Multiple airy beams light-sheet fluorescence microscopy
Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field of view of Light-sheet fluorescence microscopy benefiting from its non-diffracti...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
|
| Series: | Frontiers in Physics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphy.2022.1111023/full |
| _version_ | 1828088558642003968 |
|---|---|
| author | Shuangyu Gu Shuangyu Gu Xianghua Yu Xianghua Yu Chen Bai Junwei Min Runze Li Yanlong Yang Baoli Yao Baoli Yao |
| author_facet | Shuangyu Gu Shuangyu Gu Xianghua Yu Xianghua Yu Chen Bai Junwei Min Runze Li Yanlong Yang Baoli Yao Baoli Yao |
| author_sort | Shuangyu Gu |
| collection | DOAJ |
| description | Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field of view of Light-sheet fluorescence microscopy benefiting from its non-diffracting nature, but at the cost of out-of-focus background and low imaging contrast caused by side lobes illumination. Here, we propose a method to generate a sort of multiple Airy beams (MAB), which are linearly superimposed of multiple single Airy beams with different scale factors. Compared to the SAB light-sheet, the energy of the multiple Airy beams light-sheet is more concentrated on the focal plane of the detection objective, which can improve the imaging contrast and decrease the photodamage effect. Furthermore, we combined the complementary beam subtraction (CBS) strategy to increase the axial resolution, termed as multiple Airy beams-complementary beam subtraction method, which enables the axial resolution of 1.2 μm while keeping the field of view of 450 μm × 450 μm. The effectiveness of the method is demonstrated by imaging of fluorescent beads and aspergillus conidiophores. |
| first_indexed | 2024-04-11T05:27:23Z |
| format | Article |
| id | doaj.art-a6dbdbfe611940979abd503614f9d712 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-04-11T05:27:23Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-a6dbdbfe611940979abd503614f9d7122022-12-23T05:06:03ZengFrontiers Media S.A.Frontiers in Physics2296-424X2022-12-011010.3389/fphy.2022.11110231111023Multiple airy beams light-sheet fluorescence microscopyShuangyu Gu0Shuangyu Gu1Xianghua Yu2Xianghua Yu3Chen Bai4Junwei Min5Runze Li6Yanlong Yang7Baoli Yao8Baoli Yao9State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaState Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaLight-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field of view of Light-sheet fluorescence microscopy benefiting from its non-diffracting nature, but at the cost of out-of-focus background and low imaging contrast caused by side lobes illumination. Here, we propose a method to generate a sort of multiple Airy beams (MAB), which are linearly superimposed of multiple single Airy beams with different scale factors. Compared to the SAB light-sheet, the energy of the multiple Airy beams light-sheet is more concentrated on the focal plane of the detection objective, which can improve the imaging contrast and decrease the photodamage effect. Furthermore, we combined the complementary beam subtraction (CBS) strategy to increase the axial resolution, termed as multiple Airy beams-complementary beam subtraction method, which enables the axial resolution of 1.2 μm while keeping the field of view of 450 μm × 450 μm. The effectiveness of the method is demonstrated by imaging of fluorescent beads and aspergillus conidiophores.https://www.frontiersin.org/articles/10.3389/fphy.2022.1111023/fulllight-sheet fluorescence microscopyvolumetric imagingnon-diffracting beamimage contrastfield of view |
| spellingShingle | Shuangyu Gu Shuangyu Gu Xianghua Yu Xianghua Yu Chen Bai Junwei Min Runze Li Yanlong Yang Baoli Yao Baoli Yao Multiple airy beams light-sheet fluorescence microscopy Frontiers in Physics light-sheet fluorescence microscopy volumetric imaging non-diffracting beam image contrast field of view |
| title | Multiple airy beams light-sheet fluorescence microscopy |
| title_full | Multiple airy beams light-sheet fluorescence microscopy |
| title_fullStr | Multiple airy beams light-sheet fluorescence microscopy |
| title_full_unstemmed | Multiple airy beams light-sheet fluorescence microscopy |
| title_short | Multiple airy beams light-sheet fluorescence microscopy |
| title_sort | multiple airy beams light sheet fluorescence microscopy |
| topic | light-sheet fluorescence microscopy volumetric imaging non-diffracting beam image contrast field of view |
| url | https://www.frontiersin.org/articles/10.3389/fphy.2022.1111023/full |
| work_keys_str_mv | AT shuangyugu multipleairybeamslightsheetfluorescencemicroscopy AT shuangyugu multipleairybeamslightsheetfluorescencemicroscopy AT xianghuayu multipleairybeamslightsheetfluorescencemicroscopy AT xianghuayu multipleairybeamslightsheetfluorescencemicroscopy AT chenbai multipleairybeamslightsheetfluorescencemicroscopy AT junweimin multipleairybeamslightsheetfluorescencemicroscopy AT runzeli multipleairybeamslightsheetfluorescencemicroscopy AT yanlongyang multipleairybeamslightsheetfluorescencemicroscopy AT baoliyao multipleairybeamslightsheetfluorescencemicroscopy AT baoliyao multipleairybeamslightsheetfluorescencemicroscopy |