Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages

Abstract Objective Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for g...

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Bibliographic Details
Main Authors: Quynh Anh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata, Takeshige Otoi
Format: Article
Language:English
Published: BMC 2021-01-01
Series:BMC Research Notes
Subjects:
B4GALNT2
Electroporation
Microinjection
Pig
Xenotransplantation
Online Access:https://doi.org/10.1186/s13104-020-05412-8

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