IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS
Abstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immun...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/122 |
| _version_ | 1797198178075803648 |
|---|---|
| author | E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh |
| author_facet | E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh |
| author_sort | E. V. Kurganova |
| collection | DOAJ |
| description | Abstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immunosuppressive agents (vitamin D3 and dexamethasone, model 2). It was shown, that the Treg, obtained in model 1, are characterized by decreased anti-CD3-induced proliferative response and lack of suppressive activity. Treg, obtained in presence of vitamin D3 and dexamethasone, proved to be profoundly anergic. Such a state may be partially abolished by exogenic IL-2. These cells are also characterized by suppressive activity mediated by both cell-to-cell contacts and soluble factors, such as IL-10. Hence, the regulatory T cells generated in model 2, can be referred as inducible Treg (Tr1). Nevertheless, increased ratio of cells expressing CD4+FOXP3+ among Treg obtained by model 2, may also suggest an ability to generate natural regulatory cells. (Med. Immunol., 2008, vol. 10, n 2-3, pp 173-180). |
| first_indexed | 2024-03-08T05:51:51Z |
| format | Article |
| id | doaj.art-a6ff34451673448cbc832128ac821826 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:55:43Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-a6ff34451673448cbc832128ac8218262024-04-22T13:07:33ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01102-317318010.15789/1563-0625-2008-2-3-173-180119IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLSE. V. Kurganova0E. Ya. Shevela1M. A. Tihonova2A. A. Ostanin3E. R. Chernykh4ГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскAbstract. The aim of present study was to evaluate efficacy of generation and characterization of regulatory T cells (Treg). The Treg were derived from peripheral mononuclear cells of healthy donors, using activation with anti-CD3mAb and IL-2, either in absence (model 1), or in the presence of immunosuppressive agents (vitamin D3 and dexamethasone, model 2). It was shown, that the Treg, obtained in model 1, are characterized by decreased anti-CD3-induced proliferative response and lack of suppressive activity. Treg, obtained in presence of vitamin D3 and dexamethasone, proved to be profoundly anergic. Such a state may be partially abolished by exogenic IL-2. These cells are also characterized by suppressive activity mediated by both cell-to-cell contacts and soluble factors, such as IL-10. Hence, the regulatory T cells generated in model 2, can be referred as inducible Treg (Tr1). Nevertheless, increased ratio of cells expressing CD4+FOXP3+ among Treg obtained by model 2, may also suggest an ability to generate natural regulatory cells. (Med. Immunol., 2008, vol. 10, n 2-3, pp 173-180).https://www.mimmun.ru/mimmun/article/view/122тregcd4cd25cd4foxp3il-10vitamin d3dexamethasone |
| spellingShingle | E. V. Kurganova E. Ya. Shevela M. A. Tihonova A. A. Ostanin E. R. Chernykh IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS Медицинская иммунология тreg cd4cd25 cd4foxp3 il-10 vitamin d3 dexamethasone |
| title | IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS |
| title_full | IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS |
| title_fullStr | IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS |
| title_full_unstemmed | IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS |
| title_short | IN VITRO GENERATION AND CHARACTERIZATION OF HUMAN REGULATORY T-CELLS |
| title_sort | in vitro generation and characterization of human regulatory t cells |
| topic | тreg cd4cd25 cd4foxp3 il-10 vitamin d3 dexamethasone |
| url | https://www.mimmun.ru/mimmun/article/view/122 |
| work_keys_str_mv | AT evkurganova invitrogenerationandcharacterizationofhumanregulatorytcells AT eyashevela invitrogenerationandcharacterizationofhumanregulatorytcells AT matihonova invitrogenerationandcharacterizationofhumanregulatorytcells AT aaostanin invitrogenerationandcharacterizationofhumanregulatorytcells AT erchernykh invitrogenerationandcharacterizationofhumanregulatorytcells |