Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone
This study synthesized the NaGdF<sub>4</sub>@NaGdF<sub>4</sub>: Yb, Tm@NaGdF<sub>4</sub>: Yb, Nd upconversion nanoparticles (UCNPs), combined with another three-layer structure NaYF<sub>4</sub>@NaYF<sub>4</sub>: Yb, Er@NaYF<sub>4</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/18/3445 |
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| author | Yang Song Jingyi Jin Liuling Hu Bingqian Hu Mengyao Wang Lilong Guo Xiyan Lv |
| author_facet | Yang Song Jingyi Jin Liuling Hu Bingqian Hu Mengyao Wang Lilong Guo Xiyan Lv |
| author_sort | Yang Song |
| collection | DOAJ |
| description | This study synthesized the NaGdF<sub>4</sub>@NaGdF<sub>4</sub>: Yb, Tm@NaGdF<sub>4</sub>: Yb, Nd upconversion nanoparticles (UCNPs), combined with another three-layer structure NaYF<sub>4</sub>@NaYF<sub>4</sub>: Yb, Er@NaYF<sub>4</sub> UCNPs, with a core-shell-shell structure, effectively suppressing fluorescence quenching and significantly improving upconversion luminescence efficiency. Two types of modified UCNPs were coupled with antibodies against fenpropathrin and procymidone to form signal probes, and magnetic nanoparticles were coupled with antigens of fenpropathrin and procymidone to form capture probes. A rapid and sensitive fluorescence immunoassay for the simultaneous detection of fenpropathrin and procymidone was established based on the principle of specific binding of antigen and antibody and magnetic separation technology. Under the optimal competitive reaction conditions, different concentrations of fenpropathrin and procymidone standards were added to collect the capture probe-signal probe complex. The fluorescence values at 542 nm and 802 nm were measured using 980 nm excitation luminescence. The results showed that the detection limits of fenpropathrin and procymidone were 0.114 µg/kg and 0.082 µg/kg, respectively, with sensitivities of 8.15 µg/kg and 7.98 µg/kg, and they were applied to the detection of fenpropathrin and procymidone in tomatoes, cucumbers, and cabbage. The average recovery rates were 86.5~100.2% and 85.61~102.43%, respectively, with coefficients of variation less than 10%. The results showed good consistency with the detection results of high-performance liquid chromatography, proving that this method has good accuracy and is suitable for the rapid detection of fenpropathrin and procymidone in food. |
| first_indexed | 2024-03-10T22:45:35Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T22:45:35Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-a711bf4fdb0f404d82fca72c566111c92023-11-19T10:43:13ZengMDPI AGFoods2304-81582023-09-011218344510.3390/foods12183445Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and ProcymidoneYang Song0Jingyi Jin1Liuling Hu2Bingqian Hu3Mengyao Wang4Lilong Guo5Xiyan Lv6Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaTianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, ChinaThis study synthesized the NaGdF<sub>4</sub>@NaGdF<sub>4</sub>: Yb, Tm@NaGdF<sub>4</sub>: Yb, Nd upconversion nanoparticles (UCNPs), combined with another three-layer structure NaYF<sub>4</sub>@NaYF<sub>4</sub>: Yb, Er@NaYF<sub>4</sub> UCNPs, with a core-shell-shell structure, effectively suppressing fluorescence quenching and significantly improving upconversion luminescence efficiency. Two types of modified UCNPs were coupled with antibodies against fenpropathrin and procymidone to form signal probes, and magnetic nanoparticles were coupled with antigens of fenpropathrin and procymidone to form capture probes. A rapid and sensitive fluorescence immunoassay for the simultaneous detection of fenpropathrin and procymidone was established based on the principle of specific binding of antigen and antibody and magnetic separation technology. Under the optimal competitive reaction conditions, different concentrations of fenpropathrin and procymidone standards were added to collect the capture probe-signal probe complex. The fluorescence values at 542 nm and 802 nm were measured using 980 nm excitation luminescence. The results showed that the detection limits of fenpropathrin and procymidone were 0.114 µg/kg and 0.082 µg/kg, respectively, with sensitivities of 8.15 µg/kg and 7.98 µg/kg, and they were applied to the detection of fenpropathrin and procymidone in tomatoes, cucumbers, and cabbage. The average recovery rates were 86.5~100.2% and 85.61~102.43%, respectively, with coefficients of variation less than 10%. The results showed good consistency with the detection results of high-performance liquid chromatography, proving that this method has good accuracy and is suitable for the rapid detection of fenpropathrin and procymidone in food.https://www.mdpi.com/2304-8158/12/18/3445core-shell-shellmagnetic separationfenpropathrinprocymidone |
| spellingShingle | Yang Song Jingyi Jin Liuling Hu Bingqian Hu Mengyao Wang Lilong Guo Xiyan Lv Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone Foods core-shell-shell magnetic separation fenpropathrin procymidone |
| title | Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone |
| title_full | Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone |
| title_fullStr | Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone |
| title_full_unstemmed | Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone |
| title_short | Core-Shell-Shell Upconversion Nanomaterials Applying for Simultaneous Immunofluorescent Detection of Fenpropathrin and Procymidone |
| title_sort | core shell shell upconversion nanomaterials applying for simultaneous immunofluorescent detection of fenpropathrin and procymidone |
| topic | core-shell-shell magnetic separation fenpropathrin procymidone |
| url | https://www.mdpi.com/2304-8158/12/18/3445 |
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