Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer
We developed a reliable simple analysis of adenosine, creatinine and other nucleosides (cytidine and guanosine) with single measurement based on surface-enhanced Raman spectroscopy (SERS) using DSP-AuNPs with Cu2+ ions probe and applied it to achieve noninvasive screening of lung cancer. The gold na...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-12-01
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| Series: | Engineered Regeneration |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266613812200055X |
| _version_ | 1797984334970355712 |
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| author | Xueqian Ren Liping Huang Chengde Wang Yuancai Ge Kaili Zhang Danfeng Jiang Xiaohu Liu Qingwen Zhang Yi Wang |
| author_facet | Xueqian Ren Liping Huang Chengde Wang Yuancai Ge Kaili Zhang Danfeng Jiang Xiaohu Liu Qingwen Zhang Yi Wang |
| author_sort | Xueqian Ren |
| collection | DOAJ |
| description | We developed a reliable simple analysis of adenosine, creatinine and other nucleosides (cytidine and guanosine) with single measurement based on surface-enhanced Raman spectroscopy (SERS) using DSP-AuNPs with Cu2+ ions probe and applied it to achieve noninvasive screening of lung cancer. The gold nanoparticles (AuNPs) were modified with 3,3′-dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP), which could react with above mentioned molecules followed with the aggregation of AuNPs at the presence of Cu2+, thus form the plasmonic hot spots to dramatically increase their fingerprint Raman signal. The probe of DSP-AuNPs was applied for the detail analysis of urinary adenosine of healthy people and lung cancer patients. The SERS measurement indicated a higher concentration of urinary adenosine for lung cancer patients than that of healthy people, which was consistent with the results measured by high-performance liquid chromatography (HPLC). In combination of Raman spectroscopy with the orthogonal partial least squares discriminant analysis (OPLS-DA) model, we are able to discriminate the lung cancer patients from healthy people with the urinary test. The results indicated that besides of adenosine, other metabolites including uric acid, guanine and creatinine may also be the potential tumor markers in urine for the noninvasive lung cancer diagnosis. Such a method paves a way for the noninvasive cancer screening and it can be further modified for the detection of other molecules on medical diagnosis. |
| first_indexed | 2024-04-11T07:00:21Z |
| format | Article |
| id | doaj.art-a7048b9d0012402ca92524a25686553d |
| institution | Directory Open Access Journal |
| issn | 2666-1381 |
| language | English |
| last_indexed | 2024-04-11T07:00:21Z |
| publishDate | 2022-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Engineered Regeneration |
| spelling | doaj.art-a7048b9d0012402ca92524a25686553d2022-12-22T04:38:48ZengKeAi Communications Co., Ltd.Engineered Regeneration2666-13812022-12-0134387396Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancerXueqian Ren0Liping Huang1Chengde Wang2Yuancai Ge3Kaili Zhang4Danfeng Jiang5Xiaohu Liu6Qingwen Zhang7Yi Wang8School of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, ChinaSchool of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, ChinaThe First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325001, ChinaSchool of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, ChinaSchool of Opto-Electronic Engineering, Changchun University of Science and Technology, Changchun 130022, ChinaSchool of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, ChinaSchool of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, ChinaSchool of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China; Corresponding authors.School of Biomedical Engineering, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325001, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China; School of Opto-Electronic Engineering, Changchun University of Science and Technology, Changchun 130022, China; Corresponding authors.We developed a reliable simple analysis of adenosine, creatinine and other nucleosides (cytidine and guanosine) with single measurement based on surface-enhanced Raman spectroscopy (SERS) using DSP-AuNPs with Cu2+ ions probe and applied it to achieve noninvasive screening of lung cancer. The gold nanoparticles (AuNPs) were modified with 3,3′-dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP), which could react with above mentioned molecules followed with the aggregation of AuNPs at the presence of Cu2+, thus form the plasmonic hot spots to dramatically increase their fingerprint Raman signal. The probe of DSP-AuNPs was applied for the detail analysis of urinary adenosine of healthy people and lung cancer patients. The SERS measurement indicated a higher concentration of urinary adenosine for lung cancer patients than that of healthy people, which was consistent with the results measured by high-performance liquid chromatography (HPLC). In combination of Raman spectroscopy with the orthogonal partial least squares discriminant analysis (OPLS-DA) model, we are able to discriminate the lung cancer patients from healthy people with the urinary test. The results indicated that besides of adenosine, other metabolites including uric acid, guanine and creatinine may also be the potential tumor markers in urine for the noninvasive lung cancer diagnosis. Such a method paves a way for the noninvasive cancer screening and it can be further modified for the detection of other molecules on medical diagnosis.http://www.sciencedirect.com/science/article/pii/S266613812200055XAdenosineSurface-enhanced Raman scatteringGold nanoparticlesOPLS-DALung cancerNoninvasive diagnosis |
| spellingShingle | Xueqian Ren Liping Huang Chengde Wang Yuancai Ge Kaili Zhang Danfeng Jiang Xiaohu Liu Qingwen Zhang Yi Wang Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer Engineered Regeneration Adenosine Surface-enhanced Raman scattering Gold nanoparticles OPLS-DA Lung cancer Noninvasive diagnosis |
| title | Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer |
| title_full | Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer |
| title_fullStr | Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer |
| title_full_unstemmed | Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer |
| title_short | Urinary analysis based on surface-enhanced Raman scattering for the noninvasive screening of lung cancer |
| title_sort | urinary analysis based on surface enhanced raman scattering for the noninvasive screening of lung cancer |
| topic | Adenosine Surface-enhanced Raman scattering Gold nanoparticles OPLS-DA Lung cancer Noninvasive diagnosis |
| url | http://www.sciencedirect.com/science/article/pii/S266613812200055X |
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