Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5
Gram-negative bacterium Acinetobacter sp. Tol 5 exhibits high adhesiveness to various surfaces of general materials, from hydrophobic plastics to hydrophilic glass and metals, via AtaA, an Acinetobacter trimeric autotransporter adhesin Although the adhesion of Tol 5 is nonspecific, Tol 5 cells may h...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1342418/full |
| _version_ | 1827357903216967680 |
|---|---|
| author | Shogo Yoshimoto Satoshi Ishii Ayane Kawashiri Taishi Matsushita Dirk Linke Stephan Göttig Volkhard A. J. Kempf Madoka Takai Katsutoshi Hori |
| author_facet | Shogo Yoshimoto Satoshi Ishii Ayane Kawashiri Taishi Matsushita Dirk Linke Stephan Göttig Volkhard A. J. Kempf Madoka Takai Katsutoshi Hori |
| author_sort | Shogo Yoshimoto |
| collection | DOAJ |
| description | Gram-negative bacterium Acinetobacter sp. Tol 5 exhibits high adhesiveness to various surfaces of general materials, from hydrophobic plastics to hydrophilic glass and metals, via AtaA, an Acinetobacter trimeric autotransporter adhesin Although the adhesion of Tol 5 is nonspecific, Tol 5 cells may have prefer materials for adhesion. Here, we examined the adhesion of Tol 5 and other bacteria expressing different TAAs to various materials, including antiadhesive surfaces. The results highlighted the stickiness of Tol 5 through the action of AtaA, which enabled Tol 5 cells to adhere even to antiadhesive materials, including polytetrafluoroethylene with a low surface free energy, a hydrophilic polymer brush with steric hindrance, and mica with an ultrasmooth surface. Single-cell force spectroscopy as an atomic force microscopy technique revealed the strong cell adhesion force of Tol 5 to these antiadhesive materials. Nevertheless, Tol 5 cells showed a weak adhesion force toward a zwitterionic 2-methacryloyloxyethyl-phosphorylcholine (MPC) polymer-coated surface. Dynamic flow chamber experiments revealed that Tol 5 cells, once attached to the MPC polymer-coated surface, were exfoliated by weak shear stress. The underlying adhesive mechanism was presumed to involve exchangeable, weakly bound water molecules. Our results will contribute to the understanding and control of cell adhesion of Tol 5 for immobilized bioprocess applications and other TAA-expressing pathogenic bacteria of medical importance. |
| first_indexed | 2024-03-08T05:54:55Z |
| format | Article |
| id | doaj.art-a6c22f4e0cd141228702f9eb9cbe26b6 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T05:54:55Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a6c22f4e0cd141228702f9eb9cbe26b62024-02-05T04:36:02ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-02-011210.3389/fbioe.2024.13424181342418Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5Shogo Yoshimoto0Satoshi Ishii1Ayane Kawashiri2Taishi Matsushita3Dirk Linke4Stephan Göttig5Volkhard A. J. Kempf6Madoka Takai7Katsutoshi Hori8Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, JapanDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, JapanDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, JapanDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, JapanDepartment of Biosciences, University of Oslo, Oslo, NorwayInstitute for Medical Microbiology and Infection Control, University Hospital, Goethe University, Frankfurt, GermanyInstitute for Medical Microbiology and Infection Control, University Hospital, Goethe University, Frankfurt, GermanyDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, JapanDepartment of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, JapanGram-negative bacterium Acinetobacter sp. Tol 5 exhibits high adhesiveness to various surfaces of general materials, from hydrophobic plastics to hydrophilic glass and metals, via AtaA, an Acinetobacter trimeric autotransporter adhesin Although the adhesion of Tol 5 is nonspecific, Tol 5 cells may have prefer materials for adhesion. Here, we examined the adhesion of Tol 5 and other bacteria expressing different TAAs to various materials, including antiadhesive surfaces. The results highlighted the stickiness of Tol 5 through the action of AtaA, which enabled Tol 5 cells to adhere even to antiadhesive materials, including polytetrafluoroethylene with a low surface free energy, a hydrophilic polymer brush with steric hindrance, and mica with an ultrasmooth surface. Single-cell force spectroscopy as an atomic force microscopy technique revealed the strong cell adhesion force of Tol 5 to these antiadhesive materials. Nevertheless, Tol 5 cells showed a weak adhesion force toward a zwitterionic 2-methacryloyloxyethyl-phosphorylcholine (MPC) polymer-coated surface. Dynamic flow chamber experiments revealed that Tol 5 cells, once attached to the MPC polymer-coated surface, were exfoliated by weak shear stress. The underlying adhesive mechanism was presumed to involve exchangeable, weakly bound water molecules. Our results will contribute to the understanding and control of cell adhesion of Tol 5 for immobilized bioprocess applications and other TAA-expressing pathogenic bacteria of medical importance.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1342418/fullbacteriaadhesionantiadhesive materialsautotransportersAcinetobacter |
| spellingShingle | Shogo Yoshimoto Satoshi Ishii Ayane Kawashiri Taishi Matsushita Dirk Linke Stephan Göttig Volkhard A. J. Kempf Madoka Takai Katsutoshi Hori Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 Frontiers in Bioengineering and Biotechnology bacteria adhesion antiadhesive materials autotransporters Acinetobacter |
| title | Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 |
| title_full | Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 |
| title_fullStr | Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 |
| title_full_unstemmed | Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 |
| title_short | Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 |
| title_sort | adhesion preference of the sticky bacterium acinetobacter sp tol 5 |
| topic | bacteria adhesion antiadhesive materials autotransporters Acinetobacter |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1342418/full |
| work_keys_str_mv | AT shogoyoshimoto adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT satoshiishii adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT ayanekawashiri adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT taishimatsushita adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT dirklinke adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT stephangottig adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT volkhardajkempf adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT madokatakai adhesionpreferenceofthestickybacteriumacinetobactersptol5 AT katsutoshihori adhesionpreferenceofthestickybacteriumacinetobactersptol5 |