Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis
The plant hormone ABA (abscisic acid) regulates plant responses to abiotic stresses by regulating the expression of ABA response genes. However, the functions of a large portion of ABA response genes have remained unclear. We report in this study the identification of ASDs (ABA-inducible signal pept...
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MDPI AG
2023-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/16/2989 |
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| author | Yingying Li Wei Wang Na Zhang Yuxin Cheng Saddam Hussain Yating Wang Hainan Tian Hadia Hussain Rao Lin Yuan Yuan Chen Wang Tianya Wang Shucai Wang |
| author_facet | Yingying Li Wei Wang Na Zhang Yuxin Cheng Saddam Hussain Yating Wang Hainan Tian Hadia Hussain Rao Lin Yuan Yuan Chen Wang Tianya Wang Shucai Wang |
| author_sort | Yingying Li |
| collection | DOAJ |
| description | The plant hormone ABA (abscisic acid) regulates plant responses to abiotic stresses by regulating the expression of ABA response genes. However, the functions of a large portion of ABA response genes have remained unclear. We report in this study the identification of ASDs (ABA-inducible signal peptide-containing DUF538 proteins), a subgroup of DUF538 proteins with a signal peptide, as the regulators of plant responses to ABA in Arabidopsis. ASDs are encoded by four closely related DUF538 genes, with <i>ASD1</i>/<i>ASD2</i> and <i>ASD3</i>/<i>ASD4</i> being two pairs of duplicated tandemly repeated genes. The quantitative RT-PCR (qRT-PCR) results showed that the expression levels of <i>ASDs</i> increased significantly in response to ABA as well as NaCl and mannitol treatments, with the exception that the expression level of <i>ASD2</i> remained largely unchanged in response to NaCl treatment. The results of Arabidopsis protoplast transient transfection assays showed that ASDs were localized on the plasma membrane and in the cytosol and nucleus. When recruited to the promoter of the reporter gene via a fused GD domain, ASDs were able to slightly repress the expression of the co-transfected reporter gene. Seed germination and cotyledon greening assays showed that ABA sensitivity was increased in the transgenic plants that were over-expressing <i>ASD1</i> or <i>ASD3</i> but decreased in the transgenic plants that were over-expressing <i>ASD2</i> or <i>ASD4</i>. On the other hand, ABA sensitivity was increased in the CRISPR/Cas9 gene-edited <i>asd2</i> single mutants but decreased in the <i>asd3</i> single mutants. A transcriptome analysis showed that differentially expressed genes in the <i>35S:ASD2</i> transgenic plant seedlings were enriched in several different processes, including in plant growth and development, the secondary metabolism, and plant hormone signaling. In summary, our results show that <i>ASDs</i> are ABA response genes and that ASDs are involved in the regulation of plant responses to ABA in Arabidopsis; however, ASD1/ASD3 and ASD2/ASD4 have opposite functions. |
| first_indexed | 2024-03-10T23:38:49Z |
| format | Article |
| id | doaj.art-a6e6cc6dba77408eb80ff40226d50770 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T23:38:49Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-a6e6cc6dba77408eb80ff40226d507702023-11-19T02:42:04ZengMDPI AGPlants2223-77472023-08-011216298910.3390/plants12162989Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in ArabidopsisYingying Li0Wei Wang1Na Zhang2Yuxin Cheng3Saddam Hussain4Yating Wang5Hainan Tian6Hadia Hussain7Rao Lin8Yuan Yuan9Chen Wang10Tianya Wang11Shucai Wang12Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaLaboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi 276000, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaLaboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi 276000, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaKey Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, ChinaThe plant hormone ABA (abscisic acid) regulates plant responses to abiotic stresses by regulating the expression of ABA response genes. However, the functions of a large portion of ABA response genes have remained unclear. We report in this study the identification of ASDs (ABA-inducible signal peptide-containing DUF538 proteins), a subgroup of DUF538 proteins with a signal peptide, as the regulators of plant responses to ABA in Arabidopsis. ASDs are encoded by four closely related DUF538 genes, with <i>ASD1</i>/<i>ASD2</i> and <i>ASD3</i>/<i>ASD4</i> being two pairs of duplicated tandemly repeated genes. The quantitative RT-PCR (qRT-PCR) results showed that the expression levels of <i>ASDs</i> increased significantly in response to ABA as well as NaCl and mannitol treatments, with the exception that the expression level of <i>ASD2</i> remained largely unchanged in response to NaCl treatment. The results of Arabidopsis protoplast transient transfection assays showed that ASDs were localized on the plasma membrane and in the cytosol and nucleus. When recruited to the promoter of the reporter gene via a fused GD domain, ASDs were able to slightly repress the expression of the co-transfected reporter gene. Seed germination and cotyledon greening assays showed that ABA sensitivity was increased in the transgenic plants that were over-expressing <i>ASD1</i> or <i>ASD3</i> but decreased in the transgenic plants that were over-expressing <i>ASD2</i> or <i>ASD4</i>. On the other hand, ABA sensitivity was increased in the CRISPR/Cas9 gene-edited <i>asd2</i> single mutants but decreased in the <i>asd3</i> single mutants. A transcriptome analysis showed that differentially expressed genes in the <i>35S:ASD2</i> transgenic plant seedlings were enriched in several different processes, including in plant growth and development, the secondary metabolism, and plant hormone signaling. In summary, our results show that <i>ASDs</i> are ABA response genes and that ASDs are involved in the regulation of plant responses to ABA in Arabidopsis; however, ASD1/ASD3 and ASD2/ASD4 have opposite functions.https://www.mdpi.com/2223-7747/12/16/2989ASDsABADUF538 proteinsCRISPR/Cas9Arabidopsis |
| spellingShingle | Yingying Li Wei Wang Na Zhang Yuxin Cheng Saddam Hussain Yating Wang Hainan Tian Hadia Hussain Rao Lin Yuan Yuan Chen Wang Tianya Wang Shucai Wang Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis Plants ASDs ABA DUF538 proteins CRISPR/Cas9 Arabidopsis |
| title | Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis |
| title_full | Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis |
| title_fullStr | Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis |
| title_full_unstemmed | Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis |
| title_short | Antagonistic Regulation of ABA Responses by Duplicated Tandemly Repeated DUF538 Protein Genes in Arabidopsis |
| title_sort | antagonistic regulation of aba responses by duplicated tandemly repeated duf538 protein genes in arabidopsis |
| topic | ASDs ABA DUF538 proteins CRISPR/Cas9 Arabidopsis |
| url | https://www.mdpi.com/2223-7747/12/16/2989 |
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