Genetic background influences arterial vasomotor function in male and female mice
Abstract The purpose of this study was to assess the influence of genetic background and sex on nitric oxide (NO)‐mediated vasomotor function in arteries from different vascular territories. Vasomotor function was assessed in thoracic aorta, abdominal aorta, carotid arteries, and femoral arteries fr...
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15824 |
| _version_ | 1797397431056334848 |
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| author | Dylan Holly Hyoseon Kim Christopher R. Woodman Michael P. Massett |
| author_facet | Dylan Holly Hyoseon Kim Christopher R. Woodman Michael P. Massett |
| author_sort | Dylan Holly |
| collection | DOAJ |
| description | Abstract The purpose of this study was to assess the influence of genetic background and sex on nitric oxide (NO)‐mediated vasomotor function in arteries from different vascular territories. Vasomotor function was assessed in thoracic aorta, abdominal aorta, carotid arteries, and femoral arteries from the following mouse strains: SJL/J, DBA/2J, NZW/LacJ, and C57BL/6J. Contractile responses were assessed using the α1‐adrenergic receptor agonist phenylephrine (PE, 10−9–10−5 M). Vasorelaxation responses were assessed by examining relaxation to an endothelium‐dependent vasodilator acetylcholine (ACh, 10−9–10−5 M) and an endothelium‐independent vasodilator sodium nitroprusside (SNP, 10−9–10−5 M). To evaluate the role of NO, relaxation responses to ACh and SNP were assessed in the absence or presence of a nitric oxide synthase inhibitor (N omega‐nitro‐l‐arginine methyl ester hydrochloride: 10−4 M). Vasomotor responses to ACh and PE varied across strains and among the arteries tested with some strains exhibiting artery‐specific impairment. Results indicated some concentration–response heterogeneity in response to ACh and SNP between vessels from females and males, but no significant differences in responses to PE. Collectively, these findings indicate that vasomotor responses vary by genetic background, sex, and artery type. |
| first_indexed | 2024-03-09T01:10:51Z |
| format | Article |
| id | doaj.art-a712e08780a34ec085149fb8d3136150 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:10:51Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-a712e08780a34ec085149fb8d31361502023-12-11T07:16:19ZengWileyPhysiological Reports2051-817X2023-10-011119n/an/a10.14814/phy2.15824Genetic background influences arterial vasomotor function in male and female miceDylan Holly0Hyoseon Kim1Christopher R. Woodman2Michael P. Massett3Department of Kinesiology and Sport Management Texas A&M University College Station Texas USADepartment of Kinesiology and Sport Management Texas Tech University Lubbock Texas USADepartment of Kinesiology and Sport Management Texas A&M University College Station Texas USADepartment of Kinesiology and Sport Management Texas Tech University Lubbock Texas USAAbstract The purpose of this study was to assess the influence of genetic background and sex on nitric oxide (NO)‐mediated vasomotor function in arteries from different vascular territories. Vasomotor function was assessed in thoracic aorta, abdominal aorta, carotid arteries, and femoral arteries from the following mouse strains: SJL/J, DBA/2J, NZW/LacJ, and C57BL/6J. Contractile responses were assessed using the α1‐adrenergic receptor agonist phenylephrine (PE, 10−9–10−5 M). Vasorelaxation responses were assessed by examining relaxation to an endothelium‐dependent vasodilator acetylcholine (ACh, 10−9–10−5 M) and an endothelium‐independent vasodilator sodium nitroprusside (SNP, 10−9–10−5 M). To evaluate the role of NO, relaxation responses to ACh and SNP were assessed in the absence or presence of a nitric oxide synthase inhibitor (N omega‐nitro‐l‐arginine methyl ester hydrochloride: 10−4 M). Vasomotor responses to ACh and PE varied across strains and among the arteries tested with some strains exhibiting artery‐specific impairment. Results indicated some concentration–response heterogeneity in response to ACh and SNP between vessels from females and males, but no significant differences in responses to PE. Collectively, these findings indicate that vasomotor responses vary by genetic background, sex, and artery type.https://doi.org/10.14814/phy2.15824aortacarotid arteryendotheliumfemoral arteryinbred micesex differences |
| spellingShingle | Dylan Holly Hyoseon Kim Christopher R. Woodman Michael P. Massett Genetic background influences arterial vasomotor function in male and female mice Physiological Reports aorta carotid artery endothelium femoral artery inbred mice sex differences |
| title | Genetic background influences arterial vasomotor function in male and female mice |
| title_full | Genetic background influences arterial vasomotor function in male and female mice |
| title_fullStr | Genetic background influences arterial vasomotor function in male and female mice |
| title_full_unstemmed | Genetic background influences arterial vasomotor function in male and female mice |
| title_short | Genetic background influences arterial vasomotor function in male and female mice |
| title_sort | genetic background influences arterial vasomotor function in male and female mice |
| topic | aorta carotid artery endothelium femoral artery inbred mice sex differences |
| url | https://doi.org/10.14814/phy2.15824 |
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