Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice
Coreopsis tinctoria (CT) improves energy metabolism. However, the role of CT in alleviating obesity-induced hyperglycemia by targeting the liver remains unknown. Therefore, this article aims to explore the mechanism by which CT improves energy metabolism and resists hyperglycemia. The water and etha...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024034807 |
| _version_ | 1797224283461648384 |
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| author | Bingxin Huangfu Minglan Yang Jia Xu Ruxin Gao Yanzhou Hu Yijia Zhao Kunlun Huang Xiaoyun He |
| author_facet | Bingxin Huangfu Minglan Yang Jia Xu Ruxin Gao Yanzhou Hu Yijia Zhao Kunlun Huang Xiaoyun He |
| author_sort | Bingxin Huangfu |
| collection | DOAJ |
| description | Coreopsis tinctoria (CT) improves energy metabolism. However, the role of CT in alleviating obesity-induced hyperglycemia by targeting the liver remains unknown. Therefore, this article aims to explore the mechanism by which CT improves energy metabolism and resists hyperglycemia. The water and ethanol extracts of CT were administered to high-fat diet-induced (HFD) obese C57BL/6J mice at a dose of 4 g/kg.bw (low-dose water extract, WL; low-dose ethanol extract, EL) or 10 g/kg.bw (high-dose water extract, WH; high-dose ethanol extract, EH). Mice that consumed a maintenance diet (LFD) were included as blank controls. Network pharmacology, liquid chromatography-mass spectrometry (LC-MS), L02 cell cultivation, and liver transcriptomics were used to examine the mechanism and functional components of CT against obesity-induced hyperglycemia. The results indicated that WL significantly (p < 0.05) alleviated glucose intolerance and insulin resistance in obesity-induced hyperglycemia. Kaempferol is the main active compound of CT, which demonstrated significant (p < 0.05) anti-hyperglycemic effects in obese mice and L02 cells. Finally, kaempferol significantly (p < 0.05; fold change >1.2) shifted the genes involved in carbon metabolism, glycolysis/gluconeogenesis, and the mitogen-activated protein kinase (MAPK) pathways toward the trend of LFD, indicating that it exerts an anti-hyperglycemic effect through these molecular mechanisms. Overall, oral intake of CT lowers blood glucose and improves insulin sensitivity in mice with obesity-induced hyperglycemia. Kaempferol is the primary functional component of CT. |
| first_indexed | 2024-04-24T13:50:39Z |
| format | Article |
| id | doaj.art-a6cc34b0836148bfac08d3d49674a362 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:39Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-a6cc34b0836148bfac08d3d49674a3622024-04-04T05:04:52ZengElsevierHeliyon2405-84402024-03-01106e27449Coreopsis tinctoria improves energy metabolism in obese hyperglycemic miceBingxin Huangfu0Minglan Yang1Jia Xu2Ruxin Gao3Yanzhou Hu4Yijia Zhao5Kunlun Huang6Xiaoyun He7Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Department of Clinical Nutrition, West China Hospital of Sichuan University, Chengdu, 610041, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, ChinaKey Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the PR China, Beijing, 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the PR China, Beijing, 100083, ChinaKey Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the PR China, Beijing, 100083, China; Corresponding author. Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.Coreopsis tinctoria (CT) improves energy metabolism. However, the role of CT in alleviating obesity-induced hyperglycemia by targeting the liver remains unknown. Therefore, this article aims to explore the mechanism by which CT improves energy metabolism and resists hyperglycemia. The water and ethanol extracts of CT were administered to high-fat diet-induced (HFD) obese C57BL/6J mice at a dose of 4 g/kg.bw (low-dose water extract, WL; low-dose ethanol extract, EL) or 10 g/kg.bw (high-dose water extract, WH; high-dose ethanol extract, EH). Mice that consumed a maintenance diet (LFD) were included as blank controls. Network pharmacology, liquid chromatography-mass spectrometry (LC-MS), L02 cell cultivation, and liver transcriptomics were used to examine the mechanism and functional components of CT against obesity-induced hyperglycemia. The results indicated that WL significantly (p < 0.05) alleviated glucose intolerance and insulin resistance in obesity-induced hyperglycemia. Kaempferol is the main active compound of CT, which demonstrated significant (p < 0.05) anti-hyperglycemic effects in obese mice and L02 cells. Finally, kaempferol significantly (p < 0.05; fold change >1.2) shifted the genes involved in carbon metabolism, glycolysis/gluconeogenesis, and the mitogen-activated protein kinase (MAPK) pathways toward the trend of LFD, indicating that it exerts an anti-hyperglycemic effect through these molecular mechanisms. Overall, oral intake of CT lowers blood glucose and improves insulin sensitivity in mice with obesity-induced hyperglycemia. Kaempferol is the primary functional component of CT.http://www.sciencedirect.com/science/article/pii/S2405844024034807Coreopsis tinctoriaObesityHyperglycemiaLiverKaempferol |
| spellingShingle | Bingxin Huangfu Minglan Yang Jia Xu Ruxin Gao Yanzhou Hu Yijia Zhao Kunlun Huang Xiaoyun He Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice Heliyon Coreopsis tinctoria Obesity Hyperglycemia Liver Kaempferol |
| title | Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| title_full | Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| title_fullStr | Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| title_full_unstemmed | Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| title_short | Coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| title_sort | coreopsis tinctoria improves energy metabolism in obese hyperglycemic mice |
| topic | Coreopsis tinctoria Obesity Hyperglycemia Liver Kaempferol |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024034807 |
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