Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells
<p>Abstract</p> <p>Background</p> <p>Cell-based therapies show promise in repairing cardiac tissue and improving contractile performance following a myocardial infarction. Despite this, ischemia-induced death of transplanted cells remains a major hurdle to the efficacy...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2012-06-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | http://www.cellregenerationjournal.com/content/1/1/3 |
| _version_ | 1818758988203819008 |
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| author | Hughey Curtis C Alfaro Maria P Belke Darrell D Rottman Jeffery N Young Pampee P Wasserman David H Shearer Jane |
| author_facet | Hughey Curtis C Alfaro Maria P Belke Darrell D Rottman Jeffery N Young Pampee P Wasserman David H Shearer Jane |
| author_sort | Hughey Curtis C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cell-based therapies show promise in repairing cardiac tissue and improving contractile performance following a myocardial infarction. Despite this, ischemia-induced death of transplanted cells remains a major hurdle to the efficacy of treatment. ‘<it>Superhealer</it>’ MRL/MpJ mesenchymal stem cells (MRL-MSCs) have been reported to exhibit increased engraftment resulting in reduced infarct size and enhanced contractile function. This study determines whether intrinsic differences in mitochondrial oxidative phosphorylation (OXPHOS) assist in explaining the enhanced cellular survival and engraftment of MRL-MSCs.</p> <p>Findings</p> <p>Compared to wild type MSCs (WT-MSCs), mitochondria from intact MRL-MSCs exhibited an increase in routine respiration and maximal electron transport capacity by 2.0- and 3.5-fold, respectively. When routine oxygen utilization is expressed as a portion of maximal cellular oxygen flux, the MRL-MSCs have a greater spare respiratory capcity. Additionally, glutamate/malate succinate-supported oxygen consumption in permeabilized cells was elevated approximately 1.25- and 1.4-fold in the MRL-MSCs, respectively.</p> <p>Conclusion</p> <p>The results from intact and permeabilized MSCs indicate MRL-MSCs exhibit a greater reliance on and capacity for aerobic metabolism. The greater capacity for oxidative metabolism may provide a protective effect by increasing ATP synthesis per unit substrate and prevent glycolysis-mediated acidosis and subsequent cell death upon transplantation into the glucose-and oxygen-deprived environment of the infarcted heart.</p> |
| first_indexed | 2024-12-18T06:35:34Z |
| format | Article |
| id | doaj.art-a7002edca22840d294d362088e6627be |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-12-18T06:35:34Z |
| publishDate | 2012-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-a7002edca22840d294d362088e6627be2022-12-21T21:17:48ZengSpringerOpenCell Regeneration2045-97692012-06-0111310.1186/2045-9769-1-3Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cellsHughey Curtis CAlfaro Maria PBelke Darrell DRottman Jeffery NYoung Pampee PWasserman David HShearer Jane<p>Abstract</p> <p>Background</p> <p>Cell-based therapies show promise in repairing cardiac tissue and improving contractile performance following a myocardial infarction. Despite this, ischemia-induced death of transplanted cells remains a major hurdle to the efficacy of treatment. ‘<it>Superhealer</it>’ MRL/MpJ mesenchymal stem cells (MRL-MSCs) have been reported to exhibit increased engraftment resulting in reduced infarct size and enhanced contractile function. This study determines whether intrinsic differences in mitochondrial oxidative phosphorylation (OXPHOS) assist in explaining the enhanced cellular survival and engraftment of MRL-MSCs.</p> <p>Findings</p> <p>Compared to wild type MSCs (WT-MSCs), mitochondria from intact MRL-MSCs exhibited an increase in routine respiration and maximal electron transport capacity by 2.0- and 3.5-fold, respectively. When routine oxygen utilization is expressed as a portion of maximal cellular oxygen flux, the MRL-MSCs have a greater spare respiratory capcity. Additionally, glutamate/malate succinate-supported oxygen consumption in permeabilized cells was elevated approximately 1.25- and 1.4-fold in the MRL-MSCs, respectively.</p> <p>Conclusion</p> <p>The results from intact and permeabilized MSCs indicate MRL-MSCs exhibit a greater reliance on and capacity for aerobic metabolism. The greater capacity for oxidative metabolism may provide a protective effect by increasing ATP synthesis per unit substrate and prevent glycolysis-mediated acidosis and subsequent cell death upon transplantation into the glucose-and oxygen-deprived environment of the infarcted heart.</p>http://www.cellregenerationjournal.com/content/1/1/3EnergeticsMitochondriaOxidative phosphorylationStem cells |
| spellingShingle | Hughey Curtis C Alfaro Maria P Belke Darrell D Rottman Jeffery N Young Pampee P Wasserman David H Shearer Jane Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells Cell Regeneration Energetics Mitochondria Oxidative phosphorylation Stem cells |
| title | Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells |
| title_full | Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells |
| title_fullStr | Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells |
| title_full_unstemmed | Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells |
| title_short | Increased oxygen consumption and OXPHOS potential in superhealer mesenchymal stem cells |
| title_sort | increased oxygen consumption and oxphos potential in superhealer mesenchymal stem cells |
| topic | Energetics Mitochondria Oxidative phosphorylation Stem cells |
| url | http://www.cellregenerationjournal.com/content/1/1/3 |
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