Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia
Primary ciliary dyskinesia (PCD) is a disorder that affects motile cilia in the airway that are required for the removal of mucus, debris, and pathogens. It is important to diagnose PCD in early childhood to preserve lung function. The confirmation of a diagnosis relies on the assessment of ciliary...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/10/8/524 |
| _version_ | 1797561089635909632 |
|---|---|
| author | Andreia L. Pinto Ranjit K. Rai Claire Hogg Thomas Burgoyne |
| author_facet | Andreia L. Pinto Ranjit K. Rai Claire Hogg Thomas Burgoyne |
| author_sort | Andreia L. Pinto |
| collection | DOAJ |
| description | Primary ciliary dyskinesia (PCD) is a disorder that affects motile cilia in the airway that are required for the removal of mucus, debris, and pathogens. It is important to diagnose PCD in early childhood to preserve lung function. The confirmation of a diagnosis relies on the assessment of ciliary ultrastructure by transmission electron microscopy (TEM). TEM involves the quantitative assessment of the ciliary ultrastructure to identify PCD defects as well as abnormalities resulting from infection. Many specialist diagnostic centres still rely on physical counters to tally results and paper notes to summarise findings before transferring the results to computer databases/records. To speed up the diagnostic data collection and increase the protection of patient information, we have developed digital ciliary feature counters that conform to the PCD reporting international consensus guideline. These counters can be used on a computer or tablet, and automatically generate notes regarding sample observations. We show that the digital counters are easy to use and can generate TEM diagnostic reports that will be useful for many PCD diagnostic centres. |
| first_indexed | 2024-03-10T18:09:20Z |
| format | Article |
| id | doaj.art-a6d57c22736649728b22e872b65c293b |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T18:09:20Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a6d57c22736649728b22e872b65c293b2023-11-20T08:14:13ZengMDPI AGDiagnostics2075-44182020-07-0110852410.3390/diagnostics10080524Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary DyskinesiaAndreia L. Pinto0Ranjit K. Rai1Claire Hogg2Thomas Burgoyne3Paediatric Respiratory Medicine, Department of Paediatrics, Royal Brompton & Harefield NHS Trust, London SW3 6NP, UKPaediatric Respiratory Medicine, Department of Paediatrics, Royal Brompton & Harefield NHS Trust, London SW3 6NP, UKPaediatric Respiratory Medicine, Department of Paediatrics, Royal Brompton & Harefield NHS Trust, London SW3 6NP, UKPaediatric Respiratory Medicine, Department of Paediatrics, Royal Brompton & Harefield NHS Trust, London SW3 6NP, UKPrimary ciliary dyskinesia (PCD) is a disorder that affects motile cilia in the airway that are required for the removal of mucus, debris, and pathogens. It is important to diagnose PCD in early childhood to preserve lung function. The confirmation of a diagnosis relies on the assessment of ciliary ultrastructure by transmission electron microscopy (TEM). TEM involves the quantitative assessment of the ciliary ultrastructure to identify PCD defects as well as abnormalities resulting from infection. Many specialist diagnostic centres still rely on physical counters to tally results and paper notes to summarise findings before transferring the results to computer databases/records. To speed up the diagnostic data collection and increase the protection of patient information, we have developed digital ciliary feature counters that conform to the PCD reporting international consensus guideline. These counters can be used on a computer or tablet, and automatically generate notes regarding sample observations. We show that the digital counters are easy to use and can generate TEM diagnostic reports that will be useful for many PCD diagnostic centres.https://www.mdpi.com/2075-4418/10/8/524ciliaprimary ciliary dyskinesiadigital counter |
| spellingShingle | Andreia L. Pinto Ranjit K. Rai Claire Hogg Thomas Burgoyne Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia Diagnostics cilia primary ciliary dyskinesia digital counter |
| title | Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia |
| title_full | Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia |
| title_fullStr | Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia |
| title_full_unstemmed | Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia |
| title_short | Ciliary Feature Counter: A program for the Quantitative Assessment of Cilia to Diagnose Primary Ciliary Dyskinesia |
| title_sort | ciliary feature counter a program for the quantitative assessment of cilia to diagnose primary ciliary dyskinesia |
| topic | cilia primary ciliary dyskinesia digital counter |
| url | https://www.mdpi.com/2075-4418/10/8/524 |
| work_keys_str_mv | AT andreialpinto ciliaryfeaturecounteraprogramforthequantitativeassessmentofciliatodiagnoseprimaryciliarydyskinesia AT ranjitkrai ciliaryfeaturecounteraprogramforthequantitativeassessmentofciliatodiagnoseprimaryciliarydyskinesia AT clairehogg ciliaryfeaturecounteraprogramforthequantitativeassessmentofciliatodiagnoseprimaryciliarydyskinesia AT thomasburgoyne ciliaryfeaturecounteraprogramforthequantitativeassessmentofciliatodiagnoseprimaryciliarydyskinesia |