<i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples
<i>Broussonetia papyrifera</i> (<i>B.papyrifera</i>), belonging to the Moraceae family, is known to elicit anti-inflammatory, antioxidant, anti-tyrosinase, anticancer, antinociceptive, and antimicrobial effects. The present study has been designed to examine the effects of &l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/7/2/40 |
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| author | Young Han Lee Gaewon Nam Myong-Ki Kim Seok-Cheol Cho Bu Young Choi |
| author_facet | Young Han Lee Gaewon Nam Myong-Ki Kim Seok-Cheol Cho Bu Young Choi |
| author_sort | Young Han Lee |
| collection | DOAJ |
| description | <i>Broussonetia papyrifera</i> (<i>B.papyrifera</i>), belonging to the Moraceae family, is known to elicit anti-inflammatory, antioxidant, anti-tyrosinase, anticancer, antinociceptive, and antimicrobial effects. The present study has been designed to examine the effects of <i>B. papyrifera</i> extract on hair growth through in vitro and clinical samples. Real-time cell growth assay, T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), activation of signal transducer and activator of transcription-6(STAT6) and STAT3 reporter gene function, and Western blotting was performed to examine whether <i>B. papyrifera</i> regulates the expression of target proteins implicated in the proliferation of human hair follicle dermal papilla (hHFDP) cells. In this human trial, using a phototrichogram, the effect of <i>B. papyrifera</i> on hair growth was examined by reconstitution analysis after shaving the hair of the clinical subject’s dorsal skin. <i>B. papyrifera</i> promoted growth equally in hHFDP cells, which is comparable to that of minoxidil and tofacitinib. Treatment with <i>B. papyrifera</i> extract enhanced the TCF/LEF-luciferase activity and increased the level of β-catenin protein. Moreover, <i>B. papyrifera</i> extract significantly suppressed interleukin-4 (IL4)-induced STAT6 phosphorylation. In clinical trial, using a phototrichogram, we assessed the hair density and total hair counts at 0, 6, and 12 weeks after the use of hair tonic containing <i>B. papyrifera</i> extract. After using the hair tonic for 12 weeks, the total hair count was significantly increased as compared with the subjects at the start date (n = 11). <i>B. papyrifera</i> promotes dermal papilla cells proliferation in vitro and clinically among human volunteers through the regulation of WNT-β-catenin and STAT6 pathways. |
| first_indexed | 2024-03-10T19:27:58Z |
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| id | doaj.art-a711b9758a8d48eb9bb39668e5dc79ac |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-10T19:27:58Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-a711b9758a8d48eb9bb39668e5dc79ac2023-11-20T02:26:51ZengMDPI AGCosmetics2079-92842020-06-01724010.3390/cosmetics7020040<i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical SamplesYoung Han Lee0Gaewon Nam1Myong-Ki Kim2Seok-Cheol Cho3Bu Young Choi4Department of Pharmaceutical Science & Engineering, Seowon University, Cheongju 28674, KoreaCosmeceutical R&D Center, Department of Cosmetics, Seowon University, Cheongju 28674, KoreaDepartment of Food Science & Engineering, Seowon University, Cheongju, Chungbuk 28674, KoreaDepartment of Food Science & Engineering, Seowon University, Cheongju, Chungbuk 28674, KoreaDepartment of Pharmaceutical Science & Engineering, Seowon University, Cheongju 28674, Korea<i>Broussonetia papyrifera</i> (<i>B.papyrifera</i>), belonging to the Moraceae family, is known to elicit anti-inflammatory, antioxidant, anti-tyrosinase, anticancer, antinociceptive, and antimicrobial effects. The present study has been designed to examine the effects of <i>B. papyrifera</i> extract on hair growth through in vitro and clinical samples. Real-time cell growth assay, T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), activation of signal transducer and activator of transcription-6(STAT6) and STAT3 reporter gene function, and Western blotting was performed to examine whether <i>B. papyrifera</i> regulates the expression of target proteins implicated in the proliferation of human hair follicle dermal papilla (hHFDP) cells. In this human trial, using a phototrichogram, the effect of <i>B. papyrifera</i> on hair growth was examined by reconstitution analysis after shaving the hair of the clinical subject’s dorsal skin. <i>B. papyrifera</i> promoted growth equally in hHFDP cells, which is comparable to that of minoxidil and tofacitinib. Treatment with <i>B. papyrifera</i> extract enhanced the TCF/LEF-luciferase activity and increased the level of β-catenin protein. Moreover, <i>B. papyrifera</i> extract significantly suppressed interleukin-4 (IL4)-induced STAT6 phosphorylation. In clinical trial, using a phototrichogram, we assessed the hair density and total hair counts at 0, 6, and 12 weeks after the use of hair tonic containing <i>B. papyrifera</i> extract. After using the hair tonic for 12 weeks, the total hair count was significantly increased as compared with the subjects at the start date (n = 11). <i>B. papyrifera</i> promotes dermal papilla cells proliferation in vitro and clinically among human volunteers through the regulation of WNT-β-catenin and STAT6 pathways.https://www.mdpi.com/2079-9284/7/2/40<i>Broussonetia papyrifera</i>hair growthWNT-β-cateninJAK3-STAT6clinicalphototrichogram |
| spellingShingle | Young Han Lee Gaewon Nam Myong-Ki Kim Seok-Cheol Cho Bu Young Choi <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples Cosmetics <i>Broussonetia papyrifera</i> hair growth WNT-β-catenin JAK3-STAT6 clinical phototrichogram |
| title | <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples |
| title_full | <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples |
| title_fullStr | <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples |
| title_full_unstemmed | <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples |
| title_short | <i>Broussonetia papyrifera</i> Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples |
| title_sort | i broussonetia papyrifera i promotes hair growth through the regulation of β catenin and stat6 target proteins a phototrichogram analysis of clinical samples |
| topic | <i>Broussonetia papyrifera</i> hair growth WNT-β-catenin JAK3-STAT6 clinical phototrichogram |
| url | https://www.mdpi.com/2079-9284/7/2/40 |
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