The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates
In viticulture, grafting is used to propagate Phylloxera-susceptible European grapevines, thereby using resistant American rootstocks. Although scion–rootstock reciprocal signaling is essential for the formation of a proper vascular union and for coordinated growth, our knowledge of graft partner in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/6/349 |
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| author | Sara Tedesco Alexander Erban Saurabh Gupta Joachim Kopka Pedro Fevereiro Friedrich Kragler Ana Pina |
| author_facet | Sara Tedesco Alexander Erban Saurabh Gupta Joachim Kopka Pedro Fevereiro Friedrich Kragler Ana Pina |
| author_sort | Sara Tedesco |
| collection | DOAJ |
| description | In viticulture, grafting is used to propagate Phylloxera-susceptible European grapevines, thereby using resistant American rootstocks. Although scion–rootstock reciprocal signaling is essential for the formation of a proper vascular union and for coordinated growth, our knowledge of graft partner interactions is very limited. In order to elucidate the scale and the content of scion–rootstock metabolic interactions, we profiled the metabolome of eleven graft combination in leaves, stems, and phloem exudate from both above and below the graft union 5–6 months after grafting. We compared the metabolome of scions vs. rootstocks of homografts vs. heterografts and investigated the reciprocal effect of the rootstock on the scion metabolome. This approach revealed that (1) grafting has a minor impact on the metabolome of grafted grapevines when tissues and genotypes were compared, (2) heterografting affects rootstocks more than scions, (3) the presence of a heterologous grafting partner increases defense-related compounds in both scion and rootstocks in shorter and longer distances from the graft, and (4) leaves were revealed as the best tissue to search for grafting-related metabolic markers. These results will provide a valuable metabolomics resource for scion–rootstock interaction studies and will facilitate future efforts on the identification of metabolic markers for important agronomic traits in grafted grapevines. |
| first_indexed | 2024-03-10T10:53:53Z |
| format | Article |
| id | doaj.art-a6fa5f7835b64b6f9ffe22807f47454d |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T10:53:53Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-a6fa5f7835b64b6f9ffe22807f47454d2023-11-21T22:03:58ZengMDPI AGMetabolites2218-19892021-05-0111634910.3390/metabo11060349The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem ExudatesSara Tedesco0Alexander Erban1Saurabh Gupta2Joachim Kopka3Pedro Fevereiro4Friedrich Kragler5Ana Pina6Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-It Unit), Avenida da República, Estação Agronómica Nacional, 2780-157 Oeiras, PortugalApplied Metabolome Analysis Laboratory, Max Planck Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, GermanyDepartment 2, Max Planck Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, GermanyApplied Metabolome Analysis Laboratory, Max Planck Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, GermanyPlant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier (Green-It Unit), Avenida da República, Estação Agronómica Nacional, 2780-157 Oeiras, PortugalDepartment 2, Max Planck Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam-Golm, GermanyUnidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avenida Montañana 930, 50059 Zaragoza, SpainIn viticulture, grafting is used to propagate Phylloxera-susceptible European grapevines, thereby using resistant American rootstocks. Although scion–rootstock reciprocal signaling is essential for the formation of a proper vascular union and for coordinated growth, our knowledge of graft partner interactions is very limited. In order to elucidate the scale and the content of scion–rootstock metabolic interactions, we profiled the metabolome of eleven graft combination in leaves, stems, and phloem exudate from both above and below the graft union 5–6 months after grafting. We compared the metabolome of scions vs. rootstocks of homografts vs. heterografts and investigated the reciprocal effect of the rootstock on the scion metabolome. This approach revealed that (1) grafting has a minor impact on the metabolome of grafted grapevines when tissues and genotypes were compared, (2) heterografting affects rootstocks more than scions, (3) the presence of a heterologous grafting partner increases defense-related compounds in both scion and rootstocks in shorter and longer distances from the graft, and (4) leaves were revealed as the best tissue to search for grafting-related metabolic markers. These results will provide a valuable metabolomics resource for scion–rootstock interaction studies and will facilitate future efforts on the identification of metabolic markers for important agronomic traits in grafted grapevines.https://www.mdpi.com/2218-1989/11/6/349graftinggrapevinemetabolic profilesrootstocksphloem exudatescion–rootstock interactions |
| spellingShingle | Sara Tedesco Alexander Erban Saurabh Gupta Joachim Kopka Pedro Fevereiro Friedrich Kragler Ana Pina The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates Metabolites grafting grapevine metabolic profiles rootstocks phloem exudate scion–rootstock interactions |
| title | The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates |
| title_full | The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates |
| title_fullStr | The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates |
| title_full_unstemmed | The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates |
| title_short | The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates |
| title_sort | impact of metabolic scion rootstock interactions in different grapevine tissues and phloem exudates |
| topic | grafting grapevine metabolic profiles rootstocks phloem exudate scion–rootstock interactions |
| url | https://www.mdpi.com/2218-1989/11/6/349 |
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