Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection
Aquaporins represent proteins contributed to water transport through cell membrane. They are involved in formation and resolution of edema, cell migration and inflammatory reaction. There are only few studies linking the genetic polymorphism of aquaporin 5 (rs3759129 AQP5) and sepsis. At the same tim...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2020-07-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1917 |
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| author | V. M. Pisarev A. G. Chumachenko I. N. Turin R. A. Cherpakov E. V. Elisina E. K. Grigoriev I. A. Aleksandrov A. V. Tutelyan |
| author_facet | V. M. Pisarev A. G. Chumachenko I. N. Turin R. A. Cherpakov E. V. Elisina E. K. Grigoriev I. A. Aleksandrov A. V. Tutelyan |
| author_sort | V. M. Pisarev |
| collection | DOAJ |
| description | Aquaporins represent proteins contributed to water transport through cell membrane. They are involved in formation and resolution of edema, cell migration and inflammatory reaction. There are only few studies linking the genetic polymorphism of aquaporin 5 (rs3759129 AQP5) and sepsis. At the same time, the apparent heterogeneity of patients along the foci of infection may limit finding the most significant association of AQP5 genotypes with the course of infectious complications of critical conditions and restrict further development of rs3759129 AQP5 as a potentially strong marker of sepsis outcome.The purpose of the study was to determine whether the preferential localization of the infection affects the prognostic value of the genetic marker AQP5 (1364A/C, rs3759129) in outcome prediction in sepsis (SEPSIS-3, 2016) patients.Materials and methods. Study groups (n=339) included ICU patients with abdominal sepsis (AS, including pancreatitits, peritonitis, cholecystitis, appendicitis; n=94) sepsis patients with other sources of infections (n=65) and ICU patients without sepsis (n=180). AQP5 polymorphism was studied by analyzing PCR products in a 2% agarose gel using a AQP5 1364A/C specific tetra primer set.Result. Distribution of alleles (A and C) and genotypes (AA, AC and CC) AQP5 1364A/C in patients with sepsis or sepsis subgroups (sepsis with no septic shock and sepsis shock patients) versus control group (healthy volunteers) did not differ. Although there was a trend to preferential survival of sepsis patients with genotype C AQP5 despite the source of infection, only patients with AQP5 CC or AC genotype and abdominal sepsis (Sepsis-3), or a subgroup of the same AQP5 genotype experiencing septic shock, demonstrated increased 30day survival versus AA homozygotic patients (P=0.002).Conclusion. The informative value of detecting the AQP5 CC or AC genotype for prognosis of 30-day survival versus AA homozygotic patients is most significant only in abdominal sepsis patients. |
| first_indexed | 2024-04-10T01:27:25Z |
| format | Article |
| id | doaj.art-a711e6e1ba7a4b38ba608a2dfd628a44 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2024-04-10T01:27:25Z |
| publishDate | 2020-07-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-a711e6e1ba7a4b38ba608a2dfd628a442023-03-13T09:32:56ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102020-07-01163163310.15360/1813-9779-2020-3-16-331740Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of InfectionV. M. Pisarev0A. G. Chumachenko1I. N. Turin2R. A. Cherpakov3E. V. Elisina4E. K. Grigoriev5I. A. Aleksandrov6A. V. Tutelyan7НИИ общей реаниматологии им. В. А. Неговского, ФНКЦ РРНИИ общей реаниматологии им. В. А. Неговского, ФНКЦ РРГородская клиническая больница № 40 Департамента здравоохранения г. МосквыГородская клиническая больница № 40 Департамента здравоохранения г. МосквыНИИ общей реаниматологии им. В. А. Неговского, ФНКЦ РРНИИ общей реаниматологии им. В. А. Неговского, ФНКЦ РРНациональный медицинский исследовательский центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава РоссииНациональный медицинский исследовательский центр детской гематологии, онкологии и иммунологии им. Д. Рогачева Минздрава России; Центральный НИИ эпидемиологии РоспотребнадзораAquaporins represent proteins contributed to water transport through cell membrane. They are involved in formation and resolution of edema, cell migration and inflammatory reaction. There are only few studies linking the genetic polymorphism of aquaporin 5 (rs3759129 AQP5) and sepsis. At the same time, the apparent heterogeneity of patients along the foci of infection may limit finding the most significant association of AQP5 genotypes with the course of infectious complications of critical conditions and restrict further development of rs3759129 AQP5 as a potentially strong marker of sepsis outcome.The purpose of the study was to determine whether the preferential localization of the infection affects the prognostic value of the genetic marker AQP5 (1364A/C, rs3759129) in outcome prediction in sepsis (SEPSIS-3, 2016) patients.Materials and methods. Study groups (n=339) included ICU patients with abdominal sepsis (AS, including pancreatitits, peritonitis, cholecystitis, appendicitis; n=94) sepsis patients with other sources of infections (n=65) and ICU patients without sepsis (n=180). AQP5 polymorphism was studied by analyzing PCR products in a 2% agarose gel using a AQP5 1364A/C specific tetra primer set.Result. Distribution of alleles (A and C) and genotypes (AA, AC and CC) AQP5 1364A/C in patients with sepsis or sepsis subgroups (sepsis with no septic shock and sepsis shock patients) versus control group (healthy volunteers) did not differ. Although there was a trend to preferential survival of sepsis patients with genotype C AQP5 despite the source of infection, only patients with AQP5 CC or AC genotype and abdominal sepsis (Sepsis-3), or a subgroup of the same AQP5 genotype experiencing septic shock, demonstrated increased 30day survival versus AA homozygotic patients (P=0.002).Conclusion. The informative value of detecting the AQP5 CC or AC genotype for prognosis of 30-day survival versus AA homozygotic patients is most significant only in abdominal sepsis patients.https://www.reanimatology.com/rmt/article/view/1917полиморфизм геновaqp5rs3759129сепсисабдоминальный сепсискритические состояния |
| spellingShingle | V. M. Pisarev A. G. Chumachenko I. N. Turin R. A. Cherpakov E. V. Elisina E. K. Grigoriev I. A. Aleksandrov A. V. Tutelyan Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection Общая реаниматология полиморфизм генов aqp5 rs3759129 сепсис абдоминальный сепсис критические состояния |
| title | Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection |
| title_full | Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection |
| title_fullStr | Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection |
| title_full_unstemmed | Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection |
| title_short | Prognostic Value of a Genetic Polymorphism in Promotor Region of AQP5 in Sepsis Depends on the Source of Infection |
| title_sort | prognostic value of a genetic polymorphism in promotor region of aqp5 in sepsis depends on the source of infection |
| topic | полиморфизм генов aqp5 rs3759129 сепсис абдоминальный сепсис критические состояния |
| url | https://www.reanimatology.com/rmt/article/view/1917 |
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