Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway
Aim: This study aimed to investigate the effect of norcantharidin (NCTD) on human mesangial cells (HMCs) apoptosis in vitro and further examine its molecular mechanism. Methods: HMCs were divided into 5 groups: control group, 25% fetal bovine serum (FBS)-treated group, and NCTD groups (NCTD [2.5, 5...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Karger Publishers
2019-10-01
|
| Series: | Kidney & Blood Pressure Research |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/502524 |
| _version_ | 1818268926574854144 |
|---|---|
| author | Kun Ye Qiao-Yu Wei Teng-Xiang Long Hong-Guang He Yun-Feng Huang Li-Jia Xiong Jiao Lan Yi-Yun Huang Zhi-Feng Gong Xiao-Mei Peng Qiu-Xia Wu |
| author_facet | Kun Ye Qiao-Yu Wei Teng-Xiang Long Hong-Guang He Yun-Feng Huang Li-Jia Xiong Jiao Lan Yi-Yun Huang Zhi-Feng Gong Xiao-Mei Peng Qiu-Xia Wu |
| author_sort | Kun Ye |
| collection | DOAJ |
| description | Aim: This study aimed to investigate the effect of norcantharidin (NCTD) on human mesangial cells (HMCs) apoptosis in vitro and further examine its molecular mechanism. Methods: HMCs were divided into 5 groups: control group, 25% fetal bovine serum (FBS)-treated group, and NCTD groups (NCTD [2.5, 5 and 10 µg/mL] + 25% FBS, respectively). Cell proliferation was determined by MTT assay, while apoptosis was evaluated by Hoechest 33258 staining, the level of cytochrome c, immunohistochemistry, and apoptotic-related proteins/gene expression. Results: Cell viability was inhibited in NCTD-treated HMCs in a dose-dependent manner. The number of apoptotic cells and the content of cytochrome c were significantly increased by NCTD treatment but that of mitochondrial membrane was decreased. Moreover, the expression of bcl-2 and caspase-3 was prompted by NCTD, but the expression of bax, MMP-2, and MMP-9 in 25% FBS-treated HMCs was inhibited. In addition, NCTD markedly unregulated the expression of apoptosis-related gene/protein, including p-Erk1/2, phosphorylated-Jun N-terminal kinase (JNK), p-p38, and p53. Conclusion: NCTD enhances 25% FBS-treated HMC apoptosis in vitro, and this effect may be attributed to the modulation of the ERK, JNK, and p38 mitogen-activated protein kinase signaling pathways. |
| first_indexed | 2024-12-12T20:46:15Z |
| format | Article |
| id | doaj.art-a707eae5b1b242a99ed24e098eb2931a |
| institution | Directory Open Access Journal |
| issn | 1420-4096 1423-0143 |
| language | English |
| last_indexed | 2024-12-12T20:46:15Z |
| publishDate | 2019-10-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Kidney & Blood Pressure Research |
| spelling | doaj.art-a707eae5b1b242a99ed24e098eb2931a2022-12-22T00:12:34ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432019-10-0111310.1159/000502524502524Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling PathwayKun YeQiao-Yu WeiTeng-Xiang LongHong-Guang HeYun-Feng HuangLi-Jia XiongJiao LanYi-Yun HuangZhi-Feng GongXiao-Mei PengQiu-Xia WuAim: This study aimed to investigate the effect of norcantharidin (NCTD) on human mesangial cells (HMCs) apoptosis in vitro and further examine its molecular mechanism. Methods: HMCs were divided into 5 groups: control group, 25% fetal bovine serum (FBS)-treated group, and NCTD groups (NCTD [2.5, 5 and 10 µg/mL] + 25% FBS, respectively). Cell proliferation was determined by MTT assay, while apoptosis was evaluated by Hoechest 33258 staining, the level of cytochrome c, immunohistochemistry, and apoptotic-related proteins/gene expression. Results: Cell viability was inhibited in NCTD-treated HMCs in a dose-dependent manner. The number of apoptotic cells and the content of cytochrome c were significantly increased by NCTD treatment but that of mitochondrial membrane was decreased. Moreover, the expression of bcl-2 and caspase-3 was prompted by NCTD, but the expression of bax, MMP-2, and MMP-9 in 25% FBS-treated HMCs was inhibited. In addition, NCTD markedly unregulated the expression of apoptosis-related gene/protein, including p-Erk1/2, phosphorylated-Jun N-terminal kinase (JNK), p-p38, and p53. Conclusion: NCTD enhances 25% FBS-treated HMC apoptosis in vitro, and this effect may be attributed to the modulation of the ERK, JNK, and p38 mitogen-activated protein kinase signaling pathways.https://www.karger.com/Article/FullText/502524norcantharidinhuman mesangial cellsapoptosismitogen-activated protein kinase pathway |
| spellingShingle | Kun Ye Qiao-Yu Wei Teng-Xiang Long Hong-Guang He Yun-Feng Huang Li-Jia Xiong Jiao Lan Yi-Yun Huang Zhi-Feng Gong Xiao-Mei Peng Qiu-Xia Wu Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway Kidney & Blood Pressure Research norcantharidin human mesangial cells apoptosis mitogen-activated protein kinase pathway |
| title | Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_full | Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_fullStr | Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_full_unstemmed | Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_short | Norcantharidin Enhances High Concentrations of Fetal Bovine Serum-Induced Apoptosis in Human Mesangial Cells by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway |
| title_sort | norcantharidin enhances high concentrations of fetal bovine serum induced apoptosis in human mesangial cells by regulating the mitogen activated protein kinase signaling pathway |
| topic | norcantharidin human mesangial cells apoptosis mitogen-activated protein kinase pathway |
| url | https://www.karger.com/Article/FullText/502524 |
| work_keys_str_mv | AT kunye norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT qiaoyuwei norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT tengxianglong norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT hongguanghe norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT yunfenghuang norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT lijiaxiong norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT jiaolan norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT yiyunhuang norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT zhifenggong norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT xiaomeipeng norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway AT qiuxiawu norcantharidinenhanceshighconcentrationsoffetalbovineseruminducedapoptosisinhumanmesangialcellsbyregulatingthemitogenactivatedproteinkinasesignalingpathway |