Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation
Metal-induced oxidative stress in contaminated soils affects plant growth. In the present study, we evaluated the role of seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation. A series of pot experiments were conducted under variable Zn (500, 1,000, and 1,500 mg kg–1) and C...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.995830/full |
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| author | Vijay K. Sharma Shobhika Parmar Wenting Tang Haiyan Hu James F. White Haiyan Li |
| author_facet | Vijay K. Sharma Shobhika Parmar Wenting Tang Haiyan Hu James F. White Haiyan Li |
| author_sort | Vijay K. Sharma |
| collection | DOAJ |
| description | Metal-induced oxidative stress in contaminated soils affects plant growth. In the present study, we evaluated the role of seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation. A series of pot experiments were conducted under variable Zn (500, 1,000, and 1,500 mg kg–1) and Cd (5, 15, 30, and 60 mg kg–1). The results demonstrated that FXZ2-inoculation significantly enhanced the growth of D. ambrosioides and improved its chlorophyll and GSH content. In the rhizosphere, FXZ2 inoculation changed the chemical speciation of Zn/Cd and thus affected their uptake and accumulation in host plants. The exchangeable and carbonate-bound fractions (F1 + F2) of Zn decreased in the rhizosphere of FXZ2-inoculated plants (E+) as compared to non-inoculated plants (E-) under Zn stress (500 and 1,000 mg kg–1), correspondingly, Zn in the shoots of E+ decreased (p < 0.05). However, at Cd stress (30 and 60 mg kg–1), the F1 + F2 fractions of Cd in E+ rhizospheric soils increased; subsequently, Cd in the shoots of E+ increased (p < 0.05). FXZ2 could exogenously secrete phytohormones IAA, GA, and JA. The study suggests that seed endophyte FXZ2 can increase Zn/Cd tolerance of host plant by altering Zn/Cd speciation in rhizospheric soils, as well as exogenous production of phytohormones to promote growth, lowering oxidative damage while enhancing antioxidant properties. For Zn/Cd accumulation, it has opposite effects: Zn uptake in E+ plants was significantly (p < 0.05) decreased, while Cd accumulation in E+ plants was significantly (p < 0.05) increased. Thus, FXZ2 has excellent application prospects in Cd phytoextraction and decreasing Zn toxicity in agriculturally important crops. |
| first_indexed | 2024-04-11T20:02:46Z |
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| id | doaj.art-a6cd89f70fcf4647b340f04a0904d66a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T20:02:46Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a6cd89f70fcf4647b340f04a0904d66a2022-12-22T04:05:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.995830995830Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulationVijay K. Sharma0Shobhika Parmar1Wenting Tang2Haiyan Hu3James F. White4Haiyan Li5Medical School, Kunming University of Science and Technology, Kunming, ChinaMedical School, Kunming University of Science and Technology, Kunming, ChinaMedical School, Kunming University of Science and Technology, Kunming, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, ChinaDepartment of Plant Biology, Rutgers University, New Brunswick, NJ, United StatesMedical School, Kunming University of Science and Technology, Kunming, ChinaMetal-induced oxidative stress in contaminated soils affects plant growth. In the present study, we evaluated the role of seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation. A series of pot experiments were conducted under variable Zn (500, 1,000, and 1,500 mg kg–1) and Cd (5, 15, 30, and 60 mg kg–1). The results demonstrated that FXZ2-inoculation significantly enhanced the growth of D. ambrosioides and improved its chlorophyll and GSH content. In the rhizosphere, FXZ2 inoculation changed the chemical speciation of Zn/Cd and thus affected their uptake and accumulation in host plants. The exchangeable and carbonate-bound fractions (F1 + F2) of Zn decreased in the rhizosphere of FXZ2-inoculated plants (E+) as compared to non-inoculated plants (E-) under Zn stress (500 and 1,000 mg kg–1), correspondingly, Zn in the shoots of E+ decreased (p < 0.05). However, at Cd stress (30 and 60 mg kg–1), the F1 + F2 fractions of Cd in E+ rhizospheric soils increased; subsequently, Cd in the shoots of E+ increased (p < 0.05). FXZ2 could exogenously secrete phytohormones IAA, GA, and JA. The study suggests that seed endophyte FXZ2 can increase Zn/Cd tolerance of host plant by altering Zn/Cd speciation in rhizospheric soils, as well as exogenous production of phytohormones to promote growth, lowering oxidative damage while enhancing antioxidant properties. For Zn/Cd accumulation, it has opposite effects: Zn uptake in E+ plants was significantly (p < 0.05) decreased, while Cd accumulation in E+ plants was significantly (p < 0.05) increased. Thus, FXZ2 has excellent application prospects in Cd phytoextraction and decreasing Zn toxicity in agriculturally important crops.https://www.frontiersin.org/articles/10.3389/fmicb.2022.995830/fullseed endophytesDysphania ambrosioidesmetal stressphytoremediationmechanismchemical speciation |
| spellingShingle | Vijay K. Sharma Shobhika Parmar Wenting Tang Haiyan Hu James F. White Haiyan Li Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation Frontiers in Microbiology seed endophytes Dysphania ambrosioides metal stress phytoremediation mechanism chemical speciation |
| title | Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation |
| title_full | Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation |
| title_fullStr | Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation |
| title_full_unstemmed | Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation |
| title_short | Effects of fungal seed endophyte FXZ2 on Dysphania ambrosioides Zn/Cd tolerance and accumulation |
| title_sort | effects of fungal seed endophyte fxz2 on dysphania ambrosioides zn cd tolerance and accumulation |
| topic | seed endophytes Dysphania ambrosioides metal stress phytoremediation mechanism chemical speciation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.995830/full |
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