Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i>
<i>Suaeda aralocaspica</i> is an annual halophyte in the Amaranthaceae in the saline deserts of central Asia. This plant has succulent leaves and grape-like fruits and is a potential horticultural plant. To obtain the efficient sterilization method and optimal culture conditions, two typ...
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MDPI AG
2022-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/4/289 |
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| author | Yu Si Yakupjan Haxim Lei Wang |
| author_facet | Yu Si Yakupjan Haxim Lei Wang |
| author_sort | Yu Si |
| collection | DOAJ |
| description | <i>Suaeda aralocaspica</i> is an annual halophyte in the Amaranthaceae in the saline deserts of central Asia. This plant has succulent leaves and grape-like fruits and is a potential horticultural plant. To obtain the efficient sterilization method and optimal culture conditions, two types of seeds produced from a single plant of <i>S. aralocaspica</i> were treated with 75% ethanol for different time durations first, and then sodium hypochlorite (NaClO) or mercury chloride (HgCl<sub>2</sub>), with five different timing treatments were used for second seed surface sterilization. Sterilized seeds were germinated on a Murashige and Skoog (MS) medium at different potential hydrogenation (pH) levels, to examine germination and seedling performance. The results showed that the highest germination percentage of brown seeds was 100% and that of black seeds was 17%. Thus, brown seeds were more suitable for further culture experiments than black seeds. For brown seeds, the sterilization effect of NaClO was better than that of HgCl<sub>2,</sub> based on the results of seed germination, contamination, and seedling survival. Rinsing with 75% ethanol for 60 s, sterilizing with NaClO for 8 min, and cultivating at pH 8.0 MS for 7 days was the best of all sterilization procedures and cultivation methods tested, which has been successfully applied to <i>S. aralocaspica</i> in vitro culture. The optimized protocol described here can be used as the reference for the <i>Suaeda</i> genus. |
| first_indexed | 2024-03-09T10:35:21Z |
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| language | English |
| last_indexed | 2024-03-09T10:35:21Z |
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| spelling | doaj.art-a6c51cfa599b45569d88fc62e548333d2023-12-01T21:00:14ZengMDPI AGHorticulturae2311-75242022-03-018428910.3390/horticulturae8040289Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i>Yu Si0Yakupjan Haxim1Lei Wang2State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Geography and Ecology, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Geography and Ecology, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Geography and Ecology, Chinese Academy of Sciences, Urumqi 830011, China<i>Suaeda aralocaspica</i> is an annual halophyte in the Amaranthaceae in the saline deserts of central Asia. This plant has succulent leaves and grape-like fruits and is a potential horticultural plant. To obtain the efficient sterilization method and optimal culture conditions, two types of seeds produced from a single plant of <i>S. aralocaspica</i> were treated with 75% ethanol for different time durations first, and then sodium hypochlorite (NaClO) or mercury chloride (HgCl<sub>2</sub>), with five different timing treatments were used for second seed surface sterilization. Sterilized seeds were germinated on a Murashige and Skoog (MS) medium at different potential hydrogenation (pH) levels, to examine germination and seedling performance. The results showed that the highest germination percentage of brown seeds was 100% and that of black seeds was 17%. Thus, brown seeds were more suitable for further culture experiments than black seeds. For brown seeds, the sterilization effect of NaClO was better than that of HgCl<sub>2,</sub> based on the results of seed germination, contamination, and seedling survival. Rinsing with 75% ethanol for 60 s, sterilizing with NaClO for 8 min, and cultivating at pH 8.0 MS for 7 days was the best of all sterilization procedures and cultivation methods tested, which has been successfully applied to <i>S. aralocaspica</i> in vitro culture. The optimized protocol described here can be used as the reference for the <i>Suaeda</i> genus.https://www.mdpi.com/2311-7524/8/4/289sodium hypochloritemercury chloridesterilization timeseed germination |
| spellingShingle | Yu Si Yakupjan Haxim Lei Wang Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> Horticulturae sodium hypochlorite mercury chloride sterilization time seed germination |
| title | Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> |
| title_full | Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> |
| title_fullStr | Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> |
| title_full_unstemmed | Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> |
| title_short | Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte <i>Suaeda aralocaspica</i> |
| title_sort | optimum sterilization method for in vitro cultivation of dimorphic seeds of the succulent halophyte i suaeda aralocaspica i |
| topic | sodium hypochlorite mercury chloride sterilization time seed germination |
| url | https://www.mdpi.com/2311-7524/8/4/289 |
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