Does dentine mineral change with anatomical location, microscopic site and patient age?
Objective: To determine the effect of patient age (young or mature), anatomical location (shallow/deep and central/peripheral) and microscopic site (intertubular/peritubular) on dentine mineral density, distribution and composition. Methods: Extracted posterior teeth from young (aged 19–20 years, N ...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of Structural Biology: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590152422000010 |
| _version_ | 1811178589202153472 |
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| author | Arosha T. Weerakoon Crystal Cooper Ian A. Meyers Nicholas Condon Christopher Sexton David Thomson Pauline J. Ford Anne L. Symons |
| author_facet | Arosha T. Weerakoon Crystal Cooper Ian A. Meyers Nicholas Condon Christopher Sexton David Thomson Pauline J. Ford Anne L. Symons |
| author_sort | Arosha T. Weerakoon |
| collection | DOAJ |
| description | Objective: To determine the effect of patient age (young or mature), anatomical location (shallow/deep and central/peripheral) and microscopic site (intertubular/peritubular) on dentine mineral density, distribution and composition. Methods: Extracted posterior teeth from young (aged 19–20 years, N = 4) and mature (aged 54–77 years, N = 4) subjects were prepared to shallow and deep slices. The dentine surface elemental composition was investigated in a SEM using Backscattered Electron (BSE) micrographs, Energy Dispersive X-ray Spectroscopy, and Integrated Mineral Analysis. Qualitative comparisons and quantitative measures using machine learning were used to analyse the BSE images. Quantitative outcomes were compared using quantile or linear regression models with bootstrapping to account for the multiple measures per sample. Subsequently, a Xenon Plasma Focussed Ion Beam Scanning Electron Microscopy (Xe PFIB-SEM) was used to mill large area (100 µm) cross-sections to investigate morphology through the dentine tubules using high resolution secondary electron micrographs. Results: With age, dentine mineral composition remains stable, but density changes with anatomical location and microscopic site. Microscopically, accessory tubules spread into intertubular dentine (ITD) from the main tubule lumens. Within the lumens, mineral deposits form calcospherites in the young that eventually coalesce in mature tubules and branches. The mineral occlusion in mature dentine increases overall ITD density to reflect peritubular dentine (PTD) infiltrate. The ITD observed in micrographs remained consistent for age and observation plane to suggest tubule deposition affects overall dentine density. Mineral density depends on the relative distribution of PTD to ITD that varies with anatomical location. Significance: Adhesive materials may interact differently within a tooth as well as in different age groups. |
| first_indexed | 2024-04-11T06:20:57Z |
| format | Article |
| id | doaj.art-a6e01b9b64af449287e1d145cbd0a774 |
| institution | Directory Open Access Journal |
| issn | 2590-1524 |
| language | English |
| last_indexed | 2024-04-11T06:20:57Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Structural Biology: X |
| spelling | doaj.art-a6e01b9b64af449287e1d145cbd0a7742022-12-22T04:40:34ZengElsevierJournal of Structural Biology: X2590-15242022-01-016100060Does dentine mineral change with anatomical location, microscopic site and patient age?Arosha T. Weerakoon0Crystal Cooper1Ian A. Meyers2Nicholas Condon3Christopher Sexton4David Thomson5Pauline J. Ford6Anne L. Symons7School of Dentistry, The University of Queensland, Brisbane, Queensland, Australia; Corresponding author at: Oral Health Centre, Herston Road, Herston, School of Dentistry, The University of Queensland, Brisbane, QLD 4006, Australia.Central Analytical Research Facility, Queensland University of Technology, Brisbane, Queensland, AustraliaSchool of Dentistry, The University of Queensland, Brisbane, Queensland, AustraliaInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, AustraliaSchool of Dentistry, The University of Queensland, Brisbane, Queensland, AustraliaSchool of Dentistry, The University of Queensland, Brisbane, Queensland, AustraliaSchool of Dentistry, The University of Queensland, Brisbane, Queensland, AustraliaSchool of Dentistry, The University of Queensland, Brisbane, Queensland, AustraliaObjective: To determine the effect of patient age (young or mature), anatomical location (shallow/deep and central/peripheral) and microscopic site (intertubular/peritubular) on dentine mineral density, distribution and composition. Methods: Extracted posterior teeth from young (aged 19–20 years, N = 4) and mature (aged 54–77 years, N = 4) subjects were prepared to shallow and deep slices. The dentine surface elemental composition was investigated in a SEM using Backscattered Electron (BSE) micrographs, Energy Dispersive X-ray Spectroscopy, and Integrated Mineral Analysis. Qualitative comparisons and quantitative measures using machine learning were used to analyse the BSE images. Quantitative outcomes were compared using quantile or linear regression models with bootstrapping to account for the multiple measures per sample. Subsequently, a Xenon Plasma Focussed Ion Beam Scanning Electron Microscopy (Xe PFIB-SEM) was used to mill large area (100 µm) cross-sections to investigate morphology through the dentine tubules using high resolution secondary electron micrographs. Results: With age, dentine mineral composition remains stable, but density changes with anatomical location and microscopic site. Microscopically, accessory tubules spread into intertubular dentine (ITD) from the main tubule lumens. Within the lumens, mineral deposits form calcospherites in the young that eventually coalesce in mature tubules and branches. The mineral occlusion in mature dentine increases overall ITD density to reflect peritubular dentine (PTD) infiltrate. The ITD observed in micrographs remained consistent for age and observation plane to suggest tubule deposition affects overall dentine density. Mineral density depends on the relative distribution of PTD to ITD that varies with anatomical location. Significance: Adhesive materials may interact differently within a tooth as well as in different age groups.http://www.sciencedirect.com/science/article/pii/S2590152422000010DentineMineralApatiteBSESEM-EDSXe PFIB-SEM |
| spellingShingle | Arosha T. Weerakoon Crystal Cooper Ian A. Meyers Nicholas Condon Christopher Sexton David Thomson Pauline J. Ford Anne L. Symons Does dentine mineral change with anatomical location, microscopic site and patient age? Journal of Structural Biology: X Dentine Mineral Apatite BSE SEM-EDS Xe PFIB-SEM |
| title | Does dentine mineral change with anatomical location, microscopic site and patient age? |
| title_full | Does dentine mineral change with anatomical location, microscopic site and patient age? |
| title_fullStr | Does dentine mineral change with anatomical location, microscopic site and patient age? |
| title_full_unstemmed | Does dentine mineral change with anatomical location, microscopic site and patient age? |
| title_short | Does dentine mineral change with anatomical location, microscopic site and patient age? |
| title_sort | does dentine mineral change with anatomical location microscopic site and patient age |
| topic | Dentine Mineral Apatite BSE SEM-EDS Xe PFIB-SEM |
| url | http://www.sciencedirect.com/science/article/pii/S2590152422000010 |
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