fluorescence of eNOS compared with other expression indexes were detected. We believe the affection of glucose homeostasis in fetus during the last trimester of the pregnancy changes the relation of patterns of the expression of NOS isoforms, and it can lead to the formation of metabolic violation in adults.http://zmj.zsmu.edu.ua/article/view/64088/60671NOS isoformsPancreatic IsletsGestational Diabetes MellitusExpression |
| spellingShingle | O. V. Gancheva Yu. M. Kolesnyk Yu. I. Vorodeeva Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia Zaporožskij Medicinskij Žurnal NOS isoforms Pancreatic Islets Gestational Diabetes Mellitus Expression |
| title | Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| title_full | Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| title_fullStr | Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| title_full_unstemmed | Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| title_short | Pathogenetical linkage of the Nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| title_sort | pathogenetical linkage of the nitric oxide synthase isoforms disbalance in pancreatic islets with chronical prenatal hyperglycemia |
| topic | NOS isoforms Pancreatic Islets Gestational Diabetes Mellitus Expression |
| url | http://zmj.zsmu.edu.ua/article/view/64088/60671 |
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