Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas
The microbiota of fish intestine is the most complex of all organs that interact closely with the immune system. This can be used as biological control agents that can control the disease caused by pathogenic bacteria. Thirty isolates were identified based on 16s rRNA gene sequencing. These bacteri...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2018-06-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/intestinal-bacteria-of-common-carp-cyprinus-carpio-l-as-a-biological-control-agent-for-aeromonas/ |
| _version_ | 1828952333182042112 |
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| author | Yuniar Mulyani I. Nyoman P. Aryantha Sony Suhandono Adi Pancoro |
| author_facet | Yuniar Mulyani I. Nyoman P. Aryantha Sony Suhandono Adi Pancoro |
| author_sort | Yuniar Mulyani |
| collection | DOAJ |
| description | The microbiota of fish intestine is the most complex of all organs that interact closely with the immune system. This can be used as biological control agents that can control the disease caused by pathogenic bacteria. Thirty isolates were identified based on 16s rRNA gene sequencing. These bacteria were then tested for their pathogenicity and antagonistic activity. The bioassay was conducted by administering selected bacteria into the fish and inoculating with Aeromonas. Total 30 bacterial isolates were successfully isolated from the carp intestine. Morphology and DNA marker analysis shows wide diversity of bacterial consortium within the fish intestine. These bacterial community are dominated by Proteobacteria and Firmicutes. The pathogenicity test showed that 20 isolates were non-pathogenic at a density of 106 cfu/mL, while the rest were pathogenic to the fish. The antagonistic test showed that some isolates strongly or mildly inhibit Aeromonas and Vibrio and the rest weakly or do not inhibit the assayed bacteria. Two isolates (CgM8=Bacillus sp. and CgM37=Bacillus subtilis) are significantly better than control to protect the fish from Aeromonas infection. Two species of commensal bacteria originated from fish intestine are potential to be used as biological control agents against Aeromonas for common carp. |
| first_indexed | 2024-12-14T06:51:49Z |
| format | Article |
| id | doaj.art-a6ceb4c57e164afba01eaacc281fded4 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-14T06:51:49Z |
| publishDate | 2018-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-a6ceb4c57e164afba01eaacc281fded42022-12-21T23:12:53ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-06-0112260161010.22207/JPAM.12.2.18Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for AeromonasYuniar Mulyani0I. Nyoman P. Aryantha1Sony Suhandono2Adi Pancoro31School of Life Sciences and Technology, Ganesha 10 Bandung, Indonesia. 3Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Bandung Jl. Raya Bandung-Sumedang KM.21, Jatinangor 45363, Indonesia.1School of Life Sciences and Technology, 2Biosciences and Biotechnology Research Group Institut Teknologi Bandung, Jl. Ganesha 10 Bandung, Indonesia.1School of Life Sciences and Technology, Jl. Ganesha 10 Bandung, Indonesia.1School of Life Sciences and Technology, Jl. Ganesha 10 Bandung, Indonesia.The microbiota of fish intestine is the most complex of all organs that interact closely with the immune system. This can be used as biological control agents that can control the disease caused by pathogenic bacteria. Thirty isolates were identified based on 16s rRNA gene sequencing. These bacteria were then tested for their pathogenicity and antagonistic activity. The bioassay was conducted by administering selected bacteria into the fish and inoculating with Aeromonas. Total 30 bacterial isolates were successfully isolated from the carp intestine. Morphology and DNA marker analysis shows wide diversity of bacterial consortium within the fish intestine. These bacterial community are dominated by Proteobacteria and Firmicutes. The pathogenicity test showed that 20 isolates were non-pathogenic at a density of 106 cfu/mL, while the rest were pathogenic to the fish. The antagonistic test showed that some isolates strongly or mildly inhibit Aeromonas and Vibrio and the rest weakly or do not inhibit the assayed bacteria. Two isolates (CgM8=Bacillus sp. and CgM37=Bacillus subtilis) are significantly better than control to protect the fish from Aeromonas infection. Two species of commensal bacteria originated from fish intestine are potential to be used as biological control agents against Aeromonas for common carp.https://microbiologyjournal.org/intestinal-bacteria-of-common-carp-cyprinus-carpio-l-as-a-biological-control-agent-for-aeromonas/aeromonas; biological control; cyprinus carpio; intestinal bacteria |
| spellingShingle | Yuniar Mulyani I. Nyoman P. Aryantha Sony Suhandono Adi Pancoro Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas Journal of Pure and Applied Microbiology aeromonas; biological control; cyprinus carpio; intestinal bacteria |
| title | Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas |
| title_full | Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas |
| title_fullStr | Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas |
| title_full_unstemmed | Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas |
| title_short | Intestinal Bacteria of Common Carp (Cyprinus carpio L.) as a Biological Control Agent for Aeromonas |
| title_sort | intestinal bacteria of common carp cyprinus carpio l as a biological control agent for aeromonas |
| topic | aeromonas; biological control; cyprinus carpio; intestinal bacteria |
| url | https://microbiologyjournal.org/intestinal-bacteria-of-common-carp-cyprinus-carpio-l-as-a-biological-control-agent-for-aeromonas/ |
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