Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis
Incidence of mastitis may be associated with single nucleotide polymorphism (SNP). Cows (350) belonging to Sahiwal and Karan Fries were screened. Polymorphism of bovine lactoferrin gene promoter was determined by using created restriction site polymerase chain reaction-restriction fragment length p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-10-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/44255 |
| _version_ | 1797655907516022784 |
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| author | ALKA CHOPRA I D GUPTA ARCHANA VERMA V VOHRA |
| author_facet | ALKA CHOPRA I D GUPTA ARCHANA VERMA V VOHRA |
| author_sort | ALKA CHOPRA |
| collection | DOAJ |
| description |
Incidence of mastitis may be associated with single nucleotide polymorphism (SNP). Cows (350) belonging to Sahiwal and Karan Fries were screened. Polymorphism of bovine lactoferrin gene promoter was determined by using created restriction site polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results indicated that lactorferrin gene promoter is polymorphic and showed varied levels of polymorphism among Sahiwal and Karan Fries cattle. Three genotypes were identified, viz. EE, EF and FF in Sahiwal cattle and EE genotype was absent in Karan Fries cattle. Association of SNP identified with incidence of mastitis using chi square test, revealed nonsignificant association with mastitis incidence.
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| first_indexed | 2024-03-11T17:21:51Z |
| format | Article |
| id | doaj.art-a6bef5991aaa47d59b93596160b00b07 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-11T17:21:51Z |
| publishDate | 2014-10-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-a6bef5991aaa47d59b93596160b00b072023-10-19T11:14:13ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272014-10-01841010.56093/ijans.v84i10.44255Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitisALKA CHOPRA0I D GUPTA1ARCHANA VERMA2V VOHRA3National Dairy Research Institute, Karnal, Haryana 132001 IndiaNational Dairy Research Institute, Karnal, Haryana 132001 IndiaNational Dairy Research Institute, Karnal, Haryana 132001 IndiaNational Dairy Research Institute, Karnal, Haryana 132001 India Incidence of mastitis may be associated with single nucleotide polymorphism (SNP). Cows (350) belonging to Sahiwal and Karan Fries were screened. Polymorphism of bovine lactoferrin gene promoter was determined by using created restriction site polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results indicated that lactorferrin gene promoter is polymorphic and showed varied levels of polymorphism among Sahiwal and Karan Fries cattle. Three genotypes were identified, viz. EE, EF and FF in Sahiwal cattle and EE genotype was absent in Karan Fries cattle. Association of SNP identified with incidence of mastitis using chi square test, revealed nonsignificant association with mastitis incidence. https://epubs.icar.org.in/index.php/IJAnS/article/view/44255CattleLactoferrin genePolymerase chain reactionMastitisSahiwalKaran Fries |
| spellingShingle | ALKA CHOPRA I D GUPTA ARCHANA VERMA V VOHRA Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis Indian Journal of Animal Sciences Cattle Lactoferrin gene Polymerase chain reaction Mastitis Sahiwal Karan Fries |
| title | Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis |
| title_full | Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis |
| title_fullStr | Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis |
| title_full_unstemmed | Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis |
| title_short | Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis |
| title_sort | detection of allelic variants in lactoferrin gene promoter using created restriction site pcr rflp and its association with mastitis |
| topic | Cattle Lactoferrin gene Polymerase chain reaction Mastitis Sahiwal Karan Fries |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/44255 |
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