Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy
At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Neuroanatomy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2021.732520/full |
| _version_ | 1819025333751382016 |
|---|---|
| author | Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher |
| author_facet | Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher |
| author_sort | Sebastian Britz |
| collection | DOAJ |
| description | At the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments. |
| first_indexed | 2024-12-21T05:09:01Z |
| format | Article |
| id | doaj.art-a70fe88442874f8daf0bd38af6121fae |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-21T05:09:01Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-a70fe88442874f8daf0bd38af6121fae2022-12-21T19:15:07ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292021-11-011510.3389/fnana.2021.732520732520Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron MicroscopySebastian Britz0Sebastian Matthias Markert1Daniel Witvliet2Daniel Witvliet3Anna Maria Steyer4Sarah Tröger5Ben Mulcahy6Ben Mulcahy7Philip Kollmannsberger8Yannick Schwab9Mei Zhen10Mei Zhen11Christian Stigloher12Imaging Core Facility of the Biocenter, Theodor-Boveri-Institute, Julius-Maximilians-University, Würzburg, GermanyImaging Core Facility of the Biocenter, Theodor-Boveri-Institute, Julius-Maximilians-University, Würzburg, GermanyLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaDepartment of Molecular Genetics, Physiology and Institute of Medical Science, University of Toronto, Toronto, ON, CanadaEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, GermanyImaging Core Facility of the Biocenter, Theodor-Boveri-Institute, Julius-Maximilians-University, Würzburg, GermanyLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaDepartment of Molecular Genetics, Physiology and Institute of Medical Science, University of Toronto, Toronto, ON, CanadaCenter for Computational and Theoretical Biology, Julius-Maximilians-University, Würzburg, GermanyEuropean Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, GermanyLunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, CanadaDepartment of Molecular Genetics, Physiology and Institute of Medical Science, University of Toronto, Toronto, ON, CanadaImaging Core Facility of the Biocenter, Theodor-Boveri-Institute, Julius-Maximilians-University, Würzburg, GermanyAt the end of the first larval stage, the nematode Caenorhabditis elegans developing in harsh environmental conditions is able to choose an alternative developmental path called the dauer diapause. Dauer larvae exhibit different physiology and behaviors from non-dauer larvae. Using focused ion beam-scanning electron microscopy (FIB-SEM), we volumetrically reconstructed the anterior sensory apparatus of C. elegans dauer larvae with unprecedented precision. We provide a detailed description of some neurons, focusing on structural details that were unknown or unresolved by previously published studies. They include the following: (1) dauer-specific branches of the IL2 sensory neurons project into the periphery of anterior sensilla and motor or putative sensory neurons at the sub-lateral cords; (2) ciliated endings of URX sensory neurons are supported by both ILso and AMso socket cells near the amphid openings; (3) variability in amphid sensory dendrites among dauers; and (4) somatic RIP interneurons maintain their projection into the pharyngeal nervous system. Our results support the notion that dauer larvae structurally expand their sensory system to facilitate searching for more favorable environments.https://www.frontiersin.org/articles/10.3389/fnana.2021.732520/fullFIB-SEM3D reconstructionneuroanatomyIL2 branchingamphidsCaenorhabditis elegans (C. elegans) |
| spellingShingle | Sebastian Britz Sebastian Matthias Markert Daniel Witvliet Daniel Witvliet Anna Maria Steyer Sarah Tröger Ben Mulcahy Ben Mulcahy Philip Kollmannsberger Yannick Schwab Mei Zhen Mei Zhen Christian Stigloher Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy Frontiers in Neuroanatomy FIB-SEM 3D reconstruction neuroanatomy IL2 branching amphids Caenorhabditis elegans (C. elegans) |
| title | Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy |
| title_full | Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy |
| title_fullStr | Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy |
| title_full_unstemmed | Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy |
| title_short | Structural Analysis of the Caenorhabditis elegans Dauer Larval Anterior Sensilla by Focused Ion Beam-Scanning Electron Microscopy |
| title_sort | structural analysis of the caenorhabditis elegans dauer larval anterior sensilla by focused ion beam scanning electron microscopy |
| topic | FIB-SEM 3D reconstruction neuroanatomy IL2 branching amphids Caenorhabditis elegans (C. elegans) |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2021.732520/full |
| work_keys_str_mv | AT sebastianbritz structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT sebastianmatthiasmarkert structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT danielwitvliet structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT danielwitvliet structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT annamariasteyer structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT sarahtroger structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT benmulcahy structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT benmulcahy structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT philipkollmannsberger structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT yannickschwab structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT meizhen structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT meizhen structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy AT christianstigloher structuralanalysisofthecaenorhabditiselegansdauerlarvalanteriorsensillabyfocusedionbeamscanningelectronmicroscopy |