Cytotoxic activity of the aqueous extract of Micromeria fruticosa (L.) Druce subsp. serpyllifolia on human U-87 MG cell lines
Micromeria fruticosa (L.) Druce subsp. serpyllifolia, which is widely used in folk medicine as a medicinal herbal tea, is grown in different areas of Turkey and the Mediterranean region. The present study was conducted to evaluate the aqueous extract of Micromeria fruticosa subsp. serpyllif...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600119K.pdf |
| Summary: | Micromeria fruticosa (L.) Druce subsp. serpyllifolia, which is widely used in
folk medicine as a medicinal herbal tea, is grown in different areas of
Turkey and the Mediterranean region. The present study was conducted to
evaluate the aqueous extract of Micromeria fruticosa subsp. serpyllifolia for
its antioxidant and antiproliferative activity on a human glioblastoma
multiforme cell line (U-87 MG), which has not been reported before. Here, the
extract was added to cultures at 8 different concentrations (0-200 μg/mL).
Cell viability and cell membrane damage was determined using the MTT and LDH
assays for 48 h, respectively. To examine the oxidative effects, total
antioxidant capacity (TAC) and total oxidant status (TOS) levels were
measured. The extract displayed considerable antiproliferative activities at
the high concentrations of 175 and 200 μg/mL. Furthermore, the extract caused
a significant increase in the release of the lactate dehydrogenase (LDH)
enzyme in a concentration-dependent manner; 200 μg/mL of extract enhanced the
release of LDH. Treatments with extract at higher doses increased TOS levels
and decreased TAC levels in human U-87 MG cells. Our study suggests that the
aqueous extract of Micromeria fruticosa ssp. serpyllifolia was capable of
inducing growth inhibition of cancer cells. These results encourage further
research to assess the value of the extract in modern phytotherapy. |
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| ISSN: | 0354-4664 1821-4339 |