NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i>
The brown planthopper <i>Nilaparvata lugens</i> is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing <i>N. lugens<...
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MDPI AG
2020-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/9/642 |
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| author | Lu-Yao Peng Zhen-Wei Dai Rui-Rui Yang Zhen Zhu Wei Wang Xiang Zhou Yan-Yuan Bao |
| author_facet | Lu-Yao Peng Zhen-Wei Dai Rui-Rui Yang Zhen Zhu Wei Wang Xiang Zhou Yan-Yuan Bao |
| author_sort | Lu-Yao Peng |
| collection | DOAJ |
| description | The brown planthopper <i>Nilaparvata lugens</i> is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing <i>N. lugens</i> populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. <i>Nilaparvata lugens</i> is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, <i>NADPH oxidase 5</i> (<i>Nox5</i>), is essential for molting and oviposition in a Hemipteran insect <i>Nilaparvata lugens</i>. Knockdown of <i>Nox5</i> transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. <i>Nox5</i> knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that <i>Nox5</i> is a key enzyme for regulating molting and oviposition in this insect species. |
| first_indexed | 2024-03-10T16:13:50Z |
| format | Article |
| id | doaj.art-a6eff43ed791409f83bb921212f776cb |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T16:13:50Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-a6eff43ed791409f83bb921212f776cb2023-11-20T14:14:38ZengMDPI AGInsects2075-44502020-09-0111964210.3390/insects11090642NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i>Lu-Yao Peng0Zhen-Wei Dai1Rui-Rui Yang2Zhen Zhu3Wei Wang4Xiang Zhou5Yan-Yuan Bao6State Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaThe brown planthopper <i>Nilaparvata lugens</i> is a typical monophagous insect herbivore that feeds exclusively on rice sap. This insect pest causes serious damage to rice crops throughout East Asian countries. Chemical control remains the first choice for managing <i>N. lugens</i> populations; however, the use of insecticides has given rise to planthopper resurgence and additional environmental risks. <i>Nilaparvata lugens</i> is a model insect of Hemiptera because its whole genome sequence has been elucidated and is susceptible to RNA interference. In this study, our findings revealed that a superoxide-generating gene, <i>NADPH oxidase 5</i> (<i>Nox5</i>), is essential for molting and oviposition in a Hemipteran insect <i>Nilaparvata lugens</i>. Knockdown of <i>Nox5</i> transcript levels by RNA interference in 2nd–5th-instar nymphs results in significantly lethal deficits in the molting transitions from nymph–nymph and nymph–adult. <i>Nox5</i> knockdown leads to a reduction of hydrogen peroxide in female ovaries and failure of oviposition from the insect ovipositor into the rice leaf sheath. Here, we provide in vivo evidence demonstrating that <i>Nox5</i> is a key enzyme for regulating molting and oviposition in this insect species.https://www.mdpi.com/2075-4450/11/9/642<i>Nilaparvata lugens</i><i>Nox5</i>moltingovipositionRNAi |
| spellingShingle | Lu-Yao Peng Zhen-Wei Dai Rui-Rui Yang Zhen Zhu Wei Wang Xiang Zhou Yan-Yuan Bao NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> Insects <i>Nilaparvata lugens</i> <i>Nox5</i> molting oviposition RNAi |
| title | NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> |
| title_full | NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> |
| title_fullStr | NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> |
| title_full_unstemmed | NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> |
| title_short | NADPH Oxidase 5 Is Essential for Molting and Oviposition in a Rice Planthopper <i>Nilaparvata lugens</i> |
| title_sort | nadph oxidase 5 is essential for molting and oviposition in a rice planthopper i nilaparvata lugens i |
| topic | <i>Nilaparvata lugens</i> <i>Nox5</i> molting oviposition RNAi |
| url | https://www.mdpi.com/2075-4450/11/9/642 |
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