Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i>
Banana (<i>Musa</i> spp.), which is one of the world’s most popular and most traded fruits, is highly susceptible to pests and diseases. <i>Pseudocercospora musae</i>, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of <i>Musa</i> spp.,...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13589 |
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| author | Tatiana David Miranda Pinheiro Erica Cristina Silva Rego Gabriel Sergio Costa Alves Fernando Campos De Assis Fonseca Michelle Guitton Cotta Jose Dijair Antonino Taísa Godoy Gomes Edson Perito Amorim Claudia Fortes Ferreira Marcos Mota Do Carmo Costa Priscila Grynberg Roberto Coiti Togawa Robert Neil Gerard Miller |
| author_facet | Tatiana David Miranda Pinheiro Erica Cristina Silva Rego Gabriel Sergio Costa Alves Fernando Campos De Assis Fonseca Michelle Guitton Cotta Jose Dijair Antonino Taísa Godoy Gomes Edson Perito Amorim Claudia Fortes Ferreira Marcos Mota Do Carmo Costa Priscila Grynberg Roberto Coiti Togawa Robert Neil Gerard Miller |
| author_sort | Tatiana David Miranda Pinheiro |
| collection | DOAJ |
| description | Banana (<i>Musa</i> spp.), which is one of the world’s most popular and most traded fruits, is highly susceptible to pests and diseases. <i>Pseudocercospora musae</i>, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of <i>Musa</i> spp., resulting in serious economic damage to cultivars in the Cavendish subgroup. The aim of this study was to characterize genetic components of the early immune response to <i>P. musae</i> in <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4, a resistant wild diploid. Leaf RNA samples were extracted from Calcutta 4 three days after inoculation with fungal conidiospores, with paired-end sequencing conducted in inoculated and non-inoculated controls using lllumina HiSeq 4000 technology. Following mapping to the reference <i>M. acuminata</i> ssp. <i>malaccensis</i> var. Pahang genome, differentially expressed genes (DEGs) were identified and expression representation analyzed on the basis of gene ontology enrichment, Kyoto Encyclopedia of Genes and Genomes orthology and MapMan pathway analysis. Sequence data mapped to 29,757 gene transcript models in the reference <i>Musa</i> genome. A total of 1073 DEGs were identified in pathogen-inoculated cDNA libraries, in comparison to non-inoculated controls, with 32% overexpressed. GO enrichment analysis revealed common assignment to terms that included chitin binding, chitinase activity, pattern binding, oxidoreductase activity and transcription factor (TF) activity. Allocation to KEGG pathways revealed DEGs associated with environmental information processing, signaling, biosynthesis of secondary metabolites, and metabolism of terpenoids and polyketides. With 144 up-regulated DEGs potentially involved in biotic stress response pathways, including genes involved in cell wall reinforcement, PTI responses, TF regulation, phytohormone signaling and secondary metabolism, data demonstrated diverse early-stage defense responses to <i>P. musae</i>. With increased understanding of the defense responses occurring during the incompatible interaction in resistant Calcutta 4, these data are appropriate for the development of effective disease management approaches based on genetic improvement through introgression of candidate genes in superior cultivars. |
| first_indexed | 2024-03-09T18:59:50Z |
| format | Article |
| id | doaj.art-a6e4079051694754b4d166625d9bb855 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a6e4079051694754b4d166625d9bb8552023-11-24T05:10:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211358910.3390/ijms232113589Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i>Tatiana David Miranda Pinheiro0Erica Cristina Silva Rego1Gabriel Sergio Costa Alves2Fernando Campos De Assis Fonseca3Michelle Guitton Cotta4Jose Dijair Antonino5Taísa Godoy Gomes6Edson Perito Amorim7Claudia Fortes Ferreira8Marcos Mota Do Carmo Costa9Priscila Grynberg10Roberto Coiti Togawa11Robert Neil Gerard Miller12Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilInstituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilInstituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilDepartament of Academic Areas, Instituto Federal de Goiás (IFG), Águas Lindas 72910-733, BrazilInstituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilDepartamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Recife 52171-900, BrazilInstituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilEmbrapa Cassava and Tropical Fruits, Cruz das Almas 44380-000, BrazilEmbrapa Cassava and Tropical Fruits, Cruz das Almas 44380-000, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-917, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-917, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília 70770-917, BrazilInstituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, BrazilBanana (<i>Musa</i> spp.), which is one of the world’s most popular and most traded fruits, is highly susceptible to pests and diseases. <i>Pseudocercospora musae</i>, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of <i>Musa</i> spp., resulting in serious economic damage to cultivars in the Cavendish subgroup. The aim of this study was to characterize genetic components of the early immune response to <i>P. musae</i> in <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4, a resistant wild diploid. Leaf RNA samples were extracted from Calcutta 4 three days after inoculation with fungal conidiospores, with paired-end sequencing conducted in inoculated and non-inoculated controls using lllumina HiSeq 4000 technology. Following mapping to the reference <i>M. acuminata</i> ssp. <i>malaccensis</i> var. Pahang genome, differentially expressed genes (DEGs) were identified and expression representation analyzed on the basis of gene ontology enrichment, Kyoto Encyclopedia of Genes and Genomes orthology and MapMan pathway analysis. Sequence data mapped to 29,757 gene transcript models in the reference <i>Musa</i> genome. A total of 1073 DEGs were identified in pathogen-inoculated cDNA libraries, in comparison to non-inoculated controls, with 32% overexpressed. GO enrichment analysis revealed common assignment to terms that included chitin binding, chitinase activity, pattern binding, oxidoreductase activity and transcription factor (TF) activity. Allocation to KEGG pathways revealed DEGs associated with environmental information processing, signaling, biosynthesis of secondary metabolites, and metabolism of terpenoids and polyketides. With 144 up-regulated DEGs potentially involved in biotic stress response pathways, including genes involved in cell wall reinforcement, PTI responses, TF regulation, phytohormone signaling and secondary metabolism, data demonstrated diverse early-stage defense responses to <i>P. musae</i>. With increased understanding of the defense responses occurring during the incompatible interaction in resistant Calcutta 4, these data are appropriate for the development of effective disease management approaches based on genetic improvement through introgression of candidate genes in superior cultivars.https://www.mdpi.com/1422-0067/23/21/13589<i>Musa acuminata</i><i>Pseudocercospora musae</i>Sigatoka leaf spotbiotic stressdisease resistancetranscriptome |
| spellingShingle | Tatiana David Miranda Pinheiro Erica Cristina Silva Rego Gabriel Sergio Costa Alves Fernando Campos De Assis Fonseca Michelle Guitton Cotta Jose Dijair Antonino Taísa Godoy Gomes Edson Perito Amorim Claudia Fortes Ferreira Marcos Mota Do Carmo Costa Priscila Grynberg Roberto Coiti Togawa Robert Neil Gerard Miller Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> International Journal of Molecular Sciences <i>Musa acuminata</i> <i>Pseudocercospora musae</i> Sigatoka leaf spot biotic stress disease resistance transcriptome |
| title | Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> |
| title_full | Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> |
| title_fullStr | Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> |
| title_full_unstemmed | Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> |
| title_short | Transcriptome Profiling of the Resistance Response of <i>Musa acuminata</i> subsp. <i>burmannicoides</i>, var. Calcutta 4 to <i>Pseudocercospora musae</i> |
| title_sort | transcriptome profiling of the resistance response of i musa acuminata i subsp i burmannicoides i var calcutta 4 to i pseudocercospora musae i |
| topic | <i>Musa acuminata</i> <i>Pseudocercospora musae</i> Sigatoka leaf spot biotic stress disease resistance transcriptome |
| url | https://www.mdpi.com/1422-0067/23/21/13589 |
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