The involvement of high succinylation modification in the development of prostate cancer
ObjectiveSuccinylation modification of the lysine site plays an important role in tumorigenesis and development, but it is rarely reported in prostate cancer (PCa), so this study aims to elucidate its expression in and clinical correlation with PCa.MethodsA total of 95 tumor, 3 normal and 52 paired...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.1034605/full |
| _version_ | 1797989516564234240 |
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| author | Zhenyang Zhang Zhenyang Zhang Yanru Chen Yanru Chen Lingyu Fang Lingyu Fang Jiang Zhao Shishan Deng Shishan Deng |
| author_facet | Zhenyang Zhang Zhenyang Zhang Yanru Chen Yanru Chen Lingyu Fang Lingyu Fang Jiang Zhao Shishan Deng Shishan Deng |
| author_sort | Zhenyang Zhang |
| collection | DOAJ |
| description | ObjectiveSuccinylation modification of the lysine site plays an important role in tumorigenesis and development, but it is rarely reported in prostate cancer (PCa), so this study aims to elucidate its expression in and clinical correlation with PCa.MethodsA total of 95 tumor, 3 normal and 52 paired adjacent tissue of PCa were involved for succinylation stanning. 498 PCa samples with 20 succinylation modification-related genes from TCGA were downloaded for model construction. Statistical methods were employed to analyze the data, including Non-Negative Matrix Factorization (NMF) algorithm, t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm and Cox regression analysis.ResultsThe pan-succinyllysine antibody stanning indicated that tumor tissues showed higher succinyllysine level than adjacent tissues (p<0.001). Gleason grade and PDL1 expression levels were significantly different (p<0.001) among the high, medium and low succinylation staining scores. The types of PCa tissue were divided into four clusters using RNA-seq data of 20 succinylation-related genes in TCGA database. Clinical characterize of age, PSA level, and pathological stage showed differences among four clusters. The expression of succinylation-related genes (KAT5, SDHD and GLYATL1) and PCa related genes (PDL1, AR and TP53) were significantly different in 52 matched tumor and adjacent tissues (p<0.001). GLYATL1 and AR gene expression was significantly related to the pathological stage of PCa.ConclusionSuccinylation was significantly increased in PCa tissues and was closely related to Gleason grade and PD-L1 expression. Model construction of 20 genes related to succinylation modification showed that the later the pathological stage of PCa, the higher the level of succinylation modification. |
| first_indexed | 2024-04-11T08:21:36Z |
| format | Article |
| id | doaj.art-a6d13091131549718a8e4b98408409f0 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-11T08:21:36Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-a6d13091131549718a8e4b98408409f02022-12-22T04:34:57ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-11-011210.3389/fonc.2022.10346051034605The involvement of high succinylation modification in the development of prostate cancerZhenyang Zhang0Zhenyang Zhang1Yanru Chen2Yanru Chen3Lingyu Fang4Lingyu Fang5Jiang Zhao6Shishan Deng7Shishan Deng8Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, ChinaBreast Cancer Biological Targeting Institute, North Sichuan Medical College, Nanchong, Sichuan, ChinaInstitute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, ChinaBreast Cancer Biological Targeting Institute, North Sichuan Medical College, Nanchong, Sichuan, ChinaInstitute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, ChinaBreast Cancer Biological Targeting Institute, North Sichuan Medical College, Nanchong, Sichuan, ChinaDepartment of Urology, Second Affiliated Hospital, Army Military Medical University, Chongqing, ChinaInstitute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, ChinaBreast Cancer Biological Targeting Institute, North Sichuan Medical College, Nanchong, Sichuan, ChinaObjectiveSuccinylation modification of the lysine site plays an important role in tumorigenesis and development, but it is rarely reported in prostate cancer (PCa), so this study aims to elucidate its expression in and clinical correlation with PCa.MethodsA total of 95 tumor, 3 normal and 52 paired adjacent tissue of PCa were involved for succinylation stanning. 498 PCa samples with 20 succinylation modification-related genes from TCGA were downloaded for model construction. Statistical methods were employed to analyze the data, including Non-Negative Matrix Factorization (NMF) algorithm, t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm and Cox regression analysis.ResultsThe pan-succinyllysine antibody stanning indicated that tumor tissues showed higher succinyllysine level than adjacent tissues (p<0.001). Gleason grade and PDL1 expression levels were significantly different (p<0.001) among the high, medium and low succinylation staining scores. The types of PCa tissue were divided into four clusters using RNA-seq data of 20 succinylation-related genes in TCGA database. Clinical characterize of age, PSA level, and pathological stage showed differences among four clusters. The expression of succinylation-related genes (KAT5, SDHD and GLYATL1) and PCa related genes (PDL1, AR and TP53) were significantly different in 52 matched tumor and adjacent tissues (p<0.001). GLYATL1 and AR gene expression was significantly related to the pathological stage of PCa.ConclusionSuccinylation was significantly increased in PCa tissues and was closely related to Gleason grade and PD-L1 expression. Model construction of 20 genes related to succinylation modification showed that the later the pathological stage of PCa, the higher the level of succinylation modification.https://www.frontiersin.org/articles/10.3389/fonc.2022.1034605/fullprostate cancersuccinylationpost-translational modificationGLYATL1PDL1 |
| spellingShingle | Zhenyang Zhang Zhenyang Zhang Yanru Chen Yanru Chen Lingyu Fang Lingyu Fang Jiang Zhao Shishan Deng Shishan Deng The involvement of high succinylation modification in the development of prostate cancer Frontiers in Oncology prostate cancer succinylation post-translational modification GLYATL1 PDL1 |
| title | The involvement of high succinylation modification in the development of prostate cancer |
| title_full | The involvement of high succinylation modification in the development of prostate cancer |
| title_fullStr | The involvement of high succinylation modification in the development of prostate cancer |
| title_full_unstemmed | The involvement of high succinylation modification in the development of prostate cancer |
| title_short | The involvement of high succinylation modification in the development of prostate cancer |
| title_sort | involvement of high succinylation modification in the development of prostate cancer |
| topic | prostate cancer succinylation post-translational modification GLYATL1 PDL1 |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.1034605/full |
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