The skin sterols of normal and triparanol-treated rats*
The skin sterols of normal rats and of rats treated with the drug triparanol have been analyzed by means of chromatography on silicic acid followed by gas-liquid chromatography of the components of the peaks obtained from the silicic acid chromatogram. These two procedures complement each other in l...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1963-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520403438 |
| _version_ | 1818728855270064128 |
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| author | R.B. Clayton Albert N. Nelson Ivan D. Frantz, Jr. |
| author_facet | R.B. Clayton Albert N. Nelson Ivan D. Frantz, Jr. |
| author_sort | R.B. Clayton |
| collection | DOAJ |
| description | The skin sterols of normal rats and of rats treated with the drug triparanol have been analyzed by means of chromatography on silicic acid followed by gas-liquid chromatography of the components of the peaks obtained from the silicic acid chromatogram. These two procedures complement each other in leading to complete separations of pairs of sterols that are poorly separated in either system alone. The results are shown to indicate the presence in rat skin of several previously undetected compounds for which molecular structures are proposed on the basis of the retention factor method. Evidence is presented for at least two effects of the drug triparanol, (1) in causing accumulation of the Δ24-analogues of all the intermediates in cholesterol biosyntheis that normally occur in the 24,25-dihydro form, and (2) in causing marked alterations in the proportions of Δ7- to Δ8-isomers of these intermediates. The significance of these findings is discussed in the light of current views of the pathway of cholesterol biosynthesis. |
| first_indexed | 2024-12-17T22:36:37Z |
| format | Article |
| id | doaj.art-a6e7eac91de24bf088c17bb22b1a092b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:36:37Z |
| publishDate | 1963-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a6e7eac91de24bf088c17bb22b1a092b2022-12-21T21:30:04ZengElsevierJournal of Lipid Research0022-22751963-04-0142166178The skin sterols of normal and triparanol-treated rats*R.B. Clayton0Albert N. Nelson1Ivan D. Frantz, Jr.2Conant Chemical Laboratory, Harvard University, Cambridge 38, Massachusetts; Departments of Medicine and Physiological Chemistry, University of Minnesota Medical School, Minneapolis 14, MinnesotaConant Chemical Laboratory, Harvard University, Cambridge 38, Massachusetts; Departments of Medicine and Physiological Chemistry, University of Minnesota Medical School, Minneapolis 14, MinnesotaConant Chemical Laboratory, Harvard University, Cambridge 38, Massachusetts; Departments of Medicine and Physiological Chemistry, University of Minnesota Medical School, Minneapolis 14, MinnesotaThe skin sterols of normal rats and of rats treated with the drug triparanol have been analyzed by means of chromatography on silicic acid followed by gas-liquid chromatography of the components of the peaks obtained from the silicic acid chromatogram. These two procedures complement each other in leading to complete separations of pairs of sterols that are poorly separated in either system alone. The results are shown to indicate the presence in rat skin of several previously undetected compounds for which molecular structures are proposed on the basis of the retention factor method. Evidence is presented for at least two effects of the drug triparanol, (1) in causing accumulation of the Δ24-analogues of all the intermediates in cholesterol biosyntheis that normally occur in the 24,25-dihydro form, and (2) in causing marked alterations in the proportions of Δ7- to Δ8-isomers of these intermediates. The significance of these findings is discussed in the light of current views of the pathway of cholesterol biosynthesis.http://www.sciencedirect.com/science/article/pii/S0022227520403438 |
| spellingShingle | R.B. Clayton Albert N. Nelson Ivan D. Frantz, Jr. The skin sterols of normal and triparanol-treated rats* Journal of Lipid Research |
| title | The skin sterols of normal and triparanol-treated rats* |
| title_full | The skin sterols of normal and triparanol-treated rats* |
| title_fullStr | The skin sterols of normal and triparanol-treated rats* |
| title_full_unstemmed | The skin sterols of normal and triparanol-treated rats* |
| title_short | The skin sterols of normal and triparanol-treated rats* |
| title_sort | skin sterols of normal and triparanol treated rats |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520403438 |
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