Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays
Obesity is a chronic disease affecting both adults and children worldwide. One major cause of obesity is high-calorie intake due to overconsumption of foods rich in fat and carbohydrates. Hence, obesity can be controlled by controlling the diet and by other lifestyle changes that increase energy exp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Obesities |
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| Online Access: | https://www.mdpi.com/2673-4168/3/1/3 |
| _version_ | 1797609656508481536 |
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| author | Subhani Bandara Shelby Devereaux Aruna Weerasooriya |
| author_facet | Subhani Bandara Shelby Devereaux Aruna Weerasooriya |
| author_sort | Subhani Bandara |
| collection | DOAJ |
| description | Obesity is a chronic disease affecting both adults and children worldwide. One major cause of obesity is high-calorie intake due to overconsumption of foods rich in fat and carbohydrates. Hence, obesity can be controlled by controlling the diet and by other lifestyle changes that increase energy expenditure. However, this is not always possible for individuals who are already overweight and suffering from other diseases. Therefore, certain drugs have been developed to assist with weight reduction. One major avenue for drug development involves the inhibition of enzymes that break down fat and carbohydrates from the diet. This can reduce the bioavailability and absorption of dietary lipids and carbohydrates, allowing for the management of obesity. Although there are synthetic drugs available on the market to inhibit these enzymes, plant-based natural drugs may provide a better alternative to treat obesity due to fewer side effects and a lower cost. In this review, different methods that can be used to screen medicinal plant extracts for inhibitors of those digestive enzymes and certain limitations of those methods are discussed. Currently, there is limited research on the effects of varying conditions on enzyme assays, and this is an area that can be addressed in future research. |
| first_indexed | 2024-03-11T06:04:26Z |
| format | Article |
| id | doaj.art-a6ce09645caa491785d4f3c4535a3359 |
| institution | Directory Open Access Journal |
| issn | 2673-4168 |
| language | English |
| last_indexed | 2024-03-11T06:04:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Obesities |
| spelling | doaj.art-a6ce09645caa491785d4f3c4535a33592023-11-17T13:07:21ZengMDPI AGObesities2673-41682023-01-0131133510.3390/obesities3010003Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme AssaysSubhani Bandara0Shelby Devereaux1Aruna Weerasooriya2Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USACooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USACooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, USAObesity is a chronic disease affecting both adults and children worldwide. One major cause of obesity is high-calorie intake due to overconsumption of foods rich in fat and carbohydrates. Hence, obesity can be controlled by controlling the diet and by other lifestyle changes that increase energy expenditure. However, this is not always possible for individuals who are already overweight and suffering from other diseases. Therefore, certain drugs have been developed to assist with weight reduction. One major avenue for drug development involves the inhibition of enzymes that break down fat and carbohydrates from the diet. This can reduce the bioavailability and absorption of dietary lipids and carbohydrates, allowing for the management of obesity. Although there are synthetic drugs available on the market to inhibit these enzymes, plant-based natural drugs may provide a better alternative to treat obesity due to fewer side effects and a lower cost. In this review, different methods that can be used to screen medicinal plant extracts for inhibitors of those digestive enzymes and certain limitations of those methods are discussed. Currently, there is limited research on the effects of varying conditions on enzyme assays, and this is an area that can be addressed in future research.https://www.mdpi.com/2673-4168/3/1/3obesityenzyme assay methodmedicinal plantsenzyme inhibitorspancreatic lipasecholesterol esterase |
| spellingShingle | Subhani Bandara Shelby Devereaux Aruna Weerasooriya Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays Obesities obesity enzyme assay method medicinal plants enzyme inhibitors pancreatic lipase cholesterol esterase |
| title | Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays |
| title_full | Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays |
| title_fullStr | Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays |
| title_full_unstemmed | Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays |
| title_short | Methods to Evaluate the Antiobesity Effects of Medicinal Plants Using Enzyme Assays |
| title_sort | methods to evaluate the antiobesity effects of medicinal plants using enzyme assays |
| topic | obesity enzyme assay method medicinal plants enzyme inhibitors pancreatic lipase cholesterol esterase |
| url | https://www.mdpi.com/2673-4168/3/1/3 |
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