A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an important role in the human body and monitoring its level is meaningful due to the relationship between its level and diseases. A fluorescent sensor (<b>CMB</b>) based on coumarin was designed and its ability...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3352 |
| _version_ | 1797531671783800832 |
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| author | Yingying Zuo Yang Jiao Chunming Ma Chunying Duan |
| author_facet | Yingying Zuo Yang Jiao Chunming Ma Chunying Duan |
| author_sort | Yingying Zuo |
| collection | DOAJ |
| description | Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an important role in the human body and monitoring its level is meaningful due to the relationship between its level and diseases. A fluorescent sensor (<b>CMB</b>) based on coumarin was designed and its ability for detecting hydrogen peroxide by fluorescence signals was also studied. The <b>CMB</b> showed an approximate 25-fold fluorescence enhancement after adding H<sub>2</sub>O<sub>2</sub> due to the interaction between the <b>CMB</b> and H<sub>2</sub>O<sub>2</sub> and had the potential for detecting physiological H<sub>2</sub>O<sub>2</sub>. It also showed good biocompatibility and permeability, allowing it to penetrate cell membranes and zebrafish tissues, thus it can perform fluorescence imaging of H<sub>2</sub>O<sub>2</sub> in living cells and zebrafish. This probe is a promising tool for monitoring the level of H<sub>2</sub>O<sub>2</sub> in related physiological and pathological research. |
| first_indexed | 2024-03-10T10:47:04Z |
| format | Article |
| id | doaj.art-a7187fd241e14e1fbf4622e552cfb583 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:47:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a7187fd241e14e1fbf4622e552cfb5832023-11-21T22:32:10ZengMDPI AGMolecules1420-30492021-06-012611335210.3390/molecules26113352A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-ImagingYingying Zuo0Yang Jiao1Chunming Ma2Chunying Duan3State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, ChinaState Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, ChinaState Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, ChinaState Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, ChinaHydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an important role in the human body and monitoring its level is meaningful due to the relationship between its level and diseases. A fluorescent sensor (<b>CMB</b>) based on coumarin was designed and its ability for detecting hydrogen peroxide by fluorescence signals was also studied. The <b>CMB</b> showed an approximate 25-fold fluorescence enhancement after adding H<sub>2</sub>O<sub>2</sub> due to the interaction between the <b>CMB</b> and H<sub>2</sub>O<sub>2</sub> and had the potential for detecting physiological H<sub>2</sub>O<sub>2</sub>. It also showed good biocompatibility and permeability, allowing it to penetrate cell membranes and zebrafish tissues, thus it can perform fluorescence imaging of H<sub>2</sub>O<sub>2</sub> in living cells and zebrafish. This probe is a promising tool for monitoring the level of H<sub>2</sub>O<sub>2</sub> in related physiological and pathological research.https://www.mdpi.com/1420-3049/26/11/3352hydrogen peroxidereactive oxygen speciesfluorescenceimaging |
| spellingShingle | Yingying Zuo Yang Jiao Chunming Ma Chunying Duan A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging Molecules hydrogen peroxide reactive oxygen species fluorescence imaging |
| title | A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging |
| title_full | A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging |
| title_fullStr | A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging |
| title_full_unstemmed | A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging |
| title_short | A Novel Fluorescent Probe for Hydrogen Peroxide and Its Application in Bio-Imaging |
| title_sort | novel fluorescent probe for hydrogen peroxide and its application in bio imaging |
| topic | hydrogen peroxide reactive oxygen species fluorescence imaging |
| url | https://www.mdpi.com/1420-3049/26/11/3352 |
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