Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng
Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-01-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845316302743 |
| _version_ | 1818062938229964800 |
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| author | Yo Han Hong Donghyun Kim Gibaeg Nam Sulgi Yoo Sang Yun Han Seong-Gu Jeong Eunji Kim Deok Jeong Keejung Yoon Sunggyu Kim Junseong Park Jae Youl Cho |
| author_facet | Yo Han Hong Donghyun Kim Gibaeg Nam Sulgi Yoo Sang Yun Han Seong-Gu Jeong Eunji Kim Deok Jeong Keejung Yoon Sunggyu Kim Junseong Park Jae Youl Cho |
| author_sort | Yo Han Hong |
| collection | DOAJ |
| description | Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events.
Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry. We also evaluated the effects of BIOGF1K on the activator protein-1 signaling pathway, which plays an important role in photoaging, by immunoblot analysis and luciferase reporter gene assays.
Results: Treatment of UVB-irradiated NIH3T3 fibroblasts with BIOGF1K prevented UVB-induced cell death, inhibited apoptosis, suppressed morphological changes, reduced melanin secretion, restored the levels of type I procollagen and sirtuin 1, and prevented mRNA upregulation of MMP-1, MMP-2, and cyclo-oxygenase-2; these effects all occurred in a dose-dependent manner. In addition, BIOGF1K markedly reduced activator-protein-1-mediated luciferase activity and decreased the activity of mitogen-activated protein kinases (extracellular response kinase, p38, and C-Jun N-terminal kinase).
Conclusion: Our results strongly suggest that BIOGF1K has anti-photoaging activity and that BIOGF1K could be used in anti-aging cosmeceutical preparations. |
| first_indexed | 2024-12-10T14:12:09Z |
| format | Article |
| id | doaj.art-a6cd75a8f00d4ad695854497900321b7 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-10T14:12:09Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-a6cd75a8f00d4ad695854497900321b72022-12-22T01:45:27ZengElsevierJournal of Ginseng Research1226-84532018-01-01421818910.1016/j.jgr.2017.01.002Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginsengYo Han Hong0Donghyun Kim1Gibaeg Nam2Sulgi Yoo3Sang Yun Han4Seong-Gu Jeong5Eunji Kim6Deok Jeong7Keejung Yoon8Sunggyu Kim9Junseong Park10Jae Youl Cho11Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaAmorepacific Research and Development Unit, Yongin, Republic of KoreaAmorepacific Research and Development Unit, Yongin, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaResearch and Business Foundation, Sungkyunkwan University, Suwon, Republic of KoreaAmorepacific Research and Development Unit, Yongin, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of KoreaBackground: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events. Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry. We also evaluated the effects of BIOGF1K on the activator protein-1 signaling pathway, which plays an important role in photoaging, by immunoblot analysis and luciferase reporter gene assays. Results: Treatment of UVB-irradiated NIH3T3 fibroblasts with BIOGF1K prevented UVB-induced cell death, inhibited apoptosis, suppressed morphological changes, reduced melanin secretion, restored the levels of type I procollagen and sirtuin 1, and prevented mRNA upregulation of MMP-1, MMP-2, and cyclo-oxygenase-2; these effects all occurred in a dose-dependent manner. In addition, BIOGF1K markedly reduced activator-protein-1-mediated luciferase activity and decreased the activity of mitogen-activated protein kinases (extracellular response kinase, p38, and C-Jun N-terminal kinase). Conclusion: Our results strongly suggest that BIOGF1K has anti-photoaging activity and that BIOGF1K could be used in anti-aging cosmeceutical preparations.http://www.sciencedirect.com/science/article/pii/S1226845316302743anti-agingBIOGF1KPanax ginsengphotoagingUVB irradiation |
| spellingShingle | Yo Han Hong Donghyun Kim Gibaeg Nam Sulgi Yoo Sang Yun Han Seong-Gu Jeong Eunji Kim Deok Jeong Keejung Yoon Sunggyu Kim Junseong Park Jae Youl Cho Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng Journal of Ginseng Research anti-aging BIOGF1K Panax ginseng photoaging UVB irradiation |
| title | Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng |
| title_full | Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng |
| title_fullStr | Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng |
| title_full_unstemmed | Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng |
| title_short | Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng |
| title_sort | photoaging protective effects of biogf1k a compound k rich fraction prepared from panax ginseng |
| topic | anti-aging BIOGF1K Panax ginseng photoaging UVB irradiation |
| url | http://www.sciencedirect.com/science/article/pii/S1226845316302743 |
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