Dual functions of TAF7L in adipocyte differentiation
The diverse transcriptional mechanisms governing cellular differentiation and development of mammalian tissue remains poorly understood. Here we report that TAF7L, a paralogue of TFIID subunit TAF7, is enriched in adipocytes and white fat tissue (WAT) in mouse. Depletion of TAF7L reduced adipocyte-s...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2013-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00170 |
| _version_ | 1811201075761381376 |
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| author | Haiying Zhou Tommy Kaplan Yan Li Ivan Grubisic Zhengjian Zhang P Jeremy Wang Michael B Eisen Robert Tjian |
| author_facet | Haiying Zhou Tommy Kaplan Yan Li Ivan Grubisic Zhengjian Zhang P Jeremy Wang Michael B Eisen Robert Tjian |
| author_sort | Haiying Zhou |
| collection | DOAJ |
| description | The diverse transcriptional mechanisms governing cellular differentiation and development of mammalian tissue remains poorly understood. Here we report that TAF7L, a paralogue of TFIID subunit TAF7, is enriched in adipocytes and white fat tissue (WAT) in mouse. Depletion of TAF7L reduced adipocyte-specific gene expression, compromised adipocyte differentiation, and WAT development as well. Ectopic expression of TAF7L in myoblasts reprograms these muscle precursors into adipocytes upon induction. Genome-wide mRNA-seq expression profiling and ChIP-seq binding studies confirmed that TAF7L is required for activating adipocyte-specific genes via a dual mechanism wherein it interacts with PPARγ at enhancers and TBP/Pol II at core promoters. In vitro binding studies confirmed that TAF7L forms complexes with both TBP and PPARγ. These findings suggest that TAF7L plays an integral role in adipocyte gene expression by targeting enhancers as a cofactor for PPARγ and promoters as a component of the core transcriptional machinery. |
| first_indexed | 2024-04-12T02:14:48Z |
| format | Article |
| id | doaj.art-a6e7699797ab4c27ae5cdee1ed0000ec |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:14:48Z |
| publishDate | 2013-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a6e7699797ab4c27ae5cdee1ed0000ec2022-12-22T03:52:17ZengeLife Sciences Publications LtdeLife2050-084X2013-01-01210.7554/eLife.00170Dual functions of TAF7L in adipocyte differentiationHaiying Zhou0Tommy Kaplan1Yan Li2Ivan Grubisic3Zhengjian Zhang4P Jeremy Wang5Michael B Eisen6Robert Tjian7Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States; Li Ka Shing Center For Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, California Institute of Quantitative Biosciences, University of California, Berkeley, United States; School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, IsraelJanelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesLi Ka Shing Center For Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United States; UC Berkeley-UCSF Graduate Program in Bioengineering, Department of Molecular and Cell Biology, California Institute of Quantitative Biosciences, University of California, Berkeley, Berkeley, United StatesJanelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesDepartment of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, United StatesDepartment of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States; Department of Molecular and Cell Biology, California Institute of Quantitative Biosciences, University of California, Berkeley, United StatesDepartment of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States; Li Ka Shing Center For Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesThe diverse transcriptional mechanisms governing cellular differentiation and development of mammalian tissue remains poorly understood. Here we report that TAF7L, a paralogue of TFIID subunit TAF7, is enriched in adipocytes and white fat tissue (WAT) in mouse. Depletion of TAF7L reduced adipocyte-specific gene expression, compromised adipocyte differentiation, and WAT development as well. Ectopic expression of TAF7L in myoblasts reprograms these muscle precursors into adipocytes upon induction. Genome-wide mRNA-seq expression profiling and ChIP-seq binding studies confirmed that TAF7L is required for activating adipocyte-specific genes via a dual mechanism wherein it interacts with PPARγ at enhancers and TBP/Pol II at core promoters. In vitro binding studies confirmed that TAF7L forms complexes with both TBP and PPARγ. These findings suggest that TAF7L plays an integral role in adipocyte gene expression by targeting enhancers as a cofactor for PPARγ and promoters as a component of the core transcriptional machinery.https://elifesciences.org/articles/00170ChIP-seqRNA-seqadipogenesisC3H10T½TAF7Ldifferentiation |
| spellingShingle | Haiying Zhou Tommy Kaplan Yan Li Ivan Grubisic Zhengjian Zhang P Jeremy Wang Michael B Eisen Robert Tjian Dual functions of TAF7L in adipocyte differentiation eLife ChIP-seq RNA-seq adipogenesis C3H10T½ TAF7L differentiation |
| title | Dual functions of TAF7L in adipocyte differentiation |
| title_full | Dual functions of TAF7L in adipocyte differentiation |
| title_fullStr | Dual functions of TAF7L in adipocyte differentiation |
| title_full_unstemmed | Dual functions of TAF7L in adipocyte differentiation |
| title_short | Dual functions of TAF7L in adipocyte differentiation |
| title_sort | dual functions of taf7l in adipocyte differentiation |
| topic | ChIP-seq RNA-seq adipogenesis C3H10T½ TAF7L differentiation |
| url | https://elifesciences.org/articles/00170 |
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