A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China
The aim of this study was to elucidate the prevalence of <i>bla</i><sub>CTX-M-27</sub>-producing <i>Escherichia coli</i> and transmission mechanisms of <i>bla</i><sub>CTX-M-27</sub> from swine farms in China. A total of 333 <i>E. coli...
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/4/387 |
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| author | Yan Zhang Yin-Huan Sun Jiang-Yang Wang Man-Xia Chang Qiu-Yun Zhao Hong-Xia Jiang |
| author_facet | Yan Zhang Yin-Huan Sun Jiang-Yang Wang Man-Xia Chang Qiu-Yun Zhao Hong-Xia Jiang |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | The aim of this study was to elucidate the prevalence of <i>bla</i><sub>CTX-M-27</sub>-producing <i>Escherichia coli</i> and transmission mechanisms of <i>bla</i><sub>CTX-M-27</sub> from swine farms in China. A total of 333 <i>E. coli</i> isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive <i>E. coli</i> were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried <i>bla</i><sub>CTX-M-27</sub>, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried <i>bla</i><sub>CTX-M-27</sub> genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The <i>bla</i><sub>CTX-M-27</sub> genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with <i>bla</i><sub>CTX-M-27</sub>-plasmids indicated that the <i>tra-trb</i> region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing <i>bla</i><sub>CTX-M-27</sub> in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the <i>bla</i><sub>CTX-M-27</sub> gene in <i>E. coli</i>. This study indicated that the predominant vehicle for <i>bla</i><sub>CTX-M-27</sub> transmission has diversified over time and that control strategies to limit <i>bla</i><sub>CTX-M-27</sub> transmission in farm animals are necessary. |
| first_indexed | 2024-03-10T12:36:56Z |
| format | Article |
| id | doaj.art-a707151c3f50492a9c451f85942c4886 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:36:56Z |
| publishDate | 2021-04-01 |
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| series | Antibiotics |
| spelling | doaj.art-a707151c3f50492a9c451f85942c48862023-11-21T14:12:37ZengMDPI AGAntibiotics2079-63822021-04-0110438710.3390/antibiotics10040387A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in ChinaYan Zhang0Yin-Huan Sun1Jiang-Yang Wang2Man-Xia Chang3Qiu-Yun Zhao4Hong-Xia Jiang5Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaThe aim of this study was to elucidate the prevalence of <i>bla</i><sub>CTX-M-27</sub>-producing <i>Escherichia coli</i> and transmission mechanisms of <i>bla</i><sub>CTX-M-27</sub> from swine farms in China. A total of 333 <i>E. coli</i> isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive <i>E. coli</i> were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried <i>bla</i><sub>CTX-M-27</sub>, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried <i>bla</i><sub>CTX-M-27</sub> genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The <i>bla</i><sub>CTX-M-27</sub> genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with <i>bla</i><sub>CTX-M-27</sub>-plasmids indicated that the <i>tra-trb</i> region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing <i>bla</i><sub>CTX-M-27</sub> in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the <i>bla</i><sub>CTX-M-27</sub> gene in <i>E. coli</i>. This study indicated that the predominant vehicle for <i>bla</i><sub>CTX-M-27</sub> transmission has diversified over time and that control strategies to limit <i>bla</i><sub>CTX-M-27</sub> transmission in farm animals are necessary.https://www.mdpi.com/2079-6382/10/4/387<i>bla</i><sub>CTX-M-27</sub>Tn<i>2</i>plasmid<i>Escherichia coli</i>ST10 |
| spellingShingle | Yan Zhang Yin-Huan Sun Jiang-Yang Wang Man-Xia Chang Qiu-Yun Zhao Hong-Xia Jiang A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China Antibiotics <i>bla</i><sub>CTX-M-27</sub> Tn<i>2</i> plasmid <i>Escherichia coli</i> ST10 |
| title | A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China |
| title_full | A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China |
| title_fullStr | A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China |
| title_full_unstemmed | A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China |
| title_short | A Novel Structure Harboring <i>bla</i><sub>CTX-M-27</sub> on IncF Plasmids in <i>Escherichia coli</i> Isolated from Swine in China |
| title_sort | novel structure harboring i bla i sub ctx m 27 sub on incf plasmids in i escherichia coli i isolated from swine in china |
| topic | <i>bla</i><sub>CTX-M-27</sub> Tn<i>2</i> plasmid <i>Escherichia coli</i> ST10 |
| url | https://www.mdpi.com/2079-6382/10/4/387 |
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