An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants
Paper mulberry (Broussonetia papyrifera) is a tree species that has many economic, ecological, and social uses. This study developed an efficient protocol for regenerating shoots from leaf explants using Murashige and Skoog (MS) medium supplemented with different concentrations of plant growth regul...
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-06-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2020-0034 |
| _version_ | 1819105925835784192 |
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| author | Cui Siming Ren Ying Hao Yahan Zhang Junjie Chen Zhouchao Zou Jintuo Zhou Wei Chen Xiaoyang |
| author_facet | Cui Siming Ren Ying Hao Yahan Zhang Junjie Chen Zhouchao Zou Jintuo Zhou Wei Chen Xiaoyang |
| author_sort | Cui Siming |
| collection | DOAJ |
| description | Paper mulberry (Broussonetia papyrifera) is a tree species that has many economic, ecological, and social uses. This study developed an efficient protocol for regenerating shoots from leaf explants using Murashige and Skoog (MS) medium supplemented with different concentrations of plant growth regulators (PGRs), which play vital roles in shoot regeneration. The best result, 86.67% induction frequency and 4.35 shoots per explant, was obtained in the MS medium containing 2.0 mg/L N6-benzyladenine (BA) and 0.05 mg/L indole-3-butyric acid. The effects of explant age, orientation, and genotype were also investigated. Explants from young leaves had a greater regeneration frequency than those from old leaves, and the results were better when the distal end of the leaf explant contacted the medium versus the proximal end. Approximately 70.96% of the shoots rooted well in the MS medium containing 0.4 mg/L α-naphthalene acetic acid (NAA). Although some genotypes achieved poorer results, the regeneration protocol is still applicable for mass multiplication and genetic transformation. |
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| format | Article |
| id | doaj.art-a6c3401e417c45f0a0f0c5503a8ccd6d |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-22T02:30:00Z |
| publishDate | 2020-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-a6c3401e417c45f0a0f0c5503a8ccd6d2022-12-21T18:41:54ZengDe GruyterOpen Life Sciences2391-54122020-06-0115131832510.1515/biol-2020-0034biol-2020-0034An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explantsCui Siming0Ren Ying1Hao Yahan2Zhang Junjie3Chen Zhouchao4Zou Jintuo5Zhou Wei6Chen Xiaoyang7State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (South China Agricultural University), Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaPaper mulberry (Broussonetia papyrifera) is a tree species that has many economic, ecological, and social uses. This study developed an efficient protocol for regenerating shoots from leaf explants using Murashige and Skoog (MS) medium supplemented with different concentrations of plant growth regulators (PGRs), which play vital roles in shoot regeneration. The best result, 86.67% induction frequency and 4.35 shoots per explant, was obtained in the MS medium containing 2.0 mg/L N6-benzyladenine (BA) and 0.05 mg/L indole-3-butyric acid. The effects of explant age, orientation, and genotype were also investigated. Explants from young leaves had a greater regeneration frequency than those from old leaves, and the results were better when the distal end of the leaf explant contacted the medium versus the proximal end. Approximately 70.96% of the shoots rooted well in the MS medium containing 0.4 mg/L α-naphthalene acetic acid (NAA). Although some genotypes achieved poorer results, the regeneration protocol is still applicable for mass multiplication and genetic transformation.https://doi.org/10.1515/biol-2020-0034paper mulberryleaf explantplant regenerationexplant ageinoculation orientation |
| spellingShingle | Cui Siming Ren Ying Hao Yahan Zhang Junjie Chen Zhouchao Zou Jintuo Zhou Wei Chen Xiaoyang An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants Open Life Sciences paper mulberry leaf explant plant regeneration explant age inoculation orientation |
| title | An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants |
| title_full | An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants |
| title_fullStr | An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants |
| title_full_unstemmed | An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants |
| title_short | An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants |
| title_sort | efficient protocol for regenerating shoots from paper mulberry broussonetia papyrifera leaf explants |
| topic | paper mulberry leaf explant plant regeneration explant age inoculation orientation |
| url | https://doi.org/10.1515/biol-2020-0034 |
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