Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City
【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the path...
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Guangdong Academy of Agricultural Sciences
2022-12-01
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| Series: | Guangdong nongye kexue |
| Subjects: | |
| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202212011 |
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| author | Yiping CUI AIitian PENG Xiaobing SONG Guanjie FENG Feng HUANG Xia CHEN Jinfeng LING Baoping CHENG |
| author_facet | Yiping CUI AIitian PENG Xiaobing SONG Guanjie FENG Feng HUANG Xia CHEN Jinfeng LING Baoping CHENG |
| author_sort | Yiping CUI |
| collection | DOAJ |
| description | 【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-1, ST4-2, and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification.【Result】ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2-2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4-1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.【Conclusion】Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C.siamense, C.cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C. fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants. |
| first_indexed | 2024-03-13T05:40:54Z |
| format | Article |
| id | doaj.art-a6fa831185d24f69b5216e7a7c67971d |
| institution | Directory Open Access Journal |
| issn | 1004-874X |
| language | English |
| last_indexed | 2024-03-13T05:40:54Z |
| publishDate | 2022-12-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj.art-a6fa831185d24f69b5216e7a7c67971d2023-06-14T00:54:55ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2022-12-0149129910910.16768/j.issn.1004-874X.2022.12.011202212011Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou CityYiping CUI0AIitian PENG1Xiaobing SONG2Guanjie FENG3Feng HUANG4Xia CHEN5Jinfeng LING6Baoping CHENG7Institute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaShatian Lemon Agricultural Products Cooperative of Baiyun District, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, ChinaInstitute of Plant Protection, Guangdong Academy of Agricultural Sciences / Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-1, ST4-2, and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification.【Result】ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2-2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4-1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.【Conclusion】Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C.siamense, C.cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C. fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202212011shatian lemonguangzhou cityanthracnosecolletotrichum fructicolacolletotrichum cliviicola colletotrichum siamense |
| spellingShingle | Yiping CUI AIitian PENG Xiaobing SONG Guanjie FENG Feng HUANG Xia CHEN Jinfeng LING Baoping CHENG Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City Guangdong nongye kexue shatian lemon guangzhou city anthracnose colletotrichum fructicola colletotrichum cliviicola colletotrichum siamense |
| title | Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City |
| title_full | Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City |
| title_fullStr | Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City |
| title_full_unstemmed | Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City |
| title_short | Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City |
| title_sort | molecular identification of pathogens causing anthracnose on shatian lemon in guangzhou city |
| topic | shatian lemon guangzhou city anthracnose colletotrichum fructicola colletotrichum cliviicola colletotrichum siamense |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202212011 |
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