Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat
The use of antibiotics for therapeutic and especially non-therapeutic purposes in livestock farms promotes the development of antibiotic resistance in previously susceptible bacteria through selective pressure. In this work, we examined <i>E. coli</i> isolates using the standard Kirby-Ba...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/12/2217 |
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| author | Helen M. Sanchez Victoria A. Whitener Vanessa Thulsiraj Alicia Amundson Carolyn Collins Mckenzie Duran-Gonzalez Edwin Giragossian Allison Hornstra Sarah Kamel Andrea Maben Amelia Reynolds Elizabeth Roswell Benjamin Schmidt Lauren Sevigny Cindy Xiong Jennifer A. Jay |
| author_facet | Helen M. Sanchez Victoria A. Whitener Vanessa Thulsiraj Alicia Amundson Carolyn Collins Mckenzie Duran-Gonzalez Edwin Giragossian Allison Hornstra Sarah Kamel Andrea Maben Amelia Reynolds Elizabeth Roswell Benjamin Schmidt Lauren Sevigny Cindy Xiong Jennifer A. Jay |
| author_sort | Helen M. Sanchez |
| collection | DOAJ |
| description | The use of antibiotics for therapeutic and especially non-therapeutic purposes in livestock farms promotes the development of antibiotic resistance in previously susceptible bacteria through selective pressure. In this work, we examined <i>E. coli</i> isolates using the standard Kirby-Bauer disk diffusion susceptibility protocol and the CLSI standards. Companies selling retail chicken products in Los Angeles, California were grouped into three production groupings—<i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i>. <i>Humane Family Owned</i> is not a federally regulated category in the United States, but shows the reader that the chicken is incubated, hatched, raised, slaughtered, and packaged by one party, ensuring that the use of antibiotics in the entire production of the chicken is known and understood. We then examined the antibiotic resistance of the <i>E. coli</i> isolates (<i>n</i> = 325) by exposing them to seven common antibiotics, and resistance was seen to two of the antibiotics, ampicillin and erythromycin. As has been shown previously, it was found that for both ampicillin and erythromycin, there was no significant difference (<i>p</i> > 0.05) between <i>Conventional</i> and USDA (United States Department of Agriculture)-certified <i>No Antibiotics</i> chicken. Unique to this work, we additionally found that <i>Humane Family Owned</i> chicken had fewer (<i>p</i> ≤ 0.05) antibiotic-resistant <i>E. coli</i> isolates than both of the previous. Although not considered <i>directly</i> clinically relevant, we chose to test erythromycin because of its ecological significance to the environmental antibiotic resistome, which is not generally done. To our knowledge, <i>Humane Family Owned</i> consumer chicken has not previously been studied for its antibiotic resistance. This work contributes to a better understanding of a potential strategy of chicken production for the overall benefit of human health, giving evidentiary support to the One Health approach implemented by the World Health Organization. |
| first_indexed | 2024-03-10T14:33:13Z |
| format | Article |
| id | doaj.art-a6c0843e6bd443c1995b4727a2912e08 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:33:13Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a6c0843e6bd443c1995b4727a2912e082023-11-20T22:27:40ZengMDPI AGAnimals2076-26152020-11-011012221710.3390/ani10122217Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken MeatHelen M. Sanchez0Victoria A. Whitener1Vanessa Thulsiraj2Alicia Amundson3Carolyn Collins4Mckenzie Duran-Gonzalez5Edwin Giragossian6Allison Hornstra7Sarah Kamel8Andrea Maben9Amelia Reynolds10Elizabeth Roswell11Benjamin Schmidt12Lauren Sevigny13Cindy Xiong14Jennifer A. Jay15Department of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USAInstitute of the Environment and Sustainability, University of California at Los Angeles, Los Angeles, CA 90095, USADepartment of Civil and Environmental Engineering, University of California at Los Angeles, Los Angeles, CA 90095, USAThe use of antibiotics for therapeutic and especially non-therapeutic purposes in livestock farms promotes the development of antibiotic resistance in previously susceptible bacteria through selective pressure. In this work, we examined <i>E. coli</i> isolates using the standard Kirby-Bauer disk diffusion susceptibility protocol and the CLSI standards. Companies selling retail chicken products in Los Angeles, California were grouped into three production groupings—<i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i>. <i>Humane Family Owned</i> is not a federally regulated category in the United States, but shows the reader that the chicken is incubated, hatched, raised, slaughtered, and packaged by one party, ensuring that the use of antibiotics in the entire production of the chicken is known and understood. We then examined the antibiotic resistance of the <i>E. coli</i> isolates (<i>n</i> = 325) by exposing them to seven common antibiotics, and resistance was seen to two of the antibiotics, ampicillin and erythromycin. As has been shown previously, it was found that for both ampicillin and erythromycin, there was no significant difference (<i>p</i> > 0.05) between <i>Conventional</i> and USDA (United States Department of Agriculture)-certified <i>No Antibiotics</i> chicken. Unique to this work, we additionally found that <i>Humane Family Owned</i> chicken had fewer (<i>p</i> ≤ 0.05) antibiotic-resistant <i>E. coli</i> isolates than both of the previous. Although not considered <i>directly</i> clinically relevant, we chose to test erythromycin because of its ecological significance to the environmental antibiotic resistome, which is not generally done. To our knowledge, <i>Humane Family Owned</i> consumer chicken has not previously been studied for its antibiotic resistance. This work contributes to a better understanding of a potential strategy of chicken production for the overall benefit of human health, giving evidentiary support to the One Health approach implemented by the World Health Organization.https://www.mdpi.com/2076-2615/10/12/2217antibiotic resistanceantimicrobial resistancepoultrychickenampicillinerythromycin |
| spellingShingle | Helen M. Sanchez Victoria A. Whitener Vanessa Thulsiraj Alicia Amundson Carolyn Collins Mckenzie Duran-Gonzalez Edwin Giragossian Allison Hornstra Sarah Kamel Andrea Maben Amelia Reynolds Elizabeth Roswell Benjamin Schmidt Lauren Sevigny Cindy Xiong Jennifer A. Jay Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat Animals antibiotic resistance antimicrobial resistance poultry chicken ampicillin erythromycin |
| title | Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat |
| title_full | Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat |
| title_fullStr | Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat |
| title_full_unstemmed | Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat |
| title_short | Antibiotic Resistance of <i>Escherichia coli</i> Isolated from <i>Conventional</i>, <i>No Antibiotics</i>, and <i>Humane Family Owned</i> Retail Broiler Chicken Meat |
| title_sort | antibiotic resistance of i escherichia coli i isolated from i conventional i i no antibiotics i and i humane family owned i retail broiler chicken meat |
| topic | antibiotic resistance antimicrobial resistance poultry chicken ampicillin erythromycin |
| url | https://www.mdpi.com/2076-2615/10/12/2217 |
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