| Summary: | In this study, a new series of <i>N</i>-alkyl-3,6-dibromocarbazole and <i>N</i>-alkyl-5-bromoindole derivatives have been synthesized and evaluated in vitro as anti-cancer and anti-migration agents. Cytotoxic and anti-migratory effects of these compounds were evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines and an insight on the structure-activity relationship was developed. Preliminary investigations of their anti-cancer activity demonstrated that several compounds have moderate antiproliferative effects on cancer cell lines with GI<sub>50</sub> values in the range of 4.7−32.2 µM. Moreover, carbazole derivatives <b>10</b>, <b>14</b>, <b>15</b>, <b>23</b>, and <b>24</b> inhibit migration activity of metastatic cell line MDA-MB-231 in the range of 18−20%. The effect of compounds <b>10</b>, <b>14</b>, and <b>15</b> in extension of invadopodia and filopodia was evaluated by fluorescence microscopy and results demonstrated a reduction in actin-based cell extensions by compounds <b>10</b> and <b>15</b>.
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